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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:

第44卷

期数:

2016年11期

页码:

51-56

栏目:

生物技术

出版日期:

2016-11-25

文章信息/Info

Title:

Cloning and ethylene expression characteristic analysis of AfERF109 gene of Aechmea fasciata treated with exogenous ethylene

作者:

雷明;  王之;  王加宾;  李志英;  徐立

中国热带农业科学院热带作物品种资源研究所/农业部华南作物基因资源与种质创新重点实验室，海南儋州 571737

Author(s):

Lei Ming; et al

关键词:

蜻蜓凤梨; AfERF109; 克隆; 乙烯; 表达特性分析

Keywords:

-

分类号:

S668.301

DOI:

-

文献标志码:

A

摘要:

以热带观赏花卉蜻蜓凤梨(Aechemia fasciata)为材料，在分析蜻蜓凤梨转录组数据的基础上，结合cDNA末端快速扩增(rapid amplification of cDNA ends，简称RACE)技术克隆了APETALA2/乙烯反应元件结合蛋白(APETALA2/Ethylene Response Element Binding Protein，简称AP2/EREBP)家族的1个转录因子编码序列，并将其命名为AfERF109。AfERF109基因cDNA全长939 bp，开放阅读框(open reading frame，ORF)长762 bp，编码的蛋白含253个氨基酸残基。AfERF109分子量为28.275 1 ku，理论等电点为5.24；AfERF109不含信号肽和跨膜域，预测其定位于细胞核；AfERF109含有1个典型的AP2结构域，该结构域的二、三级结构构象保守；系统进化分析该蛋白分属ERF亚族的B-4类。实时荧光定量PCR分析表明，AfERF109转录本的表达量随着植株年龄的增长上调，在开花后的花柄中表达量最高。此外，AfERF109转录本的表达量受外源乙烯处理呈现正调控趋势。研究结果初步证实AfERF109参与乙烯调控路径，可能参与蜻蜓凤梨营养生长到生殖生长的时期转换及花柄的发育，为进一步研究AfERF109基因功能、通过基因工程手段调控蜻蜓凤梨开花提供了理论依据。

Abstract:

-

参考文献/References:

