|本期目录/Table of Contents|

[1]薛涛涛,边金,伍陆,等.酿酒酵母组蛋白H3K56A突变菌株的构建及其对细胞生长的影响[J].江苏农业科学,2016,44(12):77-81.
　Xue Taotao,et al.Construction of histone H3K56A mutant of Saccharomyces cerevisiae and its effects on cell growth[J].Jiangsu Agricultural Sciences,2016,44(12):77-81.
点击复制

酿酒酵母组蛋白H3K56A突变菌株的构建
及其对细胞生长的影响(PDF)
分享到：

《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第44卷
期数:
2016年12期
页码:
77-81
栏目:
生物技术
出版日期:
2016-12-25

文章信息/Info

Title:
Construction of histone H3K56A mutant of Saccharomyces cerevisiae and its effects on cell growth
作者:
薛涛涛; 边金; 伍陆; 赵秀娟; 崔向军; 蔡禄
内蒙古科技大学数理与生物工程学院，内蒙古包头 014010
Author(s):
Xue Taotao; et al
关键词:
酿酒酵母; H3K56A突变菌; 组蛋白修饰; 两步替换法; 同源重组; 高盐环境; 高温环境; 抗逆机制
Keywords:
-
分类号:
S188
DOI:
-
文献标志码:
A
摘要:
组蛋白修饰，例如甲基化、乙酰化等修饰对基因表达和细胞生长至关重要。为揭示组蛋白H3第56位赖氨酸(K)修饰对酵母细胞生长的重要性，构建H3K56定点突变为丙氨酸(A)的组蛋白突变株H3K56A，并对其在高温、高盐等条件下的生长状况进行初步检测。根据酵母同源重组的原理，采用两步替换法构建H3K56A突变菌株，用分光光度法测定突变菌株的生长曲线，并分别在高盐、高温条件下检测突变体的表型。结果表明，酿酒酵母组蛋白H3K56A突变菌株构建成功，其生长曲线与野生型无明显差异；H3K56A突变株对高温、高盐条件均较敏感，且在高盐条件下，菌体生长缓慢、菌落偏小，表明组蛋白H3K56位点的修饰可能与酿酒酵母抗高盐、高温机制有关。
Abstract:
-

参考文献/References:

［1］Jiang C，Pugh B F. Nucleosome positioning and gene regulation：advances through genomics［J］. Nature Reviews Genetics，2009，10(3)：161-172.
［2］Thoma F，Koller T，Klug A. Involvement of histone H1 in the organization of the nucleosome and of the salt-dependent superstructures of chromatin［J］. Journal of Cell Biology，1979，83：403-427.
［3］周蔚，王世鑫. 组蛋白修饰与基因调控［J］. 国外医学：分子生物学分册，2003，25(2)：71-73.
［4］Smith M M，Murray K. Yeast H3 and H4 histone messenger RNAs are transcribed from two non-allelic gene sets［J］. Journal of Molecular Biology，1983，169(3)：641-661.
［5］Smith M M，Stirling V B. Histone H3 and H4 gene deletions in Saccharomyces cerevisiae［J］. Journal of Cell Biology，1988，106(3)：557-566.
［6］Hereford L，Fahrner K，Woolford J，et al. Isolation of yeast histone genes H2A and H2B［J］. Cell，1979，18(4)：1261-1271.
［7］Ng H H，Feng Q，Wang H B，et al. Lysine methylation within the globular domain of histone H3 by Dot1 is important for telomeric silencing and Sir protein association［J］. Genes & Development，2002，16(12)：1518-1527.
［8］蒋智文，刘新光，周中军. 组蛋白修饰调节机制的研究进展［J］. 生物化学与生物物理进展，2009，36(10)：1252-1259.
［9］Kouzarides T. Chromatin modifications and their function［J］. Cell，2007，128(4)：693-705.
［10］Chen H，Fan M，Pfeffer L M，et al. The histone H3 lysine 56 acetylation pathway is regulated by target of rapamycin (TOR) signaling and functions directly in ribosomal RNA biogenesis［J］. Nucleic Acids Research，2012，40(14)：6534-6546.
［11］Faulk C，Dolinoy D C. Timing is everything The when and how of environmentally induced changes in the epigenome of animals［J］. Epigenetics，2011，6(7)：791-797.
［12］Vaquero A，Reinberg D. Calorie restriction and the exercise of chromatin［J］. Genes & Development，2009，23(16)：1849-1869.
［13］Dai J B，Hyland E M，Yuan D S，et al. Probing nucleosome function：A highly versatile library of synthetic histone H3 and H4 mutants［J］. Cell，2008，134(6)：1066-1078.
［14］Gietz R D，Schiestl R H，Willems A R，et al. Studies on the transformation of intact yeast cells by the LiAc/SS-DNA/PEG procedure［J］. Yeast，1995，11(4)：355-360.
［15］Sterner D E，Berger S L. Acetylation of histones and transcription-related factors［J］. Microbiology and Molecular Biology Reviews，2000，64(64)：435-459.
［16］Williams S K，Truong D，Tyler J K. Acetylation in the globular core of histone H3 on lysine-56 promotes chromatin disassembly during transcriptional activation［J］. Proceedings of the National Academy of Sciences of the United States of America，2008，105(26)：9000-9005.
［17］Recht J，Tsubota T，Tansy J C，et al. Histone chaperone Asf1 is required for histone H3 lysine 56 acetylation，a modification associated with S phase in mitosis and meiosis［J］. Proceedings of the National Academy of Sciences of the United States of America，2006，103(18)：6988-6993.
［18］Morano K A，Grant C M，Moye-Rowley W S. The response to heat shock and oxidative stress in saccharomyces cerevisiae［J］. Genetics，2012，190(4)：1157-1195.
［19］Akerfelt M，Morimoto R I，Sistonen L. Heat shock factors：integrators of cell stress，development and lifespan［J］. Nature Reviews Molecular Cell Biology，2010，11(8)：545-555.
［20］Yamamoto N，Maeda Y，Ikeda A，et al. Regulation of thermotolerance by stress-induced transcription factors in Saccharomyces cerevisiae［J］. Eukaryotic Cell，2008，7(5)：783-790.

