|本期目录/Table of Contents|

[1]范素菊,李嘉,李森,等.金刚烷胺单克隆抗体的制备和鉴定[J].江苏农业科学,2019,47(15):220-223,232.
　Fan Suju,et al.Preparation and identification of monoclonal antibodies against amantadine[J].Jiangsu Agricultural Sciences,2019,47(15):220-223,232.
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金刚烷胺单克隆抗体的制备和鉴定(PDF)
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第47卷
期数:
2019年第15期
页码:
220-223,232
栏目:
贮藏加工与检测分析
出版日期:
2019-09-03

文章信息/Info

Title:
Preparation and identification of monoclonal antibodies against amantadine
作者:
范素菊1; 2;  李嘉1;  李森1;  杨兴东2
1.周口职业技术学院农牧工程学院，河南周口 466000； 2.周口师范学院食品药品检验检测中心，河南周口 466001
Author(s):
Fan Suju; et al
关键词:
金刚烷胺; 免疫原; 单克隆抗体; ELISA; 抗体制备; 抗体鉴定
Keywords:
-
分类号:
S852.4+3
DOI:
-
文献标志码:
A
摘要:
为制备金刚烷胺(AMD)的单克隆抗体(mAb)，改造AMD的半抗原，采用EDC/NHS法合成人工免疫原AMD-BSA和检测抗原AMD-OVA，经紫外扫描和凝胶电泳进行鉴定，AMD与载体蛋白［牛血清白蛋白(BSA)、鸡卵白蛋白(OVA)］成功偶联；用被免疫原(AMD-BSA)免疫BALBC/c小鼠的脾细胞与骨髓瘤细胞进行融合，获得AMD杂交瘤细胞株，腹腔注射小鼠后，得到抗AMD的mAb。小鼠免疫后的多抗血清的效价均在6.4×103以上，所制得的杂交瘤细胞上清液的效价为3.6×102~1.0×103，2D5细胞株的腹水效价为5.12×105，对AMD的IC50值为1.02 μg/L，除与金刚乙胺的交叉反应率为9.82%，与其他结构类似物无交叉反应性。表明获得了高价、敏感和特异的抗AMD的单克隆抗体，为进一步的免疫学快速检测提供了技术支持。
Abstract:
-

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期：2019-03-23
基金项目：河南省自然科学基金(编号：182300410093)；河南省科技攻关项目(编号：182102310683)。
作者简介：范素菊(1981—)，女，河南商水人，硕士，讲师，主要从事兽药残留的免疫学快速检测研究。E-mail：420721901@qq.com。
通信作者：杨兴东，博士，讲师，主要从事食品免疫学快速检测研究。E-mail：zzyangxd@163.com。
更新日期/Last Update: 2019-08-05