|本期目录/Table of Contents|

[1]姚锐,王义强,谭益民,等.激素处理对北美香柏扦插繁殖及相关氧化酶活性的影响[J].江苏农业科学,2017,45(11):97-102.
　Yao Rui,et al.Effects of hormone treatment on cutting propagation and related oxidase activity of Thuja occidentalis[J].Jiangsu Agricultural Sciences,2017,45(11):97-102.
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激素处理对北美香柏扦插繁殖
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第45卷
期数:
2017年11期
页码:
97-102
栏目:
园艺与林学
出版日期:
2017-06-05

文章信息/Info

Title:
Effects of hormone treatment on cutting propagation and related oxidase activity of Thuja occidentalis
作者:
姚锐1;  王义强1; 2;  谭益民2;  黄瑞春3;  米小琴3
1.中南林业科技大学生命科学与技术学院，湖南长沙 410004；
2.中南林业科技大学省部经济林培育与保护重点实验室，湖南长沙 410004； 3.湘西州生态实验站北美香柏试验基地，湖南吉首 416713
Author(s):
Yao Rui; et al
关键词:
北美香柏; 扦插; 生根; 氧化酶活性; 3-吲哚丁酸钾盐(K-IBA); 萘乙酸钾盐(K-NAA)
Keywords:
-
分类号:
S723.1+32
DOI:
-
文献标志码:
A
摘要:
为了研究提高北美香柏Green Giant扦插生根效果的方法及氧化酶活性对其生根的影响，以半木质化北美香柏Green Giant插穗为材料，使用激素ABT生根粉、K-IBA(3-吲哚丁酸钾盐)、K-NAA(萘乙酸钾盐)进行处理，激素质量浓度分别设置为2、6、10 g/L，处理时间分别为5、15、25 s，采用正交试验设计，寻求插穗最优生根组合。使用6 g/L K-IBA处理Green Giant插穗15 s，以清水处理作对照，研究不定根形成过程中过氧化物酶(POD)、多酚氧化酶(PPO)、吲哚乙酸氧化酶(IAAO)活性的动态变化。扦插繁殖正交试验结果表明，6 g/L K-IBA处理插穗5 s，其生根率高达91.67%，平均生根数为17.53条，平均根长达到5.01 cm；正交试验极差分析及多重比较结果表明，6 g/L K-IBA 处理插穗15 s为北美香柏扦插生根最优组合方案。生根过程中氧化酶活性变化分析结果表明，在POD、PPO活性峰值和IAAO活性谷值时期，伴随不定根起始，说明高活性的POD、PPO和低活性的IAAO共同促进了不定根的形成。
Abstract:
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备注/Memo

备注/Memo:
收稿日期：2017-02-27
基金项目：国家林业局“948”项目(编号：2013-4-25)。
作者简介：姚锐(1990—)，女，湖北黄冈人，硕士研究生，研究方向为生物化学与分子生物学。E-mail：1569696194@qq.com。
通信作者：王义强，博士，教授，博士生导师，研究方向为林业生物技术、细胞工程。E-mail：wangyiqiang12@163.com。
更新日期/Last Update: 2017-06-05