|本期目录/Table of Contents|

[1]曹坤,管明,陈康,等.一株拮抗禾谷镰刀菌和降解呕吐毒素解淀粉芽孢杆菌的筛选及在饲料贮存中的应用[J].江苏农业科学,2019,47(08):179-183,190.
 Cao Kun,et al.Screening of probiotic Bacillus amyloliquefaciens CPLK1314 with function of antagonizing Fusarium graminearum and degrading vomiting toxin and its application in forage storing[J].Jiangsu Agricultural Sciences,2019,47(08):179-183,190.
点击复制

一株拮抗禾谷镰刀菌和降解呕吐毒素解淀粉
芽孢杆菌的筛选及在饲料贮存中的应用
(PDF)
分享到:

《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第47卷
期数:
2019年第08期
页码:
179-183,190
栏目:
畜牧兽医与水产蚕桑
出版日期:
2019-05-19

文章信息/Info

Title:
Screening of probiotic Bacillus amyloliquefaciens CPLK1314 with function of antagonizing Fusarium graminearum and degrading vomiting toxin and its application in forage storing
作者:
曹坤 管明 陈康 胡彤 林赟 罗楚平
淮阴工学院生命科学与食品工程学院,江苏淮安 223001
Author(s):
Cao Kunet al
关键词:
禾谷镰刀菌呕吐毒素解淀粉芽孢杆菌生物防治赤霉病
Keywords:
-
分类号:
S816.79;S182
DOI:
-
文献标志码:
A
摘要:
禾谷镰刀菌是引起农作物赤霉病的主要病原菌之一,其次生代谢产物呕吐毒素对人和动物有极大的安全危害,呕吐毒素一旦污染饲料不仅会造成巨额的经济损失,更有可能进入食物链威胁到人与动物的健康安全。目前我国鲜有高效的脱毒方法来应对呕吐毒素对于饲料的安全威胁。为解决上述问题,从江苏省淮安市土壤中筛选出1株同时具有禾谷镰刀菌抗性与呕吐毒素降解能力的解淀粉芽孢杆菌。解淀粉芽孢杆菌本身就是优异的饲料益生菌种,用高效液相色谱和质谱联用的方法分析该菌株后发现其含有杆菌霉素L和泛革素2种脂肽类抗生素,对禾谷镰刀菌的抑菌率达60%;经液相色谱检测后表明该菌在14 d后呕吐毒素转化率达到92.440%。数据表明该菌在饲料防霉与脱毒方面表现出良好的应用前景。
Abstract:
-

参考文献/References:

[1]方兴洲. 小麦赤霉病研究进展[J]. 现代农业科技,2014(23):134-135.
[2]Cui L,Selvaraj J N,Xing F G,et al. A minor survey of deoxynivalenol in Fusarium infected wheat from Yangtze-Huaihe river basin region in China[J]. Food Control,2013,30(2):469-473.
[3]Pestka J J. Deoxynivalenol:mechanisms of action,human exposure,and toxicological relevance[J]. Archives of Toxicology,2010,84(9):663-679.
[4]Maresca M,Mahfoud R,Garmy N,et al. The mycotoxin deoxynivalenol affects nutrient absorption in human intestinal epithelial cells[J]. The Journal of Nutrition,2002,132(9):2723-2731.
[5]Koch P. State of the art of trichothecenes analysis[J]. Toxicology Letters,2004,153(1):109-112.
[6]Morooka N,Uratsuji N,Yoshizawa T,et al. Studies on the toxic substances in barley in fected with Fusarium spp.[J]. Journal of the Food Hygienic Society of Japan,1972,13(5):368-375.
[7]Yoshizawa T,Morooka N. Biological modification of trichothecene mycotoxins:acetylation and deacetylation of deoxynivalenols by Fusarium spp.[J]. Applied Microbiology,1975,29(1):54-58.
[8]Moss M O,Thrane U. Fusarium taxonomy with relation to trichothecene formation[J]. Toxicology Letters,2004,153(1):23-28.
[9]Thuvander A,Wikman C,Gadhasson I. In vitro exposure of human lymphocytes to trichothecenes:individual variation in sensitivity and effects of combined exposure on lymphocyte function[J]. Food and Chemical Toxicology,1999,37(6):639-648.
[10]吴永宁. 现代食品安全科学[M]. 北京:化学工业出版社,2003:311.
[11]Moe N K T,Su M T,Suzuki K,et al. Production of an antibacterial substance by Bacillus mojavensis strain F412 isolated from a Myanmar shrimp product fermented with boiled rice[J]. Fisheries Science,2015,81(4):795-802.
[12]陈哲,黄静,赵佳,等. 解淀粉芽孢杆菌抑菌机制的研究进展[J]. 生物技术通报,2015,31(6):37-41.
[13]Skadsen R W,Hohn T M. Use of Fusarium graminearum transformed with gfp to follow infection patterns in barley and Arabidopsis[J]. Physiological and Molecular Plant Pathology,2004,64(1):45-53.
[14]Priest F G,Goodfellow M,Shute L A,et al. Bacillus amyloliquefaciens sp. nov.,norn. rev.[J]. International Journal of Systematic Bacteriology,1987,37(1):69-71.
[15]Xu Z H,Shao J H,Li B,et al. Contribution of bacillmoycin D in Bacillus amyloliquefaciens SQR9 to antifungal activity and biofilm formation[J]. Applied and Environmental Microbiology,2013,79(3):808-815.
[16]Sutyak K E,Wirawan R E,Aroutcheva A A,et al. Isolation of the Bacillus subtilis antimicrobial peptide subtilosin from the dairy product-derived Bacillus amyloliquefaciens[J]. Journal of Applied Microbiology,2008,104(4):1067-1074.
[17]计坚. 解淀粉芽孢杆菌SC06介导猪肠道免疫机理的研究[D]. 杭州:浙江大学,2011.
[18]沈勇涛. 芽孢杆菌对母猪仔猪微生态的影响及其生长条件优化[D]. 武汉:华中农业大学,2011.
[19]Chen Y,Li J,Xiao P,et al. Isolation and characterization of Bacillus spp. M001 for potential application in turbot(Scophthalmus maximus L.)against Vibrio anguillarum[J]. Aquaculture Nutrition,2016,22(2):374-381.
[20]权春善,王军华,徐洪涛,等. 一株抗真菌解淀粉芽孢杆菌的分离鉴定及其发酵条件的初步研究[J]. 微生物学报,2006,46(1):7-12.
[21]东秀珠,蔡妙英. 常见细菌系统鉴定手册[M]. 北京:科学出版社,2001.

