|本期目录/Table of Contents|

[1]李小玉,张晓峰,杜伟,等.桑树MaSAUR2基因的克隆、序列特征及时间特异性表达[J].江苏农业科学,2014,42(12):34-38.
　Li Xiaoyu,et al.Cloning，sequence characteristics and time-specific expression of MaSAUR2 gene in mulberry[J].Jiangsu Agricultural Sciences,2014,42(12):34-38.
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桑树MaSAUR2基因的克隆、序列特征及时间特异性表达(PDF)
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第42卷
期数:
2014年12期
页码:
34-38
栏目:
生物技术
出版日期:
2014-12-25

文章信息/Info

Title:
Cloning，sequence characteristics and time-specific expression of MaSAUR2 gene in mulberry
作者:
李小玉1;  张晓峰1;  杜伟1;  聂浩1;  唐壮1;  班月圆1;  杜小龙1;  程嘉翎1; 2
1.江苏科技大学，江苏镇江 212018； 2.中国农业科学院蚕业研究所，江苏镇江 212018
Author(s):
Li Xiaoyu; et al
关键词:
桑树; MaSAUR2基因; 基因克隆; 序列特征; 实时定量PCR
Keywords:
-
分类号:
S888.2；Q943.2
DOI:
-
文献标志码:
A
摘要:
SAUR是生长素早期响应基因，能够被生长素快速诱导表达，本研究从桑树绿枝扦插的插穗基部皮层中克隆得到1个桑树SAUR家族基因，命名为MaSAUR2(GenBank登录号：KC852881)。该基因序列全长1 186 bp，ORF全长549 bp，编码182个氨基酸，蛋白质分子质量为20 700，无信号肽与跨膜区域，在第82 aa至138 aa之间有1个保守的SAUR-specific结构域。桑树绿枝插穗基部经吲哚-3-乙酸(IAA)诱导处理，插穗基部皮层MaSAUR2基因的表达水平在5 min内显著上升；在绿枝扦插生根过程中，不定根长出皮层时MaSAUR2基因表达量显著升高。推测 MaSAUR2 参与了桑树扦插过程中不定根的形成。
Abstract:
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备注/Memo

备注/Memo:
收稿日期：2014-01-09
资助项目：现代农业产业技术体系专项(编号：CARS-22)。
作者简介：李小玉(1989—)，女，河南濮阳人，硕士研究生，主要从事桑树扦插生根分子机理研究。E-mail：lixiaoyupy@163.com。
通信作者：程嘉翎，研究员，硕士生导师，主要从事桑树生理与栽培研究。Tel：(0511)85616573；E-mail：chengjialing@163.com。
更新日期/Last Update: 2014-12-25