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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:

第41卷

期数:

2013年08期

页码:

18-22

栏目:

生物技术

出版日期:

2013-08-25

文章信息/Info

Title:

Screening and analysis of TabZIP60 transcription factor induced by salt stress

作者:

刘骥¹;  王燕¹;  郭建华¹;  刘晓兰¹;  孔秀英²

1.齐齐哈尔大学食品与生物工程学院，黑龙江齐齐哈尔 161006； 2.中国农业科学院作物研究所，北京 100081

Author(s):

Liu Ji; et al

关键词:

TabZIP60; 盐胁迫; 转录因子; 序列分析

Keywords:

-

分类号:

Q785

DOI:

-

文献标志码:

A

摘要:

筛选与鉴定耐盐相关基因是培育抗盐品种的基础。植物转录因子在植物的生长发育及其对外界环境的反应中起着重要作用。利用小麦转录因子全长cDNA文库，运用Re-Northern和半定量RT-PCR相结合的方法，从小麦全长转录因子cDNA文库的526个转录因子进行了盐胁迫筛选，获得了与小麦抗盐相关的转录因子TabZIP60，分析这个转录因子的序列并进行了功能分析，获得如下结果：TabZIP60 的长度是 1 741 bp，编码338个氨基酸；TabZIP60受高盐诱导后表达量明显升高；GFP亚细胞定位表明该转录因子是一个核定位蛋白。

Abstract:

-

参考文献/References:

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备注/Memo

备注/Memo:

收稿日期：2013-04-07
基金项目：引进国际先进农业科学技术计划(“948”计划)(编号：2005-Z47)。
作者简介：刘骥(1983—)，男，黑龙江齐齐哈尔人，硕士，助教，主要从事植物分子生物学方面研究。E-mail：liuji83@163.com。
通信作者：孔秀英，博士，研究员，研究方向为植物分子生物学。E-mail：xykong@mail.caas.net.cn。

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更新日期/Last Update: 2013-08-25