|本期目录/Table of Contents|

[1]余雪梅,彭书明,王洪婷,等.耐镉芽孢杆菌对Cd2+的吸附机制[J].江苏农业科学,2019,47(20):293-297.
 Yu Xuemei,et al.Mechanism of Cd(Ⅱ) biosorption by cadmium-tolerant Bacillus sp.[J].Jiangsu Agricultural Sciences,2019,47(20):293-297.
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耐镉芽孢杆菌对Cd2+的吸附机制(PDF)
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第47卷
期数:
2019年第20期
页码:
293-297
栏目:
资源与环境
出版日期:
2019-11-18

文章信息/Info

Title:
Mechanism of Cd(Ⅱ) biosorption by cadmium-tolerant Bacillus sp.
作者:
余雪梅 彭书明 王洪婷 伏媛 李璟 张山
成都理工大学环境学院/国家环境保护水土污染协同控制与联合修复重点实验室,四川成都 611059
Author(s):
Yu Xuemeiet al
关键词:
镉污染耐镉细菌驯化生物吸附吸附机理
Keywords:
-
分类号:
X703.1
DOI:
-
文献标志码:
A
摘要:
从攀枝花矿区淤泥样品中通过逐级提高Cd2+浓度进行驯化,获得1株高耐镉菌株PFYN01,经16S rDNA PCR鉴定为芽孢杆菌(Bacillus sp),最大耐Cd2+浓度为3 900 mg/L。研究初始Cd2+浓度、pH值、菌量对菌株吸附Cd2+的影响,利用扫描电镜(SEM)和傅叶里红外光谱(FTIR)探究菌株吸附机制。结果表明,PFYN01在初始Cd2+浓度为75 mg/L、菌量为1.0 g/L、pH值为5.0时,对Cd2+的吸附率达到34.98%;吸附符合Langmuir模型,最大吸附量为 1.974 mg/g。对比分析Cd2+吸附前后的细胞形态和红外光谱变化,证实了“细胞成分羟基(O—H)、酰胺基(N—H)、烃基(C—H)、羧基(C=O)、羰基(—COOH)参与了Cd2+与PFYN01的相互作用”的结论。PFYN01是一株对Cd2+有较强吸附能力的细菌菌株,对其吸附Cd2+影响因素及吸附机制研究的结果将为重金属污染微生物修复提供指导。
Abstract:
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参考文献/References:

