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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:

第46卷

期数:

2018年06期

页码:

27-33

栏目:

生物技术

出版日期:

2018-03-20

文章信息/Info

Title:

Molecular cloning and transient gene expression of SrUGT76G1 promoter in Stevia rebaudiana

作者:

杨永恒;  徐晓洋;  孙玉明;  原海燕;  刘清泉;  张永侠;  王银杰;  黄苏珍;  佟海英

江苏省中国科学院植物研究所，江苏南京 210014

Author(s):

Yang Yongheng; et al

关键词:

甜菊; SrUGT76G1启动子; 调控元件; 植物表达载体; 基因瞬时表达

Keywords:

-

分类号:

S566.901

DOI:

-

文献标志码:

A

摘要:

甜菊苷(stevioside，St)和莱鲍迪苷A(rebaudioside A，R-A)是甜菊叶片中两大主要糖苷组分，甜菊糖苷生物合成途径的研究表明SrUGT76G1基因在St向R-A转化中起关键作用。为了进一步研究SrUGT76G1基因的表达调控，本试验采用hiTAIL-PCR的方法，经过2次步移从甜菊基因组中克隆到SrUGT76G1基因翻译起始位点上游 2 283 bp 的启动子序列。采用PlantCARE、PLACE等在线工具对序列进行分析，结果显示在这段序列上共有475个顺式调控元件，除了TATA-box、CAAT-box、MYB结合位点等典型的保守元件外，还包括光照、干旱、厌氧等环境因子响应元件，生长素、赤霉素等植物激素响应元件，芽、胚乳等组织特异性表达元件等。为了初步研究SrUGT76G1基因启动子的功能，本试验通过克隆SrUGT76G1转录起始位点上游1 989 bp带酶切位点的序列，将其替换植物表达载体pCAMBIA1301-220中的CaMV35S启动子，连接下游的GUS报告基因，构建重组植物表达载体pCAMBIA1301-220-SrUGT76G1P，以pCAMBIA1301-220载体作对照，通过农杆菌(EHA105)真空渗透法转入拟南芥和甜菊幼苗。瞬时表达结果表明，该SrUGT76G1启动子序列能驱动GUS基因在拟南芥和甜菊叶片中表达，具有启动子活性。

Abstract:

-

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相似文献/References:

[1]杨永恒,黄苏珍.NaCl胁迫下甜菊不同耐盐性单株的生长及生理响应[J].江苏农业科学,2013,41(08):87.
　Yang Yongheng,et al.Growth and physiological response of Stevia rebaudiana Bertoni plants with different salt tolerance under salt stress[J].Jiangsu Agricultural Sciences,2013,41(06):87.
[2]杨永恒,侯孟兰,张婷,等.甜菊β-葡萄糖苷酶活性与甜菊糖苷含量变化的研究[J].江苏农业科学,2020,48(11):187.
　Yang Yongheng,et al.Study on changes of beta-glucosidase activity and steviol glycosides content in Stevia rebaudiana[J].Jiangsu Agricultural Sciences,2020,48(06):187.

备注/Memo

备注/Memo:

收稿日期：2017-10-29
基金项目：国家自然科学基金青年科学基金(编号：31601371)；江苏省自然科学基金青年基金(编号：BK20160600)。
作者简介：杨永恒(1985—)，女，陕西洋县人，博士，助理研究员，主要从事甜菊育种方面的研究。E-mail：yyh8576@126.com。
通信作者：佟海英，高级实验师，主要从事甜菊栽培繁育方面的研究。E-mail：njtonghy@163.com。

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更新日期/Last Update: 2018-03-20