|本期目录/Table of Contents|

[1]翟雪,张栩,连光倩,等.水稻穗型相关性状的QTLs定位[J].江苏农业科学,2019,47(13):59-63.
 Zhai Xue,et al.QTLs localization of panicle type related traits in rice[J].Jiangsu Agricultural Sciences,2019,47(13):59-63.
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第47卷
期数:
2019年第13期
页码:
59-63
栏目:
生物技术
出版日期:
2019-07-31

文章信息/Info

Title:
QTLs localization of panicle type related traits in rice
作者:
翟雪 张栩 连光倩 王建飞
南京农业大学作物遗传与种质创新国家重点实验室,江苏南京 210095
Author(s):
Zhai Xueet al
关键词:
水稻穗型相关性状QTL定位
Keywords:
-
分类号:
S511.03
DOI:
-
文献标志码:
A
摘要:
稻穗大小是决定水稻产量的重要性状,为了挖掘与穗型相关的基因,为水稻产量遗传育种研究提供理论基础和应用价值,以大穗籼稻地方品种宝大粒(BDL)和小穗粳稻品种矮格拉(AGL)为亲本,构建F2代群体,对穗型性状进行数量性状座位(QTL)初步分析;根据F2群体定位结果,标记选择区间杂合体,自交构建F4分离群体,验证QTL位点。结果表明,在F2群体中共检测到与5个穗型相关性状的6个QTLs,分别位于第2、6、7染色体上。二次枝梗数QTL qNSB7和一次枝梗数QTL qNPB7定位于第7染色体的RM542~A83区间,LOD值(表示连锁可能性的大小)分别为10.84和5.03,分别解释30.74%和12.34%的表型变异,并且在其相邻的A85~A28和A83~A85区间分别检测到每穗颖花数QTL qSNP7和穗长QTL qPL7,LOD值分别为5.31和4.12,表型贡献率分别为14.80%和11.82%。另外,在第2染色体上检测到1个穗长QTL qPL2,LOD值为3.76,表型贡献率为9.12%;在第6染色体上检测到1个结实率QTL qSSR6,LOD值为4.34,表型贡献率为11.26%。以qNSB7为目标QTL,在F4分离群体中进行验证,结果表明,qNSB7仍位于RM542和A83标记之间,LOD值为9.84,解释了30.74%的表型变异,且其他3个QTLs qSNP7、qPL7和qNPB7都聚集在这317 kb的区间,解释了17.2%~24.6%的表型变异。由结果可知,qNSB7是1个通过增加二次枝梗数,影响每穗颖花数、一次枝梗数和穗长的一因多效新位点。
Abstract:
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参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:2018-05-28
基金项目:江苏省自然科学基金(编号:BK20151427)。
作者简介:翟雪(1991—),女,河南开封人,硕士研究生,主要研究方向为水稻遗传育种。E-mail:zhaixuezxzy@126.com。
通信作者:王建飞,博士,教授,博士生导师,从事水稻遗传育种研究。E-mail:wangjf@njau.edu.cn。
更新日期/Last Update: 2019-07-05