|本期目录/Table of Contents|

[1]赵许朋,刘勇,魏加练,等.贵长猕猴桃茎段高效再生体系的建立[J].江苏农业科学,2019,47(22):43-46.
 Zhao Xupeng,et al.Establishment of highly efficient regeneration system of stem segments of “Guichang” kiwifruit[J].Jiangsu Agricultural Sciences,2019,47(22):43-46.
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贵长猕猴桃茎段高效再生体系的建立(PDF)
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第47卷
期数:
2019年第22期
页码:
43-46
栏目:
生物技术
出版日期:
2019-12-20

文章信息/Info

Title:
Establishment of highly efficient regeneration system of stem segments of “Guichang” kiwifruit
作者:
赵许朋 刘勇 魏加练 黄杰城 韩立端 刘燕
贵阳学院,贵州贵阳 550005
Author(s):
Zhao Xupenget al
关键词:
贵长猕猴桃茎段增殖植株再生
Keywords:
-
分类号:
S663.404+.3
DOI:
-
文献标志码:
A
摘要:
以贵长猕猴桃茎段为外植体,研究不同激素组合培养基对愈伤组织诱导、不定芽分化增殖、无菌苗生根等的影响。结果表明,贵长猕猴桃茎段在MS+2,4-D 2.0 mg/L+6-BA 0.4 mg/L+蔗糖30 g/L+琼脂粉8 g/L培养基上愈伤组织诱导率相对最高,达100.0%,且愈伤组织生长状况相对较好,质地最优,呈黄白色湿润致密状,在MS+6-BA 5 mg/L+NAA 0.2 mg/L+蔗糖30 g/L+琼脂粉8 g/L培养基上不定芽出芽率相对最高,达83.33%,在MS+6-BA 4.0 mg/L+NAA 0.5 mg/L+GA3 0.4 mg/L+蔗糖30 g/L+琼脂粉8 g/L培养基上不定芽增殖率相对最高,达100%,继代培养6代时的平均繁殖系数达6.48;不定芽在含IBA 0.6 mg/L的1/2MS培养基上培养生根率相对最高,达到95.83%,且形成庞大的根系,50株试管苗经炼苗移栽成活率可达96.0%。
Abstract:
-

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备注/Memo

备注/Memo:
收稿日期:2018-07-26
基金项目:贵州省教育厅重点项目(编号:黔教合KY字[2015]382号);贵州省科技厅联合基金(编号:黔科合LH字[2015]7310号,黔科合LH字[2014]7168号);贵州省大学生创新创业训练计划一般项目(编号:201710976110);贵州省一流大学建设项目(编号:2017158322)。
作者简介:赵许朋(1982—),男,博士,从事植物生物技术研究。E-mail:zhaoxupeng666@163.com。
通信作者:刘燕,教授,从事生物资源利用及环境生态研究。E-mail:gyly68@sina.com。
更新日期/Last Update: 2019-11-20