|本期目录/Table of Contents|

[1]尚洁,赵海燕,朱武政.枸杞DFR基因cDNA片段的克隆及生物信息学分析[J].江苏农业科学,2013,41(10):33-37.
　Shang Jie,et al.Cloning and bioinformatics analysis of DFR gene in Lycium barbarum L.[J].Jiangsu Agricultural Sciences,2013,41(10):33-37.
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枸杞DFR基因cDNA片段的克隆及生物信息学分析(PDF)
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第41卷
期数:
2013年10期
页码:
33-37
栏目:
生物技术
出版日期:
2013-10-25

文章信息/Info

Title:
Cloning and bioinformatics analysis of DFR gene in Lycium barbarum L.
作者:
尚洁1; 赵海燕1; 朱武政2
1.北方民族大学生物科学与工程学院，宁夏银川 750021； 2.西南民族大学生命科学与技术学院，四川成都 610041
Author(s):
Shang Jie; et al
关键词:
枸杞; DFR基因; 克隆; 生物信息学分析
Keywords:
-
分类号:
S567.1+90.1；Q785
DOI:
-
文献标志码:
A
摘要:
二氢黄酮醇4-还原酶(DFR，1.1.1.219)是类黄酮代谢中花青素合成的一种主要调控酶，它依赖NADPH还原二氢黄酮醇为无色花色素。以枸杞为材料，利用序列同源原理和RT-PCR技术得到1条DFR基因，并对其进行生物信息学分析。结果表明，该基因的cDNA 片段长为893 bp，编码297个氨基酸，蛋白分子量为33.94 ku，等电点为6.08。氨基酸序列和结构分析显示DFR基因具有典型的NAD(P)-binding Rossmann-fold结构域。LyDFR蛋白很可能定位在细胞质。二级结构和三级结构进行预测，发现LyDFR蛋白中无规则卷曲114个，α螺旋和β折叠分别为124个和18个，延伸链41个。系统进化分析表明，枸杞LyDFR基因与马铃薯和番茄的DFR基因具有很高的同源性。这个基因已在GenBank上注册，基因序列登录号为KC795560。
Abstract:
-

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备注/Memo

备注/Memo:
收稿日期：2013-03-22
基金项目：北方民族大学校级科研项目（编号：2010Y046）。
作者简介：尚洁（1979—），女，宁夏中卫人，硕士，讲师，研究方向为植物分子生物学。E-mail：shangjie@126.com。
更新日期/Last Update: 2013-10-25