相似文献/References:

[1]王震,刘标,陈薇,等.养殖污水中氨氮降解菌的分离、筛选及鉴定[J].江苏农业科学,2013,41(08):377.
　Wang Zhen,et al.Isolation，screening and identification of ammonia nitrogen degradation strains from aquaculture sewage[J].Jiangsu Agricultural Sciences,2013,41(12):377.
[2]王珊珊,徐海洋,王世明.酵母穿梭质粒pXZ200的构建[J].江苏农业科学,2015,43(08):31.
　Wang Shanshan,et al.Construction of yeast shuttle plasmid pXZ200[J].Jiangsu Agricultural Sciences,2015,43(12):31.
[3]费文斌,雍晓雨,徐俊,等.1株耐热产乙醇酵母的分离、鉴定与性能测试[J].江苏农业科学,2015,43(03):319.
　Fei Wenbin,et al.Separation，identification and performance test of a strain of heat-resisting and alcohol producing yeast[J].Jiangsu Agricultural Sciences,2015,43(12):319.
[4]叶云,付军鹏,李祎予,等.紫檀芪处理对酿酒酵母基因组表达变化的影响[J].江苏农业科学,2017,45(05):44.
　Ye Yun,et al.Effect of pterostilbene processing on genome expression of Saccharomyces cerevisiae[J].Jiangsu Agricultural Sciences,2017,45(12):44.
[5]张传丽,高明侠,董玉玮,等.乙醛脱氢酶基因表达载体的构建及表达条件筛选[J].江苏农业科学,2018,46(07):40.
　Zhang Chuanli,et al.Construction of expression vector and screening of expression conditions of acetaldehyde dehydrogenase gene[J].Jiangsu Agricultural Sciences,2018,46(12):40.
[6]谢鹏,袁园,葛莹,等.酵母菌发酵对常用饲料原料营养指标和抗营养因子含量的影响[J].江苏农业科学,2019,47(21):241.
　Xie Peng,et al.Effects of yeast fermentation on nutritive parameters and anti-nutritive factor contents in common feed stuff[J].Jiangsu Agricultural Sciences,2019,47(12):241.
[7]韦梦婷,王英,刘小莉,等.低度黑莓果酒发酵菌株筛选与工艺优化[J].江苏农业科学,2021,49(3):156.
　Wei Mengting,et al.Screening of fermentation strains and optimization of process for low-alcohol blackberry wine[J].Jiangsu Agricultural Sciences,2021,49(12):156.

备注/Memo

备注/Memo:
收稿日期：2015-11-02
基金项目：国家自然科学基金(编号：61361014、31260274)；内蒙古自然科学基金(编号：2015MS0334)。
作者简介：薛涛涛(1990—)，女，山西临县人，硕士研究生，主要从事表观遗传学研究。E-mail：xuetaotao196485@163.com。
通信作者：赵秀娟，博士，教授，主要从事表观遗传学研究。E-mail：zhaoxiujuan@imust.cn。
更新日期/Last Update: 2016-12-25