|本期目录/Table of Contents|

[1]周晓伦,万建新,王卫卫,等.一株提高植物幼苗耐受Cr6+细菌(Exiguobacterium sp.S2)的分离与鉴定[J].江苏农业科学,2019,47(07):273-277.
　Zhou Xiaolun,et al.Isolation and identification of Exigubacterium sp. strain S2 improving the tolerance of plant seedlings to heavy metal Cr(Ⅵ)[J].Jiangsu Agricultural Sciences,2019,47(07):273-277.
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一株提高植物幼苗耐受Cr6+细菌
(Exiguobacterium sp.S2)的分离与鉴定(PDF)
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第47卷
期数:
2019年第07期
页码:
273-277
栏目:
资源与环境
出版日期:
2019-05-10

文章信息/Info

Title:
Isolation and identification of Exigubacterium sp. strain S2 improving the tolerance of plant seedlings to heavy metal Cr(Ⅵ)
作者:
周晓伦1;  万建新1;  王卫卫2;  张伟1;  姚彦红1;  高芸1
1.甘肃医学院病原生物学教研室，甘肃平凉 744000； 2.西北大学西部资源生物与现代生物技术教育部重点实验室，陕西西安 710069
Author(s):
Zhou Xiaolun; et al
关键词:
玉米幼苗; Cr6+耐受菌株; ACC脱氨酶; 吲哚乙酸; 微小杆菌
Keywords:
-
分类号:
X53；S182
DOI:
-
文献标志码:
A
摘要:
植物促生菌因其具有对植物生长促进及增强抗逆性等优点，在植物-微生物联合修复重金属污染土壤中具有良好的应用潜力，能为环境生物修复以及工农业生产提供优良菌种资源，实现其更大范围的应用。以甘肃省平凉市污染土壤中分离得到的Cr6+耐受菌株为目的菌，测定菌株的促植物生长特性(产IAA、溶磷、ACC脱氨酶活性)，采用改良的Belimov方法筛选出1株促生特性较好的菌株，进行生理生化及16S rRNA基因序列分析鉴定。初步分离得到32株Cr6+耐受菌株，根据改良的Belimov方法筛选出1株S2菌株，通过生理生化及16S rRNA鉴定S2菌株为Exiguobacterium sp.，GenBank登录号为MH180821。玉米幼苗生长试验表明，与不同Cr6+浓度处理组相比，接种了S2菌株的玉米幼苗根长、茎长、叶面积都有显著提高，平均根长分别增加95.74%、41.34%、194.12%，平均茎长分别增加32.03%、-30.13%、28.96%，平均叶面积分别增加73.94%、35.17%、26.92%，平均鲜质量分别增加33.33%、3362%、-20.00%，其显著提高了玉米幼苗对Cr6+的耐受性。该研究表明，Exiguobacterium sp.S2在污染土壤中能够更好地定殖并保护促植物生长能力的发挥，为重金属污染土壤的植物-微生物联合原位修复提供了良好的微生物资源。
Abstract:
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备注/Memo

备注/Memo:
收稿日期：2019-01-21
基金项目：甘肃省高等学校科学研究项目(编号：2017A-146)。
作者简介：周晓伦(1987—)，男，甘肃靖远人，硕士，讲师，主要从事土壤微生物的研究。E-mail：610253095@qq.com。
更新日期/Last Update: 2019-04-05