|本期目录/Table of Contents|

[1]王婧.荷花MADS-box基因的克隆及表达分析[J].江苏农业科学,2017,45(01):39-42.
 Wang Jing.Cloning and expression analysis of a flowering-related MADS-box gene in lotus (Nelumbo nucifera Gaertn.)[J].Jiangsu Agricultural Sciences,2017,45(01):39-42.
点击复制

荷花MADS-box基因的克隆及表达分析(PDF)
分享到:

《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第45卷
期数:
2017年01期
页码:
39-42
栏目:
生物技术
出版日期:
2017-01-05

文章信息/Info

Title:
Cloning and expression analysis of a flowering-related MADS-box gene in lotus (Nelumbo nucifera Gaertn.)
作者:
王婧
吉林省经济管理干部学院,吉林长春 130012
Author(s):
Wang Jing
关键词:
荷花花发育MADS-box基因表达RT-PCR
Keywords:
-
分类号:
S682.320.1;Q785
DOI:
-
文献标志码:
A
摘要:
采用RT-PCR方法从微山红莲花苞中克隆获得1个与花发育相关的MADS-box基因,命名为NeMADS1。该基因全长729 bp,包含1个720 bp的开放阅读框,编码239个氨基酸,具有典型的MADS结构域和K结构域。序列比对和系统进化分析结果表明,NeMADS1与E类家族AGL9/SEP-like3基因亲缘关系较近。RT-PCR分析结果表明,NeMADS1基因在花瓣、雄蕊和心皮中表达。
Abstract:
-

参考文献/References:

[1]Weigel D,Nilsson O.A developmental switch sufficient for flower initiation in diverse plants[J]. Nature,1995,377(6549):495-500.
[2]Alvarezbuylla E R,Liljegren S J,Pelaz S,et al. MADS-box gene evolution beyond flowers: expression in pollen,endosperm,guard cells,roots and trichomes.[J]. Plant Journal for Cell & Molecular Biology,2000,24(4):457-66.
[3]Buchner P,Boutin J-P.A MADS box transcription factor of the AP1/AGL9 subfamily is also expressed in the seed coat of pea (Pisum sativum) during development[J]. Plant Molecular Biology,1998,38(6):1253-1255.
[4]Gu Q,Ferrándiz C,Yanofsky M F,et al.The FRUITFULL MADS-box gene mediates cell differentiation during Arabidopsis fruit development[J]. Development,1998,125(8):1509-1517.
[5]Coen E S,Meyerowitz E M.The war of the whorls: genetic interactions controlling flower development[J]. Nature,1991,353(6339):31-37.
[6]Honma T,Goto K.Complexes of MADS-box proteins are sufficient to convert leaves into floral organs[J]. Nature,2001,409(6819):525-529.
[7]Jack T.Molecular and genetic mechanisms of floral control[J]. The Plant Cell Online,2004,16(S1):S1-S17.
[8]Pelaz S,Ditta G S,Baumann E,et al.B and C floral organ identity functions require SEPALLATA MADS-box genes[J]. Nature,2000,405(6783):200-203.
[9]Theissen G,Saedler H.Plant biology: floral quartets[J]. Nature,2001,409(6819):469-471.
[10]Colombo L,Franken J,Koetje E,et al. The petunia MADS box gene FBP11 determines ovule identity[J]. The Plant Cell,1995,7(11):1859-1868.
[11]郭爽,马宁,杨文才,等. 辣椒花器官发育MADS-box基因的克隆与表达分析[J]. 园艺学报,2010,37(10):1591-1597.
[12]Huang H,Tudor M,Weiss C A,et al.The Arabidopsis MADS-box gene AGL3 is widely expressed and encodes a sequence-specific DNA-binding protein[J]. Plant Molecular Biology,1995,28(3):549-567.
[13]Ma H,Yanofsky M F,Meyerowitz E M.AGL1-AGL6,an Arabidopsis gene family with similarity to floral homeotic and transcription factor genes[J]. Genes & Development,1991,5(3):484-495.
[14]Mandel M,Yanofsky M F.The Arabidopsis AGL9 MADS box gene is expressed in young flower primordia[J]. Sexual Plant Reproduction,1998,11(1):22-28.
[15]Zahn L M,Kong H,Leebens-Mack J H,et al. The evolution of the SEPALLATA subfamily of MADS-Box genes:a preangiosperm origin with multiple duplications throughout angiosperm history[J]. Genetics,2005,169(4):2209-2223.
[16]Ditta G,Pinyopich A,Robles P,et al. The SEP4 gene of Arabidopsis thaliana functions in floral organ and meristem identity[J]. Current Biology,2004,14(21):1935-1940.
[17]Tani E,Polidoros A N,Flemetakis E,et al. Characterization and expression analysis of AGAMOUS-like,SEEDSTICK-like,and SEPALLATA-like MADS-box genes in peach (Prunus persica) fruit[J]. Plant Physiology and Biochemistry,2009,47(8):690-700.
[18]Ireland H S,Yao J L,Tomes S,et al.Apple SEPALLATA1/2-like genes control fruit flesh development and ripening[J]. The Plant Journal,2013,73(6):1044-1056.
[19]Seymour G B,Ryder C D,Cevik V,et al.A SEPALLATA gene is involved in the development and ripening of strawberry (Fragaria×ananassa Duch.) fruit,a non-climacteric tissue [J]. Journal of Experimental Botany,2011,62(3):1179-1188.
[20]Chunlei J,Lin X,Dehui Q,et al.Cloning and expression analysis of CgSEP3 gene from Cymbidium goeringii[J]. Acta Botanica Boreali-Occidentalia Sinica,2014.
[21]Chang Y,Chiu Y,Wu J,et al.Four orchid (Oncidium Gower Ramsey)AP1/AGL9-like MADS box genes show novel expression patterns and cause different effects on floral transition and formation in Arabidopsis thaliana[J]. Plant and Cell Physiology,2009,50(8):1425-1438.
[22]Purugganan M D,Rounsley S D,Schmidt R J,et al.Molecular evolution of flower development: diversification of the plant MADS-box regulatory gene family[J]. Genetics,1995,140(1):345-356.
[23]Vandenbussche M,Theissen G,Van de Peer Y,et al.Structural diversification and neo-functionalization during floral MADS-box gene evolution by C-terminal frameshift mutations[J]. Nucleic Acids Research,2003,31(15):4401-4409.
[24]徐启江,关录飞,吴笑女,等. 草原龙胆MADS-box基因的克隆及表达分析[J]. 植物学报,2008,25(4):415-429.
[25]Malcomber S T,Kellogg E A.SEPALLATA gene diversification: brave new whorls[J]. Trends in Plant Science,2005,10(9):427-435.

