|本期目录/Table of Contents|

[1]熊军波,刘洋,田宏,等.紫花苜蓿幼苗茎叶响应盐胁迫的双向电泳比较分析[J].江苏农业科学,2018,46(14):148-153.
　Xiong Junbo,et al.Two dimensional electrophoresis analysis of salt-stress response proteins in leaf and stem of alfalfa seedlings[J].Jiangsu Agricultural Sciences,2018,46(14):148-153.
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紫花苜蓿幼苗茎叶响应盐胁迫的双向电泳比较分析(PDF)
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第46卷
期数:
2018年第14期
页码:
148-153
栏目:
畜牧兽医与水产蚕桑
出版日期:
2018-07-25

文章信息/Info

Title:
Two dimensional electrophoresis analysis of salt-stress response proteins in leaf and stem of alfalfa seedlings
作者:
熊军波;  刘洋;  田宏;  张鹤山;  陈明新
湖北省农业科学院畜牧兽医研究所/动物胚胎工程及分子育种湖北省重点实验室，湖北武汉 430064
Author(s):
Xiong Junbo; et al
关键词:
紫花苜蓿; 盐胁迫; 蛋白质; 耐盐调节机制; 双向电泳; 光合作用; 胁迫防御应答; 碳水化合物代谢; 转录调控; 信号传导; 细胞分化; 次生代谢
Keywords:
-
分类号:
S541+.101
DOI:
-
文献标志码:
A
摘要:
紫花苜蓿是全球栽培最为广泛的豆科牧草，土壤盐碱化是导致苜蓿减产的主要因素之一。从蛋白质组学角度对紫花苜蓿苗期响应盐胁迫的地上部蛋白质组进行研究，以揭示紫花苜蓿耐盐调节机制，发掘耐盐应答相关蛋白。6日龄紫花苜蓿中苜一号幼苗在0、200 mmol/L NaCl处理9 d后，采用双向电泳分别分离了其茎叶部蛋白组，在对照和处理间20个蛋白质点丰度发生2倍以上变化，采用MALDI-TOF-TOF/MS对差异蛋白进行鉴定，最终有14个蛋白鉴定成功。功能分析表明，这些蛋白主要参与光合作用、胁迫防御应答、碳水化合物代谢、转录调控和信号传导、细胞分化和次生代谢。
Abstract:
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备注/Memo

备注/Memo:
收稿日期：2016-05-17
基金项目：国家自然科学基金(编号：31402130)；湖北省重点实验室开放课题(编号：2012ZD201)；湖北农业科学院青年科学基金(编号：2012NKYJJ12)。
作者简介：熊军波(1982—)，男，湖北天门人，博士，副研究员，主要从事牧草分子育种研究。Tel：(027)87380139；E-mail：jbx9715@126.com。
通信作者：刘洋，研究员，主要从事牧草遗传育种研究。E-mail：liuyang430209@126.com。
更新日期/Last Update: 2018-07-20