|本期目录/Table of Contents|

[1]唐立群,黄世文,侯雨萱.水稻种子酵母双杂交体系的构建及种子特异表达蛋白ONAC023互作蛋白的鉴定[J].江苏农业科学,2014,42(09):16-20.
　Tang Liqun,et al.Establishment and utilization of a yeast two-hybrid system for rice seed：Taking ONAC023 as an example[J].Jiangsu Agricultural Sciences,2014,42(09):16-20.
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水稻种子酵母双杂交体系的构建及种子特异表达蛋白ONAC023互作蛋白的鉴定(PDF)
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第42卷
期数:
2014年09期
页码:
16-20
栏目:
生物技术
出版日期:
2014-09-25

文章信息/Info

Title:
Establishment and utilization of a yeast two-hybrid system for rice seed：Taking ONAC023 as an example
作者:
唐立群;  黄世文;  侯雨萱
水稻生物学国家重点实验室/中国水稻研究所稻作技术研究与发展中心，浙江杭州 310006
Author(s):
Tang Liqun; et al
关键词:
水稻; 酵母双杂交; 种子发育; NAC
Keywords:
-
分类号:
S511.035.1
DOI:
-
文献标志码:
A
摘要:
酵母双杂交是常用的蛋白-蛋白检测技术。以水稻受精5 d的幼嫩种子为材料，构建了适用于水稻种子发育相关蛋白的互作蛋白酵母双杂交筛选平台，cDNA文库容量达到1.1×106，平均插入大小约为750 bp，开发了改良的酵母菌落PCR方法，用于文库插入片段的扩增，大大提高了筛选效率。NAC是植物特有的一类转录因子，在植物发育、抗逆等多个方面发挥着重要功能。RT-PCR结果显示，NAC家族成员ONAC023在种子中特异表达，可能调控种子早期发育。以ONAC023为诱饵，成功地筛选到21个互作蛋白候选基因，其中包含1个DNA linding protein，推测其可能是ONAC023蛋白复合物的成员。
Abstract:
-

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期：2014-05-07
基金项目：国家科技支撑计划(编号：2012BAD19B03)；国家转基因生物新品种培育专项(编号：2011ZX08010-005)；中国农业科学院科技创新工程。
作者简介：唐立群(1987—)，女，湖南东安人，硕士，从事水稻基因功能研究。E-mail：liquntang2013@126.com 。
通信作者：候雨萱，博士，助理研究员，从事水稻基因功能研究。E-mail：houyuxuan@caas.cn。
更新日期/Last Update: 2014-09-25