|本期目录/Table of Contents|

[1]孙晓梅,黄金光.禾谷镰刀菌甾醇14α脱甲基酶基因cDNA克隆及生物信息学分析[J].江苏农业科学,2016,44(03):31-36.
 Sun Xiaomei,et al.cDNA cloning and bioinformatics analysis of sterol 14α-demethylase gene in Fusarium graminearum[J].Jiangsu Agricultural Sciences,2016,44(03):31-36.
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禾谷镰刀菌甾醇14α脱甲基酶基因cDNA克隆
及生物信息学分析
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第44卷
期数:
2016年03期
页码:
31-36
栏目:
生物技术
出版日期:
2016-03-25

文章信息/Info

Title:
cDNA cloning and bioinformatics analysis of sterol 14α-demethylase gene in Fusarium graminearum
作者:
孙晓梅1 黄金光2
1.青岛农业大学动漫与传媒学院,山东青岛 266109;
2.山东省植物病虫害综合防控重点实验室/青岛农业大学农学与植物保护学院,山东青岛 266109
Author(s):
Sun Xiaomeiet al
关键词:
禾谷镰刀菌甾醇14α脱甲基酶基因克隆抗药性生物信息学分析cDNA
Keywords:
-
分类号:
S182;Q785
DOI:
-
文献标志码:
A
摘要:
克隆获得禾谷镰刀菌(Fusarium graminearum)2个甾醇14α脱甲基酶CYP51蛋白(CYP51A、CYP51B)基因cDNA序列,明确其氨基酸序列典型特征,为研究蛋白质结构及其抗药性机制奠定基础。采用RT-PCR技术,克隆2个CYP51蛋白基因cDNA序列,利用相关生物信息软件对其序列进行生物信息学分析。结果克隆到2条cDNA序列,长度分别为1 524、1 581 bp,分别编码507、526个氨基酸;2个蛋白分子量分别为57.5、59.3 ku,等电点分别为6
Abstract:
-

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:2015-03-11
基金项目:国家自然科学基金(编号:31471735);国家科技基础条件平台子项目(编号:2005DKA21207-17);山东省“泰山学者”建设工程专项经费(编号:TSSD201316);青岛农业大学高层次人才科研基金(编号:631217)。
作者简介:孙晓梅(1975—),女,山东莱州人,硕士,副教授,主要从事生物信息学研究。E-mail:xmsun@qau.edu.cn。
通信作者:黄金光,博士,教授,主要从事蛋白质结构生物学、分子植物病理学研究。E-mail:jghuang@qau.edu.cn。
更新日期/Last Update: 2016-03-25