|本期目录/Table of Contents|

[1]王珊珊,徐海洋,王世明.酵母穿梭质粒pXZ200的构建[J].江苏农业科学,2015,43(08):31-34.
 Wang Shanshan,et al.Construction of yeast shuttle plasmid pXZ200[J].Jiangsu Agricultural Sciences,2015,43(08):31-34.
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酵母穿梭质粒pXZ200的构建(PDF)
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第43卷
期数:
2015年08期
页码:
31-34
栏目:
生物技术
出版日期:
2015-08-25

文章信息/Info

Title:
Construction of yeast shuttle plasmid pXZ200
作者:
王珊珊 徐海洋 王世明
南京理工大学环境与生物工程学院,江苏南京 210094
Author(s):
Wang Shanshanet al
关键词:
酿酒酵母同源重组穿梭质粒PCR技术pXZ200
Keywords:
-
分类号:
Q936
DOI:
-
文献标志码:
A
摘要:
利用酿酒酵母高效的同源重组能力,通过PCR扩增技术,将复制子pBBR1片段、KanR(卡纳霉素抗性)片段、酵母复制子2 μ片段这3个两端含有30 bp同源臂的片段电转入酿酒酵母,通过同源重组获得宿主更具广谱性的革兰氏阴性细菌-啤酒酵母穿梭质粒pXZ200。经检测,pXZ200能够在恶臭假单胞杆菌KT2440和大肠杆菌中稳定复制。
Abstract:
-

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:2014-08-21
基金项目:中央高校基本科研业务费专项资金(编号:30920130121013)。
作者简介:王珊珊,女,硕士,从事微生物生理生化研究。E-mail:wangshanshanb305@163.com。
通信作者:王世明,博士,副教授,从事微生物生理生化研究。E-mail:wangshanshanb305@163.com。
更新日期/Last Update: 2015-08-25