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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:

第44卷

期数:

2016年04期

页码:

16-22

栏目:

生物技术

出版日期:

2016-04-25

文章信息/Info

Title:

Cloning of maize transcription factor gene ZmASR1 and construction of plant expression vector

作者:

张丽丽;  李博;  谭燕华;  曹扬;  易小平

中国热带农业科学院热带生物技术研究所，海南海口 571101

Author(s):

Zhang Lili; et al

关键词:

玉米; Ubiquitin启动子; ZmASR1基因; 生物信息学; 植物表达载体

Keywords:

-

分类号:

Q785

DOI:

-

文献标志码:

A

摘要:

为深入研究玉米转录因子基因ZmASR1的功能，提高玉米株系的产量和抗逆性，根据http：//www.maizegdb.org/ 网站上公布的玉米自交系B73的ZmASR1序列和Cornejo发表的Ubiquitin启动子的序列，设计引物从玉米自交系B73的DNA序列中克隆了ZmASR1基因和Ubiquitin启动子，并对ZmASR1基因进行了生物信息学分析。序列分析结果表明，ZmASR1基因DNA全长为1 381 bp，包含1个长度为131 bp的内含子序列，存在1个完整的开放阅读框417 bp，编码138个氨基酸。氨基酸序列分析表明其具有ASR家族典型的保守结构域ABA_WDS(abscisic acid/water deficit stress)，氨基酸序列的N端有该家族成员所特有的依赖于Zn²⁺的DNA结合位点，而C端则有1个核定位信号，玉米的ZmASR1与单子叶植物的ZmASR1亲缘关系较近，而与双子叶植物的ZmASR1亲缘关系较远。并构建了同时带有该启动子和ZmASR1基因的植物表达载体。

Abstract:

-

参考文献/References:

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备注/Memo

备注/Memo:

收稿日期：2015-11-16
基金项目：国家公益性行业(农业)科研专项(编号：201403075)；中国热带农业科学院热带生物技术研究所基本科研业务费专项(编号：ITBB2015ZY14、ITBB2015ZD09)。
作者简介：张丽丽(1984—)，女，山东滨州人，博士，助理研究员，主要从事植物分子遗传学研究。E-mail：zhanglili@itbb.org.cn。
通信作者：易小平，博士，副研究员，主要从事作物遗传育种研究。Tel：(0898)66988690；E-mail：yixiaoping@itbb.org.cn。

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更新日期/Last Update: 2016-04-25