|本期目录/Table of Contents|

[1]罗岸.烟草合子时期特异表达基因的克隆与分析[J].江苏农业科学,2016,44(05):65-68.
 Luo An.Cloning and analysis of a specific expressed gene during zygote period of Nicotiana tabacum[J].Jiangsu Agricultural Sciences,2016,44(05):65-68.
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烟草合子时期特异表达基因的克隆与分析(PDF)
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第44卷
期数:
2016年05期
页码:
65-68
栏目:
生物技术
出版日期:
2016-05-25

文章信息/Info

Title:
Cloning and analysis of a specific expressed gene during zygote period of Nicotiana tabacum
作者:
罗岸
长江大学生命科学学院,湖北荆州 434023
Author(s):
Luo An
关键词:
烟草合子液泡基因克隆信号肽
Keywords:
-
分类号:
S572.01;Q785
DOI:
-
文献标志码:
A
摘要:
在烟草合子中克隆到1种新型的未知功能基因NtZE583,编码包含有91个氨基酸残基的多肽链。生物信息学分析显示:该蛋白的N端含有1段疏水性信号肽,但在其他物种中没有发现同类蛋白。NtZE583-绿色荧光蛋白(NtZE583-GFP)融合蛋白亚细胞定位显示,该蛋白可能存在于液泡中。利用染色体步移技术获得该基因5′端上游3 866 bp的侧翼序列(启动子和5′UTR),经检测发现,具有较强的启动子活性。研究结果为进一步研究该基因在烟草早期胚胎发生中的功能奠定了基础。
Abstract:
-

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:2015-04-29
基金项目:长江大学自然科学基金(编号:2014NSFY023)。
作者简介:罗岸(1984—),男,湖北荆州人,博士,讲师,研究方向为植物生殖发育。E-mail:Anluo@whu.edu.cn。
更新日期/Last Update: 2016-05-25