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[1]韩林贺,丁安明,孔英珍.普通烟草PMEI家族的鉴定与表达分析[J].江苏农业科学,2018,46(09):34-43.
　Han Linhe,et al.Genome-wide identification and expression analysis of the PMEI family in Nicotiana tabacum[J].Jiangsu Agricultural Sciences,2018,46(09):34-43.
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普通烟草PMEI家族的鉴定与表达分析(PDF)
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第46卷
期数:
2018年09期
页码:
34-43
栏目:
生物技术
出版日期:
2018-05-05

文章信息/Info

Title:
Genome-wide identification and expression analysis of the PMEI family in Nicotiana tabacum
作者:
韩林贺1; 2;  丁安明1;  孔英珍1
1.中国农业科学院烟草研究所/中国农业科学院烟草遗传改良与生物技术重点开放实验室，山东青岛 266101；
2.中国农业科学院研究生院，北京 100081
Author(s):
Han Linhe; et al
关键词:
PMEI; 基因家族; 生物信息; 荧光定量PCR; 普通烟草
Keywords:
-
分类号:
S572.032
DOI:
-
文献标志码:
A
摘要:
果胶是细胞壁的重要组成部分，它不仅在细胞之间的连接上起着关键作用，还能维持细胞壁结构稳定和抵御病原的侵害。细胞壁中的果胶甲酯酶抑制剂(pectin methyl ester enzyme inhibitor，简称PMEI)可以调控果胶甲酯酶(pectin methyl ester enzyme，简称PME)对果胶的去甲酯化作用，从而引起细胞壁结构和功能特性的改变。为深入研究PMEI基因家族在烟草生长发育过程中的功能，采用生物信息学方法，在普通烟草基因组中共鉴定出90个PMEI基因家族成员，并对其进行基因结构、系统进化树、蛋白理化性质、保守结构域、表达分析和基因本体(gene ontology，简称GO)分析。结果表明，烟草PMEI基因家族可以分为5个亚家族，在进化树中形成明显不同的分支，同一亚家族中各成员的基因结构、编码蛋白的理化性质及保守域呈现较高的一致性。每个成员都含有由4个反向平行α螺旋组成的PMEI结构域，结构域序列中有4个极为保守的半胱氨酸残基，形成2个二硫键。GO分析结果显示，所有烟草的PMEI蛋白都具有酶抑制活性，大部分成员参与细胞壁的修饰过程。PMEI成员在烟草不同组织中均有表达，其中在茎、叶脉、幼苗叶和幼苗根中的基因表达数较多；第Ⅳ、Ⅴ亚家族成员在幼根、幼叶中表达量较高。研究结果将为烟草PMEI家族的深入研究提供依据，为进一步解析PMEI基因的功能奠定基础。
Abstract:
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备注/Memo

备注/Memo:
收稿日期：2017-02-06
基金项目：国家科技支撑计划(编号：2015BAD15B03-05)；国家自然科学基金(编号：31470291、31670302)。
作者简介：韩林贺(1993—)，女，山东邹城人，硕士研究生，研究方向为作物遗传育种。E-mail：nirvanah@126.com。
通信作者：孔英珍，博士，研究员，博士生导师，研究方向为细胞壁多糖合成。E-mail：kongyingzhen@163.com。
更新日期/Last Update: 2018-05-05