|本期目录/Table of Contents|

[1]龚志云,张明亮,薛超,等.小桐子高分辨率细胞学图的建立[J].江苏农业科学,2013,41(11):377-381.
 Gong Zhiyun,et al.Establishment of high-resolution cytological map in Jatropha curcas L.[J].Jiangsu Agricultural Sciences,2013,41(11):377-381.
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小桐子高分辨率细胞学图的建立(PDF)
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第41卷
期数:
2013年11期
页码:
377-381
栏目:
资源与环境
出版日期:
2013-11-25

文章信息/Info

Title:
Establishment of high-resolution cytological map in Jatropha curcas L.
作者:
龚志云 张明亮 薛超 石国新 刘秀秀
扬州大学江苏省作物遗传生理重点实验室/教育部植物功能基因组学重点实验室,江苏扬州 225009
Author(s):
Gong Zhiyunet al
关键词:
小桐子高分辨率染色体
Keywords:
-
分类号:
A
DOI:
-
文献标志码:
Q943
摘要:
摸索出小桐子高分辨率细胞学图的建立方法,在小桐子的染色体制片过程中,根尖的长短、预处理的时间、酶解相关因素对试验影响较大。小桐子根尖长度为1.5~2.0 cm,0.002 mol/L 8- 羟基喹啉预处理2 h时,37 ℃酶解1.5 h后得的染色体浓缩程度适宜,分散均匀、形态清晰、细胞分裂相较多,没有细胞质覆盖。小桐子花粉母细胞直径在2 mm左右能获得较多粗线期染色体浓缩的分裂相。
Abstract:
-

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:2013-05-02
基金项目:国家“973”计划(编号:2010CB126603);教育部霍英东教育基金会高等院校青年教师基金(编号:131030)。
作者简介:龚志云(1975—),女,博士,主要从事植物染色体分子生物学研究。Tel:(0514)87979304;E-mail:zygong@yzu.edu.cn。
更新日期/Last Update: 2013-11-25