|本期目录/Table of Contents|

[1]姚浩轲,方亚坤,周配,等.微生物法生产甘油酸发酵培养基的优化[J].江苏农业科学,2018,46(03):255-258.
 Yao Haoke,et al.Optimization of fermentation medium for glyceric acid production by microorganism[J].Jiangsu Agricultural Sciences,2018,46(03):255-258.
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微生物法生产甘油酸发酵培养基的优化(PDF)
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第46卷
期数:
2018年03期
页码:
255-258
栏目:
资源与环境
出版日期:
2018-02-05

文章信息/Info

Title:
Optimization of fermentation medium for glyceric acid production by microorganism
作者:
姚浩轲 方亚坤 周配 刘宇鹏 张海燕
河南大学生命科学学院,河南开封 475004
Author(s):
Yao Haokeet al
关键词:
微生物法甘油酸甘油葡糖杆菌响应面分析法培养基优化
Keywords:
-
分类号:
S188+.4
DOI:
-
文献标志码:
A
摘要:
利用新筛选的葡糖杆菌属菌株日本葡萄糖酸杆菌(Gluconobacter japonicus)CGMCC12425氧化甘油生产甘油酸。单因素试验表明,甘油浓度、氮源浓度和金属离子浓度对发酵影响较显著;利用响应面分析法对培养基成分进行优化,建立各因素对甘油酸产量的数学模型;在甘油140.870 g/L、蛋白胨9.590 g/L、MnSO4 0.512 g/L 条件下甘油酸的最大估计值为51.220 g/L,优化后甘油酸产量(51.360 g/L)比优化前(37.620 g/L)提高36.52%,与预测的极值基本符合。
Abstract:
-

参考文献/References:

[1]Habe H,Fukuoka T,Kitamoto D,et al. Biotransformation of glycerol to D-glyceric acid by Acetobacter tropicalis[J]. Applied Microbiology and Biotechnology,2009,81(6):1033-1039.
[2]Habe H,Fukuoka T,Kitamoto D,et al. Biotechnological production of D-glyceric acid and its application[J]. Applied Microbiology and Biotechnology,2009,84(3):445-452.
[3]Habe H,Shimada Y,Yakushi T,et al. Microbial production of glyceric acid,an organic acid that can be mass produced from glycerol[J]. Applied and Environmental Microbiology,2009,75(24):7760-7766.
[4]Sato S,Morita T,Kitamoto D,et al. Change in product selectivity during the production of glyceric acid from glycerol by Gluconobacter strains in the presence of methanol[J]. AMB Express,2013,3(1):20-26.
[5]Habe H,Shimada Y,Fukuoka T,et al. Production of glyceric acid by Gluconobacter sp. NBRC3259 using raw glycerol[J]. Bioscience,Biotechnology,and Biochemistry,2009,73(8):1799-1805.
[6]Habe H,Sato S,Fukuoka T,et al. Effect of glyceric acid calcium salt on the viability of ethanol-dosed gastric cells[J]. Journal of Oleo Science,2011,60(11):585-590.
[7]Sato S,Morita T,Fukuoka T,et al. Microbial resolution of DL-glyceric acid for L-glyceric acid production with newly isolated bacterial strains[J]. Journal of Bioscience and Bioengineering,2015,119(5):554-557.
[8]Habe H,Fukuoka T,Sato S,et al. Synthesis and evaluation of dioleoyl glyceric acids showing antitrypsin activity[J]. Journal of Oleo Science,2011,60(6):327-331.
[9]刘宇鹏,郑璞,孙志浩,等. 采用离子排斥色谱法分析发酵液中的琥珀酸等代谢产物[J]. 食品与发酵工业,2006,32(12):119-123.
[10]方亚坤,靳魁奇,刘宇鹏,等. 离子排斥色谱法分析发酵液中甘油酸等代谢产物[J]. 食品与发酵工业,2015,41(7):171-174.

相似文献/References:

[1]方亚坤,周配,张磊,等.1株高产甘油酸菌株的筛选与鉴定[J].江苏农业科学,2017,45(04):224.
 Fang Yakun,et al.Screening and identification of a strain with high yield of glyceric acid[J].Jiangsu Agricultural Sciences,2017,45(03):224.
[2]周配,张磊,方亚坤,等.静息细胞生物转化甘油生产甘油酸[J].江苏农业科学,2017,45(12):216.
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备注/Memo

备注/Memo:
收稿日期:2016-09-14
基金项目:国家自然科学基金(编号:31400608);河南大学科研项目(编号:xxjc20140009);河南省科技厅攻关项目(编号:152102210255);河南省高校青年骨干教师资助计划(编号:2014GGJS-157);河南省博士后科研基金。
作者简介:姚浩轲(1993—),女,河南巩义人,硕士研究生,主要从事发酵工程研究。E-mail:975312795@qq.com。
通信作者:张海燕,博士,副教授,主要从事生物工程研究。E-mai
更新日期/Last Update: 2018-02-05