［1］Luther H E. An alphabetical list of bromeliad binomials［M］. Florida：The Bromeliad Society International，2010：4.
［2］Versieux L M，Barbara T，Wanderley M，et al. Molecular phylogenetics of the Brazilian giant bromeliads(Alcantarea，Bromeliaceae)：implications for morphological evolution and biogeography［J］. Molecular Phylogenetic Evolution，2012，64(1)：177-189.
［3］龚明霞，何铁光，黄如葵，等. 观赏凤梨组培快繁技术研究进展［J］. 广西农业科学，2010，41(5)：412-415.
［4］李志英. 凤梨科植物组织培养技术［M］. 北京：中国农业出版社，2014：1.
［5］Turnbull C G，Sinclair E R，Anderson K L，et al. Routes of ethephon uptake in pineapple(Ananas comosus)and reasons for failure of flower induction［J］. Journal of Plant Growth Regulation，1999，18(4)：145-152.
［6］Kuan C S，Yu C W，Lin M L，et al. Foliar application of aviglycine reduces natural flowering in pineapple［J］. HortScience，2005，40(1)：123-126.
［7］Dukovski D，Bernatzky R，Han S. Flowering induction of Guzmania by ethylene［J］. Scientia Horticulturae，2006，110(1)：104-108.
［8］Trusov Y，Botella J R. Silencing of the ACC synthase gene ACACS2 causes delayed flowering in pineapple［Ananas comosus (L.) Merr.］［J］. Journal of Experimental Botany，2006，57(14)：3953-3960.
［9］Müller M，Munné-Bosch S. Ethylene response factors：a key regulatory hub in hormone and stress signaling［J］. Plant Physiology，2015，169(1)：32-41.
［10］Shakeel S N，Gao Z，Amir M，et al. Ethylene regulates levels of ethylene receptor/CTR1 signaling complexes in Arabidopsis thaliana［J］. The Journal of Biological Chemistry，2015，290(19)：12415-12424.
［11］Kieber J J，Rothenberg M，Roman G，et al. CTR1，a negative regulator of the ethylene response pathway in Arabidopsis，encodes a member of the raf family of protein kinases［J］. Cell，1993，72(3)：427-441.
［12］Alonso J M，Hirayama T，Roman G，et al. EIN2，a bifunctional transducer of ethylene and stress responses in Arabidopsis［J］. Science，1999，284(5423)：2148-2152.
［13］Bleecker A B. Ethylene perception and signaling：an evolutionary perspective［J］. Trends in Plant Science，1999，4(7)：269-274.
［14］Chang C，Stadler R. Ethylene hormone receptor action in Arabidopsis［J］. BioEssays：News and Reviews in Molecular，Cellular and Developmental Biology，2001，23(7)：619-627.
［15］Solano R，Stepanova A，Chao Q，et al. Nuclear events in ethylene signaling：a transcriptional cascade mediated by ethylene-insensitive3 and ethylene-response-factor1［J］. Genes & Development，1998，12(23)：3703-3714.
［16］Wessler S R. Homing into the origin of the AP2 DNA binding domain［J］. Trends in Plant Science，2005，10(2)：54-56.
［17］Licausi F，Ohme-Takagi M，Perata P. APETALA2/Ethylene Responsive Factor(AP2/ERF)transcription factors：mediators of stress responses and developmental programs［J］. The New Phytologist，2013，199(3)：639-649.
［18］Ohta M，Matsui K，Hiratsu K，et al. Repression domains of class Ⅱ ERF transcriptional repressors share an essential motif for active repression［J］. The Plant Cell，2001，13(8)：1959-1968.
［19］Song C P，Agarwal M，Ohta M，et al. Role of an Arabidopsis AP2/EREBP-type transcriptional repressor in abscisic acid and drought stress responses［J］. The Plant Cell，2005，17(8)：2384-2396.
［20］Zhang G Y，Chen M，Chen X P，et al. Isolation and characterization of a novel EAR-motif-containing gene GmERF4 from soybean (Glycine max L.)［J］. Molecular Biology Reports，2009，37(2)：809-818.
［21］Zhang G Y，Chen M，Li L C，et al. Overexpression of the soybean GmERF3 gene，an AP2/ERF type transcription factor for increased tolerances to salt，drought，and diseases in transgenic tobacco［J］. Journal of Experimental Botany，2009，60(13)：3781-3796.
［22］Zhai Y，Li J W，Li X W，et al. Isolation and characterization of a novel transcriptional repressor GmERF6 from soybean［J］. Biologia Plantarum，2012，57(1)：26-32.
［23］Ohme-Takagi M，Shinshi H. Ethylene-inducible DNA binding proteins that interact with an ethylene-responsive element［J］. The Plant Cell，1995，7(2)：173-182.
［24］Yi S Y，Kim J H，Joung Y H，et al. The pepper transcription factor CaPF1 confers pathogen and freezing tolerance in Arabidopsis［J］. Plant Physiology，2004，136(1)：2862-2874.
［25］McGrath K C. Repressor-and activator-type ethylene response factors functioning in jasmonate signaling and disease resistance identified via a genome-wide screen of Arabidopsis transcription factor gene expression［J］. Plant Physiology，2005，139(2)：949-959.
［26］Zuo K J，Qin J，Zhao J Y，et al. Over-expression GbERF2 transcription factor in tobacco enhances brown spots disease resistance by activating expression of downstream genes［J］. Gene，2007，391(1/2)：80-90.
［27］Cheng M C，Liao P M，Kuo W W，et al. The Arabidopsis ethylene response factor1 regulates abiotic stress-responsive gene expression by binding to different cis-acting elements in response to different stress signals［J］. Plant Physiology，2013，162(3)：1566-1582.
［28］Li Z Y，Wang J B，Zhang X Q，et al. Transcriptome sequencing determined flowering pathway genes in Aechmea fasciata treated with ethylene［J］. Journal of Plant Growth Regulation，2016，35(2)：316-329.
［29］Lei M，Li Z Y，Wang J B，et al. AfAP2-1，an age-dependent gene of Aechmea fasciata，responds to exogenous ethylene treatment［J］. International Journal of Molecular Sciences，2016，17(3)：303.
［30］丛汉卿，信彩云，张银东，等. ‘阿蒂擎天’凤梨谷胱甘肽-S-转移酶基因的克隆与乙烯诱导表达特性的初步分析［J］. 分子植物育种，2013，11(3)：365-370.
［31］Nakano T，Suzuki K，Fujimura T，et al. Genome-wide analysis of the ERF gene family in Arabidopsis and rice［J］. Plant Physiology，2006，140(2)：411-432.
［32］Zhang G，Chen M，Chen X，et al. Phylogeny，gene structures，and expression patterns of the ERF gene family in soybean(Glycine max L.)［J］. Journal of Experimental Botany，2008，59(15)：4095-4107.
［33］Wang X P，Liu S，Tian H N，et al. The small ethylene response factor ERF96 is involved in the regulation of the abscisic acid response in Arabidopsis［J］. Frontiers in Plant Science，2015，6：1064-1076.
［34］Tuan P A，Bai S L，Saito T，et al. Involvement of EARLY BUD-BREAK，an AP2/ERF transcription factor gene，in bud break in Japanese pear(Pyrus pyrifolia Nakai)lateral flower buds：expression，histone modifications，and possible target genes［J］. Plant and Cell Physiology，2016，57(5)：1038-1047.
［35］Wisniewski M，Norelli J，Artlip T. Overexpression of a peach CBF gene in apple：a model for understanding the integration of growth，dormancy，and cold hardiness in woody plants［J］. Frontiers in Plant Science，2015，6：85.
［36］Busov V，Carneros E，Yakovlev I. EARLY BUD-BREAK1(EBB1)defines a conserved mechanism for control of bud-break in woody perennials［J］. Plant Signaling & Behavior，2016，11(2)：e1073873.
［37］Liu J X，Li J Y，Wang H N，et al. Identification and expression analysis of ERF transcription factor genes in petunia during flower senescence and in response to hormone treatments［J］. Journal of Experimental Botany，2011，62(2)：825-840.
［38］Yu Y W，Yang D X，Zhou S R，et al. The ethylene response factor OsERF109 negatively affects ethylene biosynthesis and drought tolerance in rice［J］. Protoplasma，2016：1-8.
［39］Zhang H W，Zhang J F，Quan R D，et al. EAR motif mutation of rice OsERF3 alters the regulation of ethylene biosynthesis and drought tolerance［J］. Planta，2013，237(6)：1443-1451.

相似文献/References:

备注/Memo

备注/Memo:

收稿日期：2016-05-18
基金项目：国家自然科学基金面上项目(编号：31372106)；海南省自然科学基金面上项目(编号：20163127)。
作者简介：雷明(1982—)，男，安徽池州人，博士，助理研究员，主要从事凤梨科植物开花和光合分子机理研究。Tel：(0898)23300284；E-mail：leiming_catas@126.com。
通信作者：徐立，博士，研究员，主要从事凤梨、香蕉等热带作物生长发育调控机理研究。Tel：(0898)23300284；E-mail：xllzy@263.net。

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更新日期/Last Update: 2016-11-25