柴达木盆地梭梭耐盐相关基因<i>PrxQ</i>的克隆<br/>及其蛋白结构预测 -《江苏农业科学》

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Title:: Cloning of salt-resistant PrxO gene of Haloxylon ammodendron in Chaidamu Basin and prediction of its protein structure

作者:: 马玉花; 冶贵生; 冯志鹏; 青海大学农牧学院，青海西宁 810016

Author(s):: Ma Yuhua; et al

关键词:: 梭梭; PrxQ基因; 序列分析; 蛋白结构

Keywords:: -

分类号:: S718.43

DOI:: -

文献标志码:: A

摘要:: 对柴达木盆地梭梭的PrxQ基因进行扩增，并利用生物软件对PrxQ基因序列进行分析，对蛋白结构进行预测。结果表明：柴达木盆地梭梭PrxQ基因长度为657 bp，编码218个氨基酸；PrxQ蛋白亲水性、二级结构、亚细胞定位、信号肽、跨膜螺旋区、三级结构预测结果显示，柴达木盆地梭梭PrxQ蛋白亚细胞定位于叶绿体，蛋白二级结构主要为无规则卷曲；PrxQ具有2个N-糖基化位点、7个蛋白激酶C磷酸化位点、2个酪蛋白激酶Ⅱ磷酸化位点，此外PrxQ蛋白的亲水性较强，无跨膜螺旋区。柴达木盆地梭梭PrxQ蛋白三维结构预测结果显示，PrXQ蛋白三维结构由α螺旋、β折叠、无规则卷曲互相盘绕而成。

Abstract:: -

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