|本期目录/Table of Contents|

[1]李小义,张效平,赵凤,等.鲟鱼肠道微生物多样性的研究[J].江苏农业科学,2018,46(24):164-167.
 Li Xiaoyi,et al.Study on diversity of gut microbiota of sturgeon[J].Jiangsu Agricultural Sciences,2018,46(24):164-167.
点击复制

鲟鱼肠道微生物多样性的研究(PDF)
分享到:

《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第46卷
期数:
2018年第24期
页码:
164-167
栏目:
畜牧兽医与水产蚕桑
出版日期:
2018-12-20

文章信息/Info

Title:
Study on diversity of gut microbiota of sturgeon
作者:
李小义 张效平 赵凤 孔杰 赵飞 周洲 王艳艳
贵州省农业科学院水产研究所,贵州贵阳 550025
Author(s):
Li Xiaoyiet al
关键词:
肠道微生物鲟鱼高通量多样性
Keywords:
-
分类号:
S917.1
DOI:
-
文献标志码:
A
摘要:
从贵州省水产研究所惠水试验基地采集杂交鲟鱼、养殖水体及饲料等样品,对鲟鱼前肠、中肠及后肠肠道微生物、养殖水体及饲料等样品微生物DNA进行提取扩增,结合宏基因组学测序技术和生物信息学分析等手段,对鲟鱼肠道微生物多样性进行分析。结果表明,在门水平上,变形菌门(Proteobacteria)在前肠、中肠、后肠及水样等样品为主要优势菌群,丰度分别为75.0%、57.4%、66.0%、50.8%。饲料样品中蓝细菌(Cyanobacteria)丰度最高,为 59.5%。属水平分析,前肠样品组G6中,假单胞菌属(Pseudomonas)丰度最高,为23.18%,其次为气单胞菌属(Aeromonas,723%)、不动杆菌属(Acinetobacter,3.73%)、黄杆菌属(Flavobacterium,2.47%)、unidentified Marinilabiaceae(2.43%)、Sedimenticola(2.20%)。中肠样品组G7中,以unidentified Marinilabiaceae(4.22%)、Sedimenticola(3.13%)、地杆菌属(Geobacter,2.60%)、假单胞菌属(Pseudomonas,2.37%)、不动杆菌属(Acinetobacter,1.74%)、气单胞菌属(Aeromonas,1.40%)为主。后肠样品组G8中,主要包括气单胞菌属(Aeromonas,0.52%)、假单胞菌属(Pseudomonas,0.47%)、不动杆菌属(Acinetobacter,0.13%)、Sedimenticola(0.07%)、unidentified Marinilabiaceae(0.06%)、unidentified Chloroplast(0.03%)。前肠、中肠肠道微生物类型及丰度差异不大,后肠细菌和前、中肠内细菌差异明显。
Abstract:
-

参考文献/References:

[1]Flint H J,Scott K P,Louis P,et al. The role of the gut microbiota in nutrition and health[J]. Nature Reviews Gastroenterology & Hepatology,2012,9(10):577-589.
[2]Navarrete P,Espejo R T,Romero J. Molecular analysis of microbiota along the digestive tract of juvenile Atlantic salmon(Salmo salar L.)[J]. Microbial Ecology,2009,57(3):550-561.
[3]Nagalingam N A,Lynch S V. Role of the microbiota in inflammatory bowel diseases[J]. Inflammatory Bowel Diseases,2012,18(5):968-980.
[4]Xiong J B,Zhu J Y,Dai W F,et al. Integrating gut microbiota immaturity and disease-discriminatory taxa to diagnose the initiation and severity of shrimp disease[J]. Microbial Ecology,2017,19(4):1490-1501.
[5]Kan H F,Zhao F Z,Zhang X X,et al. Correlations of gut microbial community shift with hepatic damage and growth inhibition of Carassius auratus induced by pentachlorophenol exposure[J]. Environmental Science & Technology,2015,49(19):11894-11902.
[6]Rolig A S,Mittge E K,Ganz J,et al. The enteric nervous system promotes intestinal health by constraining microbiota composition[J]. PLOS Biology,2017,15(2):e2000689.
[7]钟蕾,向建国,曾丹,等. 饵料对鳡肠道微生物多样性的影响[J]. 水生生物学报,2016,40(4):830-835.
[8]唐杨,刘文亮,宋晓玲,等. 饲料中补充蜡样芽孢杆菌对凡纳滨对虾生长及其肠道微生物组成的影响[J]. 水产学报,2017,41(5):765-774.
[9]Ley R E,Hamady M,Lozupone C,et al. Evolution of mammals and their gut microbes[J]. Science,2008,320(5883):1647-1651.
[10]Methe B A,Nelson K E,Pop M,et al. A framework for human microbiome research[J]. Nature,2012,486(742):215-221.
[11]Roeselers G,Mittge E K,Stephens W Z,et al. Evidence for a core gut microbiota in the zebrafish[J]. The ISME Journal,2011,5(10):1595-1608.
[12]Kim D H,Brunt J,Austin B. Microbial diversity of intestinal contents and mucus in rainbow trout (Oncorhynchus mykiss)[J]. Journal of Applied Microbiology,2007,102(6):1654-1664.
[13]Wong A C,Chaston J M,Douglas A E. The inconstant gut microbiota of Drosophila species revealed by 16S rRNA gene analysis[J]. The ISME Journal,2013,7(10):1922-1932.
[14]Hammer T J,Mcmillan W O,Fierer N. Metamorphosis of a butterfly-associated bacterial community[J]. PLoS One,2014,9(1):e86995-e87002.
[15]Ni J,Yan Q,Yu Y,et al. Factors influencing the grass carp gut microbiome and its effect on metabolism[J]. FEMS Microbiology Ecology,2014,87(3):704-714.
[16]Tzeng T D,Pao Y Y,Chen P C,et al. Effects of host phylogeny and habitats on gut microbiomes of oriental river prawn (Macrobrachium nipponense)[J]. PLoS One,2015,10(7):e0132860.
[17]李建柱,侯杰,张鹏飞,等. 鱼菜共生模式中不同鱼类肠道微生物群落结构的比较[J]. 南方水产科学,2016,12(6):42-50.
[18]兰阿峰,杨曼,郭素芬,等. 免培养法对大鲵肠道微生物多样性的研究[J]. 微生物学通报,2014,41(7):1342-1349.
[19]Ghorbani-Choboghlo H,Khosravi A R,Sharifzadeh A,et al. Gastrointestinal microflora of captured stellate sturgeon (Acipenser stellatus,Pallas,1771) from Southeast Caspian Sea,Iran[J]. Iranian Journal of Fisheries Sciences,2014,13(2):319-329.
[20]Geraylou Z,Vanhove M P,Souffreau C A,et al. In vitro selection and characterization of putative probiotics isolated from the gut of Acipenser baerii (Brandt,1869)[J]. Aquaculture Research,2014,45(2):341-352.
[21]王纯,倪加加,颜庆云,等. 草鱼与团头鲂肠道菌群结构比较分析[J]. 水生生物学报,2014,38(5):868-875.
[22]Fujimura K E,Slusher N A,Cabana M D. Role of the gut microbiota in defining human health[J]. Expert Review of Anti-Infective Therapy,2010,8(4):435-454.
[23]Moran D,Turner S J,Clements K D. Ontogenetic development of the gastrointestinal microbiota in the marine herbivorous fish Kyphosus sydneyanus[J]. Microbial Ecology,2005,49(4):590-597.

