翘嘴鳜胚胎发育期间酸性和碱性磷酸酶活性的变化-《江苏农业科学》

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Title:: Changes of acid phosphatase and alkaline phosphatase activities during embryonic development of mandarin fish，Siniperca chuatsi

作者:: 邹立军; 庄远红; 龚婧; 吉璐; 黄美玲; 湖南第一师范学院基础生物学实验室，湖南长沙 410205

Author(s):: Zou Lijun; et al

关键词:: 翘嘴鳜; 胚胎发育; 酸性磷酸酶; 碱性磷酸酶

Keywords:: -

分类号:: S917

DOI:: -

文献标志码:: A

摘要:: 酸性磷酸酶和碱性磷酸酶作为一种磷酸单酯水解酶，在蛋白(酶)的去磷酸化过程中起着非常重要的作用，研究发现磷酸酶是动物发育过程中物质代谢的重要调控酶。采用磷酸苯二钠比色法检测翘嘴鳜(Siniperca chuatsi)胚胎发育期间酸性磷酸酶和碱性磷酸酶的活性变化。结果显示，胚胎受精后进行细胞分裂时的酸性和碱性磷酸酶活性较低，分别为(0.436±0.051) U/g和(0.061±0.009) U/g，发育至囊胚期时酶活性均有微量下降；而从原肠胚时期开始，酸性和碱性磷酸酶的活性随胚胎发育的进行呈现上升趋势，在出膜时期达到最高，分别为(1.076±0.063) U/g和(0.335±0.021) U/g，酶活性显著增强(P<0.05)。这一结果表明，翘嘴鳜胚胎在原肠胚期合成了磷酸酶，且磷酸酶在胚胎发育期间的细胞增殖、信号传导和物质代谢等方面可能发挥重要作用。

Abstract:: -

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