相似文献/References:

[1]徐君,李静会,李欣,等.荷花ISSR引物筛选及反应体系优化[J].江苏农业科学,2014,42(10):42.
 Xu Jun,et al.Screening of lotus ISSR primers and optimization of its reaction system[J].Jiangsu Agricultural Sciences,2014,42(01):42.
[2]姜红卫,李欣,江君,等.苏南观赏荷花设施栽培周年开花技术研究[J].江苏农业科学,2013,41(12):191.
 Jiang Hongwei,et al.Study on anniversary bloom of ornamental lotus in greenhouse of southern Jiangsu[J].Jiangsu Agricultural Sciences,2013,41(01):191.
[3]徐君,李欣,江君,等.不同花色荷花色素成分及稳定性分析[J].江苏农业科学,2016,44(02):331.
 Xu Jun,et al.nalysis of ingredients and stability of flower pigment of Nelumbo nucifera cultivars with different colors[J].Jiangsu Agricultural Sciences,2016,44(01):331.
[4]桂腾琴,伍伟,卢鹏,等.正交设计优化荷花品种ISSR-PCR反应体系[J].江苏农业科学,2016,44(07):71.
 Gui Tengqin,et al.Optimization of reaction system of Nelumbo nucifera Gaertn using orthogonal design[J].Jiangsu Agricultural Sciences,2016,44(01):71.
[5]江君,李欣,徐飞,等.3个荷花品种对富营养水体和底泥中氮、磷去除能力比较研究[J].江苏农业科学,2018,46(08):296.
 Jiang Jun,et al.Comparison of removal nitrogen and phosphorus capacity from eutrophic water and sediment among three cultivars of Nelumbo nucifera[J].Jiangsu Agricultural Sciences,2018,46(01):296.
[6]胡鑫,王彦杰,金奇江,等.活体取样并鉴定荷花花芽分化时期的方法研究[J].江苏农业科学,2018,46(24):113.
 Hu Xin,et al.Study on methods of sampling in vivo and flower bud differentiation period identification of Nelumbo nucifera[J].Jiangsu Agricultural Sciences,2018,46(01):113.
[7]徐君,江君,王欢,等.荷花淹水胁迫下实时定量PCR内参基因的验证[J].江苏农业科学,2019,47(17):50.
 Xu Jun,et al.Validation of reference genes for quantitative real-time PCR in lotus (Nelumbo nucifera) under submergence treatment[J].Jiangsu Agricultural Sciences,2019,47(01):50.
[8]刘光杨,周 炜,白竹谊,等.耐阴小型荷花品种筛选研究[J].江苏农业科学,2020,48(10):186.
 Liu Guangyang,et al.Screening of Nelumbo nucifera cultivars with shade tolerance[J].Jiangsu Agricultural Sciences,2020,48(01):186.
[9]原鑫,李文玲,刘召强,等.荷花品种表型性状遗传多样性分析[J].江苏农业科学,2020,48(16):188.
 Yuan Xin,et al.Genetic diversity analysis of phenotypic traits of lotus varieties[J].Jiangsu Agricultural Sciences,2020,48(01):188.
[10]李心,杨柳燕,顾俊杰,等.红掌花芽分化进程及其主要生理指标变化研究[J].江苏农业科学,2020,48(24):140.
 Li Xin,et al.Study on flower bud differentiation process and major physiological indices changes of Anthurium andraeanum[J].Jiangsu Agricultural Sciences,2020,48(01):140.

备注/Memo

备注/Memo:
收稿日期:2015-11-26
作者简介:王婧(1981—),女,黑龙江哈尔滨人,硕士,讲师,主要从事园林植物栽植及分子育种方面研究。E-mail:47292634@qq.com。
更新日期/Last Update: 2017-01-05