|本期目录/Table of Contents|

[1]徐俊华.玉米ZmCIPK基因的克隆及表达分析[J].江苏农业科学,2014,42(05):51-53.
　Xu Junhua.Cloning and expression analysis of ZmCIPK gene in maize[J].Jiangsu Agricultural Sciences,2014,42(05):51-53.
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玉米ZmCIPK基因的克隆及表达分析(PDF)
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第42卷
期数:
2014年05期
页码:
51-53
栏目:
生物技术
出版日期:
2014-05-25

文章信息/Info

Title:
Cloning and expression analysis of ZmCIPK gene in maize
作者:
徐俊华
淄博职业学院，山东淄博 255314
Author(s):
Xu Junhua
关键词:
玉米; ZmCIPK基因; 表达模式; 逆境
Keywords:
-
分类号:
A
DOI:
-
文献标志码:
Q786
摘要:
根据玉米CIPK基因EST序列设计引物，采用RT-PCR技术从玉米中克隆了1个ZmCIPK基因。分析结果表明，ZmCIPK基因cDNA全长1 748 bp，5′-非编码区长115 bp，3′-非编码区长508 bp，编码区长1 125 bp，编码374个氨基酸，预测分子量为41.72 Ku，等电点为6.96。氨基酸同源性分析发现，ZmCIPK蛋白与高粱的CIPK蛋白同源性较高。实时定量PCR检测表明，ZmCIPK基因的表达受盐胁迫和低温诱导，说明玉米ZmCIPK基因可能参与玉米对逆境胁迫的应答。
Abstract:
-

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期：2014-01-07
基金项目：淄博职业学院科技基金（编号：KJ13306）。
作者简介：徐俊华（1968—），女，山东人，硕士，讲师，主要从事生物学、动植物学、微生物学等学科的教学与研究。E-mail：1697986428@qq.com。
更新日期/Last Update: 2014-05-25