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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:

第45卷

期数:

2017年24期

页码:

161-164

栏目:

畜牧兽医与水产蚕桑

出版日期:

2017-12-20

文章信息/Info

Title:

Influences of hydrogen peroxide application on dry weight and lipid content of Chlorella sorokiniana

作者:

王元琮;  何冰

江苏省农业科学院种质资源与生物技术研究所，江苏南京 210014

Author(s):

Wang Yuancong; et al

关键词:

小球藻; 过氧化氢; 干质量; 油脂

Keywords:

-

分类号:

S188

DOI:

-

文献标志码:

A

摘要:

以小球藻(Chlorella sorokiniana)LS-2作为研究材料，用不同浓度过氧化氢(H₂O₂)进行处理。结果表明，所有处理均会抑制LS-2的生长。当H₂O₂浓度较低时，对干质量并没有明显影响，只有当H₂O₂浓度达到 1.5 mmol/L 时，LS-2的最大干质量才会显著降低，1.5、2.0 mmol/L H₂O₂处理的LS-2最大干质量分别下降了 9.6%、17.3%。H₂O₂处理对LS-2的油脂总量影响不显著，但是当H₂O₂浓度达到2.0 mmol/L时，藻细胞的油脂含量显著增加，上升了28.6%。实时荧光定量PCR结果显示，尽管2 mmol/L H₂O₂处理使得Rubisco转录发生上调，但是其下游参与卡尔文循环的基因PKa、ALDO的转录水平均下降了50%，说明卡尔文循环受到抑制。参与油脂合成通路的基因ACCase变化不显著，其下游的ACPa、ACPb的转录水平是对照的2倍，暗示油脂合成途径获得了增强。在糖酵解通路中，ENL上调显著，但其下游的PKa、PKb转录水平的变化不明显，说明糖酵解途径没有受到抑制。由基因表达数据可知，当微藻处于胁迫环境中时，能够将用于生长的能量转化用于合成油脂并存储起来。

Abstract:

-

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备注/Memo

备注/Memo:

收稿日期：2016-06-30
基金项目：国家自然科学基金(编号：31400248)。
作者简介：王元琮(1984—)，男，江苏南京人，博士，助理研究员，主要从事活性小分子的筛选及其提高微藻产油量的机制研究。Tel：(025)84391352；E-mail：wangyuancong@163.com。

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更新日期/Last Update: 2017-12-20