|本期目录/Table of Contents|

[1]张丹丹,刘廷辉,赵丹,等.球孢白僵菌胞外酶的活性与其毒力的相关性[J].江苏农业科学,2017,45(02):93-95.
 Zhang Dandan,et al.Correlation between extracellular enzyme activity of Beauveria bassiana and virulence[J].Jiangsu Agricultural Sciences,2017,45(02):93-95.
点击复制

球孢白僵菌胞外酶的活性与其毒力的相关性(PDF)
分享到:

《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第45卷
期数:
2017年02期
页码:
93-95
栏目:
植物保护
出版日期:
2017-01-20

文章信息/Info

Title:
Correlation between extracellular enzyme activity of Beauveria bassiana and virulence
作者:
张丹丹 刘廷辉 赵丹 李瑞军 董建臻 陆秀君
河北农业大学植物保护学院,河北保定 071001
Author(s):
Zhang Dandanet al
关键词:
球孢白僵菌胞外酶毒力相关性
Keywords:
-
分类号:
S432.4+4
DOI:
-
文献标志码:
A
摘要:
为了研究球孢白僵菌胞外酶的活性与其毒力的相关性,以黄粉虫为标准试虫测定9株球孢白僵菌的毒力,并分别测定其胞外蛋白酶、几丁质酶和脂肪酶的生产水平。相关分析表明,3种胞外酶活性与毒力的线性关系分别为y1=-13.815 4x+185.954 4,r12=0.495 7;y2=-10.976 0x+58.977 0,r22=0.432 5;y3=-1.122 3x+8.469 5,r32=0.830 3。由r2值可以看出,胞外脂肪酶活性与毒力的线性关系最明显,脂肪酶活性可作为大量菌株毒力初筛的参考性指标。
Abstract:
-

参考文献/References:

[1]林海萍,韩正敏,张昕,等. 球孢白僵菌研究现状及提高其杀虫效果展望[J]. 浙江农林大学学报,2006,23(5):575-580.
[2]刘廷辉,武丽芬,李瑞军,等. 7株白僵菌菌株的抗逆性研究[J]. 河北农业大学学报,2013,36(3):82-86,97.
[3]李增智,李春如,黄勃. 中国虫生真菌研究与应用[J]. 昆虫知识,1988,1:241-255.
[4]Tafoya F,Zunigadelgadillo M,Alatorre R,et al. Pathogenicity of Beauveria bassiana (Deuteromycota:Hyphomycetes) against the cactus weevil,Metamasius spinolae (Coleoptera:Curculionidae) under laboratory conditions[J]. Florida Entomologist,2004,87(4):533-536.
[5]李阜棣,胡正嘉. 微生物学[M]. 北京:中国农业出版社,2000:283-284.
[6]蒲蛰龙. 害虫生物防治[M]. 北京:科学出版社,1977:104-108.
[7]王园园. 球孢白僵菌分生孢子田间生存潜能及与常用药剂的相容性研究[D]. 保定:河北农业大学,2013.
[8]季香云,杨长举. 白僵菌的致病性与应用[J]. 中国生物防治学报,2003,19(2):82-85.
[9]Stleger R J,Bidochka M J,Roberts D W. Isoforms of the cuticle-degrading Pr1 proteinase and production of a metalloproteinase by Metarhizium anisopliae[J]. Archives of Biochemistry and Biophysics,1994,313(1):1-7.
[10] Leger R J,Frank D C,Roberts D W,et al. Molecular cloning and regulatory analysis of the cuticle-degrading-protease structural gene from the entomopathogenic fungus Metarhizium anisopliae[J]. European Journal of Biochemistry,1992,204(3):991-1001.
[11]Fang W,Leng B,Xiao Y,et al. Cloning of Beauveria bassiana chitinase gene Bbchit1 and its application to improve fungal strain virulence[J]. Applied and Environmental Microbiology,2005,71(1):363-370.
[12]St. Leger R J. The role of cuticle-degrading proteases in fungal pathogenesis of insects[J]. Canadian Journal of Botany,1995,73(S1):1119-1125.
[13]樊美珍,胡景江. 白僵菌酯酶同工酶、脂肪酸与其毒力的关系[J]. 安徽农业大学学报,1996,23(3):260-266.
[14]姚剑,黄大庆. 球孢白僵菌蛋白质酶、几丁质酶和β-N-乙酰葡萄糖苷酶产生水平及其与毒力关系的研究[J]. 宿州学院学报,2004,19(4):102-106.
[15]冯明光. 胞外蛋白酶和脂酶活性作为球孢白僵菌毒力指标的可靠性分析[J]. 微生物学报,1998,38(6):461-467.
[16]Pavlyushin V A. Virulence mechanisms of the entomopathogenic fungus Beauveria bassiana (Bals.) Vuill[C]//Proceedings of the First Joint US USSR Conference on the Production,Selection and Standardization of Entomopathogenic Fungi of US/USSR Joint Working Group on the Production of Substances by Microbiological Means,1978.
[17]Smith R J,Pekrul S,Grula E A. Requirement for sequential enzymatic activities for penetration of the integument of the corn earworm (Heliothis zea)[J]. Journal of Invertebrate Pathology,1981,38(3):335-344.