相似文献/References:

[1]孙晓梅,黄金光.禾谷镰刀菌甾醇14α脱甲基酶基因cDNA克隆及生物信息学分析[J].江苏农业科学,2016,44(03):31.
 Sun Xiaomei,et al.cDNA cloning and bioinformatics analysis of sterol 14α-demethylase gene in Fusarium graminearum[J].Jiangsu Agricultural Sciences,2016,44(08):31.
[2]张鹏,邓渊钰,杨学明,等.小麦茎基腐病菌鉴定及不同药剂防治效果分析[J].江苏农业科学,2016,44(11):142.
 Zhang Peng,et al.Identification of wheat stem rot pathogen and analysis of control effects of different pesticides[J].Jiangsu Agricultural Sciences,2016,44(08):142.
[3]侯瑞,金巧军.禾谷镰刀菌真菌毒素DON生物合成途径及调控机制研究进展[J].江苏农业科学,2018,46(17):9.
 Hou Rui,et al.Research progress of biosynthesis approach and regulatory mechanisms of Fusarium graminearum mycotoxin DON[J].Jiangsu Agricultural Sciences,2018,46(08):9.
[4]张悦,施维,李丹,等.禾谷镰刀菌全基因组候选效应因子预测与分析[J].江苏农业科学,2019,47(06):81.
 Zhang Yue,et al.Analysis of candidate effectors from genome of Fusarium graminearum[J].Jiangsu Agricultural Sciences,2019,47(08):81.
[5]戴宝玲,杨华,戴贤君,等.小麦真菌结构分析及其与呕吐毒素含量的关系[J].江苏农业科学,2019,47(12):228.
 Dai Baoling,et al.Analysis of wheat fungi structure and its relationship with vomiting toxins content[J].Jiangsu Agricultural Sciences,2019,47(08):228.
[6]刘双双,孙凯,蔡国栋,等.呕吐毒素对小鼠T淋巴细胞体外活化及相应活化标志分子的影响[J].江苏农业科学,2020,48(04):150.
 Liu Shuangshuang,et al.Effects of deoxynivalenol on activation and relative activation markers of mice T lymphocytes in vitro[J].Jiangsu Agricultural Sciences,2020,48(08):150.
[7]张强,张艳茹,霍云凤,等.禾谷镰刀菌拮抗菌ZQT-31的分离与鉴定[J].江苏农业科学,2021,49(9):80.
 Zhang Qiang,et al.Isolation and identification of antagonistic bacteria ZQT-31 against Fusarium graminearum[J].Jiangsu Agricultural Sciences,2021,49(08):80.
[8]张艳茹,霍云凤,石红利,等.禾谷镰刀菌拮抗菌ZQT-9的鉴定与抑菌活性[J].江苏农业科学,2021,49(18):111.
 Zhang Yanru,et al.Identification and antifungal activity of antagonistic bacteria ZQT-9 against Fusarium graminearum[J].Jiangsu Agricultural Sciences,2021,49(08):111.
[9]王子洋,熊雨洁,冯发运,等.寡雄腐霉对禾谷镰刀菌防效及其产孢诱导剂筛选[J].江苏农业科学,2023,51(18):101.
 Wang Ziyang,et al.Control effect of Pythium oligandrum against Fusarium graminearum and screening of its pathogen inducers[J].Jiangsu Agricultural Sciences,2023,51(08):101.
[10]张强,张艳茹,霍云凤,等.禾谷镰刀菌拮抗菌21-1的发酵条件及稳定性分析[J].江苏农业科学,2023,51(20):122.
 Zhang Qiang,et al.Fermentation conditions and stability of antagonistic actinomycete 21-1 against Fusarium graminearum[J].Jiangsu Agricultural Sciences,2023,51(08):122.

备注/Memo

备注/Memo:
收稿日期:2019-01-20
基金项目:江苏省农业科技自主创新资金[编号:CX(17)3018];江苏省海洋与渔业科技创新与推广项目(编号:Y2017-28);江苏省六大人才高峰项目(编号:YY-006)。
作者简介:曹坤(1995—),男,江苏泰州人,主要从事微生物代谢研究。E-mail:15298667707@163.com。
通信作者:罗楚平,博士,教授,主要从事微生物代谢研究。E-mail:luochuping@163.com。
更新日期/Last Update: 2019-04-20