[1]龚静,林森,徐亚欧,等.泸宁鸡MyoD基因的克隆及生物信息学分析[J].江苏农业科学,2016,44(09):53-57.
Gong jing,et al.Cloning and bioinformatics analysis of MyoD gene from Luning chicken[J].Jiangsu Agricultural Sciences,2016,44(09):53-57.
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泸宁鸡MyoD基因的克隆及生物信息学分析(PDF)
Gong jing,et al.Cloning and bioinformatics analysis of MyoD gene from Luning chicken[J].Jiangsu Agricultural Sciences,2016,44(09):53-57.
《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]
- 卷:
- 第44卷
- 期数:
- 2016年09期
- 页码:
- 53-57
- 栏目:
- 生物技术
- 出版日期:
- 2016-09-25
文章信息/Info
- Title:
- Cloning and bioinformatics analysis of MyoD gene from Luning chicken
- Keywords:
- -
- 分类号:
- S831.2
- DOI:
- -
- 文献标志码:
- A
- 摘要:
- 为了研究MyoD基因在鸡生长发育与肉质形成中的作用，以泸宁鸡为研究对象，采用RT-PCR技术克隆MyoD基因序列，并利用生物信息学的方法，对其氨基酸同源性、理化性质等进行分析和预测。结果表明：泸宁鸡MyoD基因序列长度为988 bp，其编码的蛋白含299个氨基酸残基，具有碱性螺旋-环-螺旋结构(bHLH)；根据MyoD基因比对，构建的进化树显示泸宁鸡与人等哺乳动物的同源性为63%~67%，与原鸡、绿头鸭、游隼的同源性为 94%~99%。
- Abstract:
- -
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备注/Memo
- 备注/Memo:
-
收稿日期：2016-04-04
基金项目：四川省畜禽育种攻关项目(编号：2016NZ0099-36)。
作者简介：龚静(1974—)，男，四川渠县人，硕士，讲师，主要从事微生物分子生物学研究。E-mail：gongjing397@163.com。
通信作者：贺庆华(1977—)，男，四川彭州人，博士，副教授，主要从事分子生物学研究。E-mail：341400255@qq.com 。
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