|本期目录/Table of Contents|

[1]韩雪,朱丽莉,粟朝芝,等.长顺绿壳蛋鸡PRL基因的克隆及序列分析[J].江苏农业科学,2016,44(12):82-85.
 Han Xue,et al.Cloning and sequence analysis of prolactin gene from Changshun blue-eggshell chicken[J].Jiangsu Agricultural Sciences,2016,44(12):82-85.
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长顺绿壳蛋鸡PRL基因的克隆及序列分析(PDF)
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第44卷
期数:
2016年12期
页码:
82-85
栏目:
生物技术
出版日期:
2016-12-25

文章信息/Info

Title:
Cloning and sequence analysis of prolactin gene from Changshun blue-eggshell chicken
作者:
韩雪朱丽莉粟朝芝吴松成陶宇航
贵州省畜牧兽医研究所,贵州贵阳 550005
Author(s):
Han Xueet al
关键词:
长顺绿壳蛋鸡催乳素基因克隆序列分析
Keywords:
-
分类号:
Q785
DOI:
-
文献标志码:
A
摘要:
采用特异性引物PCR技术获得长顺绿壳蛋鸡PRL基因,将其克隆至pGM-T载体,并对该序列进行测序分析。结果表明,长顺绿壳蛋鸡PRL基因全序列长度为690 bp,包含5个外显子和4个内含子,编码229个氨基酸。将长顺绿壳蛋鸡PRL基因与GenBank已公布的鸡、鸭、鹅、鱼等动物的PRL基因序列进行同源性比较,发现亲缘关系越远则同源性越低。
Abstract:
-

参考文献/References:

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[9]陈兴勇,何艳,时丹,等. 皖西白鹅催乳素基因克隆、表达及主动免疫后就巢行为分析[J]. 扬州大学学报:农业与生命科学版,2014,35(2):33-37.
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备注/Memo

备注/Memo:
收稿日期:2015-10-13
基金项目:贵州省科学技术基金(编号:黔科合J字[2011]2174);贵州省农业科学院研究生创新基金(编号:黔农科合[创新基金]2010011);贵州省动植物育种专项(编号:黔农育专字[2011]027);贵州省农业科学院专项(编号:黔农科院院专项[2014]006号)。
作者简介:韩雪(1982—),女,江苏徐州人,硕士,助理研究员,主要从事动物遗传育种研究。E-mail:hanxue8855601@126.com。
通信作者:陶宇航,副研究员,主要从事家禽养殖研究。E-mail:1043694421@qq.com。
更新日期/Last Update: 2016-12-25