|本期目录/Table of Contents|

[1]马晓琴,黄林,刘文静,等.牦牛和黄牛Prdm9基因锌指域序列的比较[J].江苏农业科学,2014,42(04):28-30.
　Ma Xiaoqin,et al.Analysis of zinc finger domain sequence of Prdm9 gene in yak and cattle[J].Jiangsu Agricultural Sciences,2014,42(04):28-30.
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牦牛和黄牛Prdm9基因锌指域序列的比较(PDF)
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第42卷
期数:
2014年04期
页码:
28-30
栏目:
生物技术
出版日期:
2014-04-25

文章信息/Info

Title:
Analysis of zinc finger domain sequence of Prdm9 gene in yak and cattle
作者:
马晓琴1;  黄林1;  刘文静2;  林亚秋1;  金素钰1;  郑玉才1
1.西南民族大学生命科学与技术学院，四川成都 610041； 2.西南科技大学生命科学与工程学院，四川绵阳 621010
Author(s):
Ma Xiaoqin; et al
关键词:
牦牛; 黄牛; 杂交雄性不育; 睾丸; Prdm9基因
Keywords:
-
分类号:
S823.8+52；S823.8+12
DOI:
-
文献标志码:
A
摘要:
为了研究牦牛杂交雄性不育的分子机制，采用3′-RACE方法分别从牦牛、黄牛睾丸组织的总RNA中获取其Prdm9基因的部分cDNA序列，长度分别为1 548、1 392 bp，包含全部的锌指域。对比分析显示：牦牛、黄牛的Prdm9基因均含有5个连续的C2H2型锌指，每个锌指均由21个氨基酸构成，锌指间是高度保守序列TGEKPYV，并且锌指域从这段高度保守序列开始；牦牛、黄牛各自Prdm9基因中的5个锌指间在氨基酸构成上存在差异，并且二者在整个锌指域上有6个氨基酸差异，其中5个都在锌指中的重要正向选择位点处，推测这些氨基酸差异可能与牦牛种间杂交后代的雄性不育有关。
Abstract:
-

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期：2013-12-11
基金项目：国家自然科学基金(编号：31240053)；中央高校基本科研业务费资助课题(编号：12NZYTH06)，西南民族大学研究生创新型科研项目(编号：CX2013SZ55)。
作者简介：马晓琴(1987—)，女，甘肃武威人，硕士，主要从事动物生物化学与分子遗传学研究。E-mail：maxiaoqin1987@hotmail.com。
通信作者：郑玉才(1965—)，男，内蒙古海拉尔人，博士，教授，主要从事牦牛的研究。E-mail：yucaizheng65@hotmail.com。
更新日期/Last Update: 2014-04-25