|本期目录/Table of Contents|

[1]宋慧,王毓洪,张香琴.瓠瓜类型砧木遗传作图群体双亲多态性分析[J].江苏农业科学,2016,44(11):48-50.
 Song Hui,et al.Analysis of parental polymorphism of genetic mapping populations of bottle gourd rootstock[J].Jiangsu Agricultural Sciences,2016,44(11):48-50.
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瓠瓜类型砧木遗传作图群体双亲多态性分析(PDF)
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第44卷
期数:
2016年11期
页码:
48-50
栏目:
生物技术
出版日期:
2016-11-25

文章信息/Info

Title:
Analysis of parental polymorphism of genetic mapping populations of bottle gourd rootstock
作者:
宋慧 王毓洪 张香琴
宁波市农业科学研究院蔬菜研究所/宁波市瓜菜育种重点实验室,浙江宁波 315040
Author(s):
Song Huiet al
关键词:
瓠瓜砧木多态性分析耐湿涝
Keywords:
-
分类号:
S184
DOI:
-
文献标志码:
A
摘要:
以耐湿涝瓠瓜类型砧木材料JZS与不耐湿涝材料T2002为遗传作图双亲,利用随机扩增多态性DNA(random amplification polymorphic DNA,简称RAPD)法与瓠瓜及其他近源瓜类简单重复序列(simple sequence repeat,简称SSR)进行多态性分析。从供试1 678个RAPD、SSR标记中共筛选到26个多态性引物,其中包括14个RAPD、4个瓠瓜SSR、2个甜瓜SSR、5个黄瓜SSR、1个南瓜SSR,扩增出28个多态性位点。几种标记多态性检测效率不同,瓠瓜SSR引物揭示,双亲多态性比例最高(0.47%),其次是RAPD(0.38%),近源瓜类SSR选择效率最低,仅有0.14%~015%。结果表明,尽管瓠瓜作图亲本表型差异大,但分子标记结果显示亲本遗传背景相近;甜瓜、黄瓜和南瓜与瓠瓜亲缘关系远,具有种属特异性的SSR引物在瓠瓜亲本JZS、T2002多态性分析中通用性不高。为了提高分子标记多态性分析效率,绘制高密度遗传图谱,下一步应尽快开展基于基因组测序技术检测单核苷酸多态性(single nucleotide polymorphism,简称SNP)位点的研究。
Abstract:
-

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:2015-10-16
基金项目:宁波市自然科学基金(编号:2014A610199);公益性行业(农业)科研专项(编号:201403032)。
作者简介:宋慧(1979—),女,甘肃兰州人,博士,副研究员,主要从事蔬菜分子辅助育种与生物工程研究。Tel:(0574)87923251;E-mail:975281674@qq.com。
更新日期/Last Update: 2016-11-25