|本期目录/Table of Contents|

[1]高俊,王舒雅,冯迟,等.大孔树脂分离富集葛根中葛根素及大豆苷元[J].江苏农业科学,2015,43(09):292-296.
 Gao Jun,et al.Seperation and enrichment of puerarin and soybean glycosides from root of radix puerariae by macroporous resin[J].Jiangsu Agricultural Sciences,2015,43(09):292-296.
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第43卷
期数:
2015年09期
页码:
292-296
栏目:
贮藏与加工
出版日期:
2015-09-25

文章信息/Info

Title:
Seperation and enrichment of puerarin and soybean glycosides from root of radix puerariae by macroporous resin
作者:
高俊12 王舒雅12 冯迟12 雍晓雨12 周俊12 刘晓宁12 张亚兵12 谢欣欣12 陈怡露1 郑涛12
1.南京工业大学生物与制药工程学院,江苏南京 211816; 2.南京工业大学生物能源研究所,江苏南京 211816
Author(s):
Gao Junet al
关键词:
葛根素大豆苷元大孔树脂吸附解吸分离富集工艺条件
Keywords:
-
分类号:
R284.2
DOI:
-
文献标志码:
A
摘要:
研究8种不同极性大孔吸附树脂对葛根素、大豆苷元的吸附、解吸性能,筛选出分离富集葛根素、大豆苷元的最佳树脂HP20。利用HP20大孔吸附树脂吸附富集葛根中2种异黄酮的过程为:上样液用盐酸调节pH值为6,然后采用HP20大孔吸附树脂在5 ℃的条件下进行吸附。结果表明,HP20大孔吸附树脂对葛根素、大豆苷元的饱和吸附量分别为(71.78±0.75)、(21.77±0.01)mg/g;吸附饱和后用去离子水洗涤树脂以除去杂质,在25 ℃条件下用70%乙醇解吸,得葛根素、大豆苷元,解吸率分别为63.39%、80.68%,葛根素纯度由8.62%提高到35.26%,大豆苷元纯度由0.27%提高到10.17%。
Abstract:
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备注/Memo

备注/Memo:
收稿日期:2014-09-16
基金项目:国家“973”计划(编号:2013CB733502);江苏省自然科学基金青年项目(编号:BK20130931、BK20130932)。
作者简介:高俊(1989—),男,江苏泰州人,硕士研究生,主要从事植物活性成分分离及天然药物提取研究。E-mail:gj1981118@njtech.edu.cn。
通信作者:郑涛,博士,教授,主要从事生物燃气、植物药研究与开发、微生物燃料电池研究。E-mail:zhengtao@njtech.edu
更新日期/Last Update: 2015-09-25