|本期目录/Table of Contents|

[1]岳丹,王磊,乔莉娟,等.高效纤维素降解菌株筛选及其复合微生物菌剂在有机堆肥中的应用效果[J].江苏农业科学,2018,46(17):273-276.
 Yue Dan,et al.Screening of high-efficiency cellulose degradation strains and application effect of its compound microbial agents in organic compost[J].Jiangsu Agricultural Sciences,2018,46(17):273-276.
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高效纤维素降解菌株筛选及其复合微生物菌剂
在有机堆肥中的应用效果
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第46卷
期数:
2018年第17期
页码:
273-276
栏目:
资源与环境
出版日期:
2018-09-05

文章信息/Info

Title:
Screening of high-efficiency cellulose degradation strains and application effect of its compound microbial agents in organic compost
作者:
岳丹1 王磊2 乔莉娟2 李丹花2 昝立峰2
1.邯郸学院实验与实训中心,河北邯郸 056005; 2.邯郸学院生命科学与工程学院,河北邯郸 056005
Author(s):
Yue Danet al
关键词:
纤维素高效降解菌筛选鉴定复合菌剂堆肥
Keywords:
-
分类号:
X71;S182
DOI:
-
文献标志码:
A
摘要:
采用羧甲基纤维素钠(CMC-Na)平板法和刚果红染色法从竹林表层土壤中分离出高效纤维素降解菌株,经16S rDNA序列同源性比对鉴定菌株后,通过液体发酵法将菌株制备成复合菌剂,将复合菌剂添加至堆肥物料中进行效果验证。结果表明,所得菌株L1和L2为小麦苍白杆菌(Ochrobactrum tritici),L-3为中华根瘤菌属(Sinorhizobium sp ).,L-4为绿针假单胞菌(Pseudomonas chlororaphis),L5为枯草芽胞杆菌(Bacillus subtilis)。将制备的微生物复合菌剂添加到堆肥中进行堆肥试验,结果表明,接种复合微生物菌剂的菌剂组堆体最高温度为62.2 ℃,高温维持10 d,对照组堆体最高温度为58 ℃,高温维持7 d;菌剂组碳氮比从30下降到15.4,对照组碳氮比从30.6下降到18;菌剂组堆肥纤维素含量降至24.7%,对照组堆肥纤维素含量降至17.3%。菌剂组和对照组堆肥均达到了腐熟无害化标准,接种复合菌剂可以显著提高堆肥温度,缩短堆肥周期,保证堆肥质量。
Abstract:
-

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:2017-12-17
基金项目:河北省高等学校科学技术研究(编号:Z2017058);河北省邯郸市科技计划(编号:1622201002ZC、1423109105、1464601095-5);邯郸学院校内委托项目(编号:14301)。
作者简介:岳丹(1980—),男,河北邯郸人,硕士,讲师,研究方向为微生物菌剂应用。E-mail:yuedan1980@gmail.com。
通信作者:昝立峰,博士,副教授,研究方向为应用微生物。E-mail:tengfei007zlf@126.com。
更新日期/Last Update: 2018-09-05