[1]Klimek B. Effect of long-term zinc pollution on soil microbial community resistance to repeated contamination[J]. Bulletin of Environmental Contamination and Toxicology,2012,88(4):617-622.
[2]Kumar N,Kumari V,Ram C,et al. Bio-prospectus of cadmium bioadsorption by lactic acid bacteria to mitigate health and environmental impacts[J]. Applied Microbiology and Biotechnology,2018,102(4):1599-1615.
[3]Nies D H. Microbial heavy-metal resistance[J]. Applied Microbiology & Biotechnology,1999,51(6):730-750.
[4]林晓燕,牟仁祥,曹赵云,等. 耐镉细菌菌株的分离及其吸附镉机理研究[J]. 农业环境科学学报,2015,34(9):1700-1706.
[5]Amoozegar M A,Ghazanfari N,Didari M. Lead and cadmium bioremoval by Halomonas sp. an exopolysaccharide-producing Halophilic bacterium[J]. Progress in Biological Sciences,2012,2(1):1-11.
[6]廖佳,冯冲凌,李科林,等. 耐性真菌HA吸附铅、锌的影响因素及吸附机理研究[J]. 微生物学通报,2015,42(2):254-263.
[7]Samanta A,Bera P,Khatun M,et al. An investigation on heavy metal tolerance and antibiotic resistance properties of bacterial strain Bacillus sp.isolated from municipal waste[J]. Journal of Microbiology & Biotechnology Research,2017,2(1):178-189.
[8]Kim S U,Cheong Y H,Seo D C,et al. Characterisation of heavy metal tolerance and biosorption capacity of bacterium strain CPB4 (Bacillus spp.)[J]. Water Science and Technology,2007,55(1/2):105-111.
[9]Wang T,Sun H W,Mao H J,et al. The immobilization of heavy metals in soil by bioaugmentation of a UV-mutant Bacillus subtilis 38 assisted by NovoGro biostimulation and changes of soil microbial community[J]. Journal of Hazardous Materials,2014,278:483-490.
[10]刘红娟,党志,张慧,等. 蜡状芽孢杆菌抗重金属性能及对镉的累积[J]. 农业环境科学学报,2010,29(1):25-29.
[11]Tripathi M,Vikram S,Jain R K,et al. Isolation and growth characteristics of chromium(Ⅵ) and pentachlorophenol tolerant bacterial isolate from treated tannery effluent for its possible use in simultaneous bioremediation[J]. Indian Journal of Microbiology,2011,51(1):61-69.
[12]沈秋悦,曹志强,朱月芳,等. 一株耐镉细菌的分离鉴定及其吸附条件的优化[J]. 土壤,2016,48(3):615-620.
[13]Gabr R M,Hassan S H A,Shoreit A A M. Biosorption of lead and nickel by living and non-living cells of Pseudomonas aeruginosa ASU 6a[J]. International Biodeterioration & Biodegradation,2008,62(2):195-203.
[14]Yang Y T,Wei X Z,Wan J M,et al. Equilibrium and kinetic characteristic of adsorption of Cu2+,Pb2+ on a novel anionic starch microspheres[J]. Water,Air,& Soil Pollution,2011,219(1/2/3/4):103-112.
[15]周赓,杨辉,潘虎,等. 一株耐镉链霉菌的筛选、鉴定与基本特性分析[J]. 环境科学学报,2017,37(6):2076-2084.
[16]李新琦,温沁雪,王琼,等. 处理电镀废水的耐铜功能菌的筛选及吸附性能[J]. 哈尔滨工业大学学报,2016,48(8):108-114.
[17]卢涌泉,邓振华. 实用红外光谱解析[M]. 北京:电子工业出版社,1989:203-207.
[18]Kim S Y,Jin M R,Chung C H,et al. Biosorption of cationic basic dye and cadmium by the novel biosorbent Bacillus catenulatus JB-022 strain[J]. Journal of Bioscience and Bioengineering,2015,119(4):433-439.
[19]Sun J,Li Y,Liu L G,et al. Bioaccumulation capacity and mechanisms of cadmium by Bacillus Licheniformis[J]. Journal of Nuclear Agricultural Sciences,2013,27(11):1644-1651.
[20]Fang L C,Cai P,Chen W L,et al. Impact of cell wall structure on the behavior of bacterial cells in the binding of copper and cadmium[J]. Colloids and Surfaces (A:Physicochemical and Engineering Aspects),2009,347(1/2/3):50-55.
[21]陈永华,向捷,吴晓芙,等. 三株耐铅锌菌的分离、鉴定及其吸附能力[J]. 生态学杂志,2015,34(9):2665-2672.
[22]黄飞. 蜡状芽孢杆菌对水体中镉的吸附特性与机理研究[D]. 广州:华南理工大学,2013:117-118.

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备注/Memo

备注/Memo:
收稿日期:2018-07-10
基金项目:四川省科技计划(编号:2017SZ0185);科技部国际科技合作计划(编号:2013DFA21690);国家自然科学基金面上项目(编号:3137070605)。
作者简介:余雪梅(1991—),女,四川成都人,硕士研究生,研究方向为矿山环境生态治理。E-mail:644483798@qq.com。
通信作者:彭书明,博士,副教授,研究方向为水土介质污染恢复与治理、退化生态系统恢复与治理研究。E-mail:pengshuming06@cdut.cn。
更新日期/Last Update: 2019-10-20