相似文献/References:

[1]滕忠才,张立红,刘廷辉,等.小菜蛾高毒力球孢白僵菌菌株抗旱性研究[J].江苏农业科学,2013,41(09):119.
 Teng Zhongcai,et al.Study on drought resistance of Beauveria bassiana with high virulence on Plutella xylostella[J].Jiangsu Agricultural Sciences,2013,41(02):119.
[2]袁盛勇,孔琼,孙燕,等.球孢白僵菌对南瓜实蝇致病力的测定[J].江苏农业科学,2015,43(09):158.
 Yuan Shengyong,et al.Susceptibility of Bactrocera tau (Walker) to Beauveria bassiana Vuillemin[J].Jiangsu Agricultural Sciences,2015,43(02):158.
[3]李红,杨镇,吕立涛,等.黑木耳栽培过程中抗霉能力及胞外酶活性变化[J].江苏农业科学,2017,45(10):109.
 Li Hong,et al.Changes of antifungal ability and extracellular enzyme activity during cultivation of Auricularia auricula[J].Jiangsu Agricultural Sciences,2017,45(02):109.
[4]陶淑霞,薛丹,肖悦,等.黄粉虫虫粉对球孢白僵菌孢子粉产量、质量及其毒力的影响[J].江苏农业科学,2017,45(22):114.
 Tao Shuxia,et al.Effects of pupa powder of Tenebrio molitor on conidial yield,quality,and virulence of Beauveria bassiana[J].Jiangsu Agricultural Sciences,2017,45(02):114.
[5]陶淑霞,陆子慧.大豆食心虫高毒力球孢白僵菌菌株的筛选[J].江苏农业科学,2018,46(05):91.
 Tao Shuxia,et al.Screening of high virulent strains of Beauveria bassiana against Leguminivora glycinivorella[J].Jiangsu Agricultural Sciences,2018,46(02):91.
[6]刘欣怡,陈敏,雷雅婷,等.新鲜巨菌草对平菇胞外酶活性以及栽培料营养成分变化的影响[J].江苏农业科学,2020,48(2):159.
 Liu Xinyi,et al.The effects of fresh Pennisetum sinese on extracellular enzyme activity of Pleurotus ostreatus and nutrient composition changes of cultivated materials[J].Jiangsu Agricultural Sciences,2020,48(02):159.
[7]王清,张勇跃,孟凡奇,等.hpaXm和hpaXm-IP基因对棉花角斑病菌V8表型及HR的影响[J].江苏农业科学,2020,48(8):116.
 Wang Qing,et al.Effects of hpaXm and hpaXm-IP genes on phenotype and hypersensitive response of cotton leaf spot pathogen V8[J].Jiangsu Agricultural Sciences,2020,48(02):116.
[8]赵芳娟,邓百万,解修超,等.耐高温萌发菌胞外酶活性与天麻种子萌发的关系[J].江苏农业科学,2022,50(4):205.
 Zhao Fangjuan,et al.Relationship between extracellular enzyme activity of heat-resistant germination bacteria and germination of Gastrodia elata seeds[J].Jiangsu Agricultural Sciences,2022,50(02):205.
[9]李子正,张蕾蕾,褚鹏飞,等.球孢白僵菌植物内生性及其应用特性研究进展[J].江苏农业科学,2022,50(10):1.
 Li Zizheng,et al.Research progress on plant endophytism and its application characteristics of Beauveria bassiana[J].Jiangsu Agricultural Sciences,2022,50(02):1.

备注/Memo

备注/Memo:
收稿日期:2015-11-24
基金项目:国家花生产业技术体系专项(编号:CARS-14)。
作者简介:张丹丹(1990—),女,河北邯郸人,硕士研究生,研究方向为害虫生物防治及分子生物学。E-mail:1404547846@qq.com。
通信作者:李瑞军,博士,副教授,研究方向为害虫生物防治及农业生态安全。E-mail:liruijun99@sina.com。
更新日期/Last Update: 2017-01-20