|本期目录/Table of Contents|

[1]曹辉庆,黄诚梅,蒋胜理,等.甘蔗SPSB基因5′侧翼序列的克隆及生物信息学分析[J].江苏农业科学,2019,47(15):94-98.
　Cao Huiqing,et al.Cloning and bioinformatics analysis of 5′-flanking sequence of SPSB gene in sugarcane[J].Jiangsu Agricultural Sciences,2019,47(15):94-98.
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甘蔗SPSB基因5′侧翼序列的克隆及生物信息学分析(PDF)
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第47卷
期数:
2019年第15期
页码:
94-98
栏目:
生物技术
出版日期:
2019-09-03

文章信息/Info

Title:
Cloning and bioinformatics analysis of 5′-flanking sequence of SPSB gene in sugarcane
作者:
曹辉庆1;  黄诚梅1;  蒋胜理1;  罗海斌1;  邓智年2;  吴凯朝2;  徐林2;  魏源文1
1.广西作物遗传改良生物技术重点开放实验室，广西南宁 530007；2.广西农业科学院甘蔗研究所，广西南宁 530007
Author(s):
Cao Huiqing; et al
关键词:
甘蔗;  ScSPSB基因; 侧翼序列; 生物信息学分析
Keywords:
-
分类号:
S566.101
DOI:
-
文献标志码:
A
摘要:
蔗糖磷酸合成酶(SPS)是蔗糖合成途径的关键限速酶，对蔗糖的合成和碳水化合物的分配具有重要的调控作用。采用染色体步移法技术克隆获得了甘蔗SPSB基因5′端侧翼序列，并对其序列进行生物信息学分析。分离获得了4 399 bp的5′侧翼序列，该序列除具有启动子核心序列 TATA-box 和增强子元件 CAAT-box等典型的真核生物启动子基本元件外，还含有多种光应答元件、激素响应元件，以及与缺氧、干旱、低温诱导有关的顺式作用元件和一些组织调控表达有关的顺式调控元件，表明ScSPSB启动子可能具有逆境应答、激素应答及组织特异表达特性，值得开展进一步的功能研究。
Abstract:
-

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备注/Memo

备注/Memo:
收稿日期：2019-05-05
基金项目：广西农业科学院基本科研业务专项(编号：桂农科2017YM35、2015YT96)。
作者简介：曹辉庆(1975—)，女，广西全州人，硕士，助理研究员，主要从事作物栽培学与分子生物学研究工作。E-mail：1034403583@qq.com。
通信作者：魏源文，博士，研究员，主要从事植物分子生物学与分子病理学研究工作。E-mail：yw-wei@126.com。
更新日期/Last Update: 2019-08-05