|本期目录/Table of Contents|

[1]叶维雁,吴海波,刘惠民,等.2株番木瓜根际促生菌的解磷解钾作用[J].江苏农业科学,2017,45(06):247-251.
 Ye Weiyan,et al.Dissolving phosphorus and potassium by two strains of papaya rhizosphere-promoting bacteria[J].Jiangsu Agricultural Sciences,2017,45(06):247-251.
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第45卷
期数:
2017年06期
页码:
247-251
栏目:
资源与环境
出版日期:
2017-04-20

文章信息/Info

Title:
Dissolving phosphorus and potassium by two strains of papaya rhizosphere-promoting bacteria
作者:
叶维雁12 吴海波1 刘惠民1 熊智1 王樱潼1
1.西南林业大学国家林业局西南地区生物多样性保育重点实验室,云南昆明 650224;
2.广西壮族自治区亚热带作物研究所,广西南宁 530001
Author(s):
Ye Weiyanet al
关键词:
番木瓜根际促生菌解磷解钾鉴定肠杆菌层芽孢杆菌属
Keywords:
-
分类号:
S182
DOI:
-
文献标志码:
A
摘要:
用平板涂布、划线与固体平板-透明圈法从番木瓜根际土壤中筛选出2株解磷解钾细菌(PK-1、PK-2)。通过摇瓶培养,测定PK-1、PK-2发酵液中水溶性磷、水溶性钾含量和磷、钾总量;分析PK-1、PK-2解磷解钾能力;对PK-1、PK-2进行分子鉴定。结果表明,PK-1溶解无机磷能力的溶量为1.63 μg/mL,PK-2溶量为 17.26 μg/mL,溶解无机磷能力表现为PK-1﹤PK-2,即PK-2具有更强的解无机磷能力;PK-1溶解有机磷能力的溶量为 8.49 μg/mL,PK-2溶量为2.38 μg/mL,溶解有机磷能力表现为PK-1>PK-2,即PK-1的解有机磷能力更好;PK-1溶解钾能力的溶量为8.15 μg/mL,PK-2溶量为8.49 μg/mL,溶解钾能力表现为PK-1﹤PK-2,PK-1 和PK-2的解钾能力接近。经16S rDNA序列分析,初步鉴定PK-1为肠杆菌属(Enterobacter),PK-2为芽孢杆菌属(Bacillus)。供试菌株PK-1和PK-2具有良好的解磷解钾功能,可为进一步研制专用的番木瓜微生物肥料提供菌种资源和依据。
Abstract:
-

参考文献/References:

[1]康贻军,程洁,梅丽娟,等. 植物根际促生菌的筛选及鉴定[J]. 微生物学报,2010,50(7):853-861.
[2]Ahmad F,Ahmad I,Khan M S. Screening of free-living rhizospheric bacteria for their multiple plant growth promoting activities[J]. Microbiological Research,2008,163(2):173-181.
[3]Verma J P,Yadav J,Tiwari K N,et al. Impact of plant growth promoting rhizobacteria on crop production[J]. International Journal of Agricultural Research,2010,5(11):954-983.
[4]Sheng X F,Huang W Y. The conditions of releasing K-releasing by a silicate bacterial strain NBT[J]. Agric Sci in China,2002,1(6):595-604.
[5]Adesemoye A O,Kloepper J W. Plant-microbes interactions in enhanced fertilizer-use efficiency[J]. Applied Microbiology and Biotechnology,2009,85(1):1-12.
[6]胡江春,薛德林,马成新. 植物根际促生菌(PGPR)的研究与应用前景[J]. 应用生态学报,2004,15(10):963-966.
[7]Almora K,Pino J A,Hernandez M,et al. Evaluation of volatiles from ripening papaya(Carica papaya L.,var. Maradol Roja)[J]. Food Chemistry,2004,86(1):127-130.
[8]Dawkins G,Hewitt H,Wint Y,et al. Antibacterial effects of Carica papaya fruit on common wound organisms[J]. The West Indian Medical Journal,2003,52(4):290-292.
[9]万璐,康丽华,廖宝文,等. 红树林根际解磷菌分离、培养及解磷能力的研究[J]. 林业科学研究,2004,17(1):89-94.
[10]中国土壤学会. 土壤农业化学分析方法[M]. 北京:中国农业科技出版社,1999.
[11]Bochner B R. New technologies to assess genotype-phenotype relationships[J]. Nature Reviews Genetics,2003,4(4):309-314.
[12]Felsenstein J. Confidence limits on phylogenies:an approach using the bootstrap[J]. Evolution,1985,39(4):783-791.
[13]Nautiyal C S. An efficient microbiological growth medium for screening phosphate solubilizing microorganisms[J]. FEMS Microbiology Letters,1999,170(1):265-270.
[14]王舒,张林平,张扬,等. 红壤区油茶根际解磷细菌的筛选、鉴定及其解磷能力[J]. 林业科学研究,2015,28(3):409-416.
[15]罗华元,常寿荣,王绍坤,等. 云烟高端品牌植烟区根际土壤高效解钾菌的筛选[J]. 西南农业学报,2011,24(5):1813-1817.
[16]王岳坤,于飞,唐朝荣. 海南生态区植物根际解磷细菌的筛选及分子鉴定[J]. 微生物学报,2009,49(1):64-71.
[17]任嘉红,刘辉,吴晓蕙,等. 南方红豆杉根际溶无机磷细菌的筛选、鉴定及其促生效果[J]. 微生物学报,2012,53(3):295-303.
[18]刘璇,孔凡玉,张成省,等. 烟草根际解钾菌的筛选与鉴定[J]. 中国烟草科学,2012,33(3):28-31.
[19]秦文旺,牧耀贵,吕利华,等. 一株解钾高活性胶质芽孢杆菌的筛选与育种[J]. 山西农业科学,2015,43(4):434-438.

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备注/Memo

备注/Memo:
收稿日期:2016-06-15
基金项目:云南省森林灾害预警与控制重点实验室开放基金(编号:ZK14A101);云南省教育厅科学研究基金(编号:515006017);西南林业大学林学一级学科博士研究生创新基金。
作者简介:叶维雁(1988—),男,广西北海人,硕士,主要从事果树生物技术研究。E-mail:flyingywy@163.com。
通信作者:吴海波,博士,讲师,主要从事森林培育、经济林栽培方面的教学与研究。E-mail:787337247@qq.com。
更新日期/Last Update: 2017-03-20