相似文献/References:

[1]孙勇,曹小迎,蒋继宏,等.华北大黑鳃金龟幼虫肠道菌的分离及对农药的降解[J].江苏农业科学,2016,44(02):151.
 Sun Yong,et al.Isolation and pesticides degradation of intestinal microflora from Holotrichia oblita larvae[J].Jiangsu Agricultural Sciences,2016,44(24):151.
[2]冯飞斐,王一丁.应用Illumina高通量测序技术分析中华真地鳖肠道微生物多样性[J].江苏农业科学,2018,46(04):167.
 Feng Feifei,et al.Study on diversity of intestinal microbiota in Eupolyphage sinensis Walker using Illumina high-throughput sequencing system[J].Jiangsu Agricultural Sciences,2018,46(24):167.
[3]刘均,刘雅丽,夏兵.茶多酚对动物健康的保护作用研究进展[J].江苏农业科学,2018,46(12):14.
 Liu Jun,et al.Research progress of protective effect of tea polyphenols on animal health[J].Jiangsu Agricultural Sciences,2018,46(24):14.
[4]韩晓云,刘鹏,王震,等.核桃青皮提取液在腹泻小鼠体内的抑菌效果[J].江苏农业科学,2019,47(24):162.
 Han Xiaoyun,et al.Antimicrobial activity of extracts from walnut peel to mice in vivo[J].Jiangsu Agricultural Sciences,2019,47(24):162.
[5]黄薇,刘兰英,罗土炎,等.鳗鲡肠道微生物抗性基因组成特征的初步分析[J].江苏农业科学,2020,48(21):57.
 Huang Wei,et al.Preliminary analysis of antibiotic resistance genes in eel intestinal microbiota[J].Jiangsu Agricultural Sciences,2020,48(24):57.
[6]耿宁宁,戴竹青,牛丽影,等.膳食纤维调节肠道微生物对机体健康的影响研究进展[J].江苏农业科学,2021,49(7):51.
 Geng Ningning,et al.Effects of dietary fiber regulating human intestinal microflora on body health:a review[J].Jiangsu Agricultural Sciences,2021,49(24):51.
[7]杨晓志,顾文婕,吉俊玲,等.五味子对产蛋期藏鸡肠道微生物菌群的影响[J].江苏农业科学,2023,51(4):180.
 Yang Xiaozhi,et al.Impacts of Schisandra chinensis on intestinal microbiota in Tibetan chicken during egg-laying period[J].Jiangsu Agricultural Sciences,2023,51(24):180.
[8]岑明珠,张宇煊,郑朝军,等.肠道微生物对鸡免疫功能的影响综述[J].江苏农业科学,2023,51(11):10.
 Cen Mingzhu,et al.Influence of gut microbes on immune function of chickens: a review[J].Jiangsu Agricultural Sciences,2023,51(24):10.
[9]易春燕,刘旭,王燕平,等.草地贪夜蛾与黏虫肠道微生物的多样性分析[J].江苏农业科学,2024,52(5):140.
 Yi Chunyan,et al.Analysis of gut microbiota diversity of Spodoptera frugiperda and Mythimna separate[J].Jiangsu Agricultural Sciences,2024,52(24):140.

备注/Memo

备注/Memo:
收稿日期:2017-07-28
基金项目:贵州省科学技术基金(编号:黔科合J字LKN[2013]11号);贵州省农业科学院项目(编号:黔农科院院专项[2014]012号);国家自然科学基金(编号:31660746);贵州省特色水产产业技术体系项目(编号:GZCYTX2013)。
作者简介:李小义(1988—),女,贵州贵阳人,硕士,助理研究员,从事水产养殖研究。E-mail:lxyw2012@yeah.net。
通信作者:王艳艳,硕士,助理研究员,从事水产养殖研究。E-mai
更新日期/Last Update: 2018-12-20