|本期目录/Table of Contents|

[1]张国壁,马静,左壮,等.转PnAlaAT3基因可提高低氮条件下杨树上位叶谷氨酰胺合成酶活力[J].江苏农业科学,2020,48(03):86-90.
　Zhang Guobi,et al.Glutamine synthetase activity in upper leaves of transgenic PnAlaAT poplar under low nitrogen conditions[J].Jiangsu Agricultural Sciences,2020,48(03):86-90.
点击复制

转PnAlaAT3基因可提高低氮条件下
杨树上位叶谷氨酰胺合成酶活力(PDF)
分享到：

《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第48卷
期数:
2020年第03期
页码:
86-90
栏目:
生物技术
出版日期:
2020-03-15

文章信息/Info

Title:
Glutamine synthetase activity in upper leaves of transgenic PnAlaAT poplar under low nitrogen conditions
作者:
张国壁;  马静;  左壮;  许志茹;  杨成君;  刘关君;  曲春浦
东北林业大学，黑龙江哈尔滨 150040
Author(s):
Zhang Guobi; et al
关键词:
谷丙转氨酶基因; 杨树; 转基因; 氮素同化; 谷氨酰胺合成酶活性
Keywords:
-
分类号:
Q785
DOI:
-
文献标志码:
A
摘要:
谷氨酸氨基转移酶又称谷丙转氨酶，在植物氮同化中起到关键作用。本试验以小黑杨为试材构建pROKⅡ-PnAlaAT3植物表达载体，通过农杆菌介导法转化小黑杨，获得转基因植株，发现5号转基因株系叶片中PnAlaAT3基因表达量升高，但根中表达量却显著降低。对野生型和PnAlaAT3 5号转基因株系的谷氨酰胺合成酶（GS）和谷氨酰胺-α-酮戊二酸氨基转移酶（GOGAT）活性检测结果发现，在低氮条件下，PnAlaAT3转基因株系上位叶GS活LT 较野生型植株有显著增加。结果表明，PnAlaAT3基因可以提高小黑杨上位叶中GS活力。
Abstract:
-

参考文献/References:

［1］Miyashita Y，Dolferus R，Ismond K P，et al. Alanine aminotransferase catalyses the breakdown of alanine after hypoxia in Arabidopsis thaliana［J］. The Plant Journal，2007，49(6)：1108-1121.
［2］Yonaha K，Suzuki K，Toyama S. Streptomyces beta-alanine：alpha-ketoglutarate aminotransferase，a novel omega-amino acid transaminase. Purification，crystallization，and enzymologic properties［J］. Journal of Biological Chemistry，1985，260：3265-3268.
［3］黄冰艳，高伟，苗利娟，等. 谷氨酰胺合成酶基因研究进展及其在植物氮代谢调控中的应用［J］. 中国农学通报，2010，26(23)：53-57.
［4］Muench D G，Christopher M E，Good A G. Cloning and expression of a hypoxic and nitrogen inducible maize alanine aminotransferase gene［J］. Physiologia Plantarum，1998，103(4)：503-512.
［5］Igarashi D，Miwa T，Seki M，et al. Identification of photorespiratory glutamate：glyoxylate aminotransferase (GGAT) gene in Arabidopsis［J］. The Plant Journal，2003，33(6)：975-987.
［6］Rocha M，Sodek L，Licausi F，et al. Analysis of alanine aminotransferase in various organs of soybean (Glycine max) and in dependence of different nitrogen fertilisers during hypoxic stress［J］. Amino Acids，2010，39(4)：1043-1053.
［7］Ricoult C，Echeverria L O，Cliquet J B，et al. Characterization of alanine aminotransferase (AlaAT) multigene family and hypoxic response in young seedlings of the model legume Medicago truncatula［J］. Journal of Experimental Botany，2006，57(12)：3079-3089.
［8］Shrawat A K，Carroll R T，DePauw M，et al. Genetic engineering of improved nitrogen use efficiency in rice by the tissue-specific expression of alanine aminotransferase［J］. Plant Biotechnology Journal，2008，6(7)：722-732.
［9］Good A G，Johnson S J，De Pauw M，et al. Engineering nitrogen use efficiency with alanine aminotransferase［J］. Revue Canadienne De Botanique，2007，85：252-262.
［10］Snyman S J，Hajari E，Watt M P，et al. Improved nitrogen use efficiency in transgenic sugarcane：phenotypic assessment in a pot trial under low nitrogen conditions［J］. Plant Cell Reports，2015，34(5)：667-669.
［11］徐红卫，郭桂梅，黄赛华， 等. 两份大麦纯合突变体氮代谢相关基因于不同生长发育时期的表达［J］. 植物生理学报，2017，53(11)：2031-2036.
［12］Xu Z，Jing M，Qu C，et al. Identification and expression analyses of the alanine aminotransferase(AlaAT) gene family in poplar seedlings［J］. Scientific Reports，2017，7：45933.
［13］Enosawa S，Dozen M，Tada Y，et al. Electron therapy attenuated elevated alanine aminotransferase and oxidative stress values in type 2 diabetes-induced nonalcoholic steatohepatitis of rats［J］. Cell Medicine，2013，6(1/2)：63-73.
［14］Tian H，Fu J，Drijber RA，et al. Expression patterns of five genes involved in nitrogen metabolism in two winter wheat(Triticum aestivum L.) genotypes with high and low nitrogen utilization efficiencies［J］. Journal of Cereal Science，2015，61：48-54.
［15］董召娣，易媛，张明伟，等. 春性和半冬性小麦花后旗叶和籽粒氮代谢关键酶活性的差异［J］. 麦类作物学报，2015，35(8)：1098-1106.
［16］Mauchline T H，Fowler J E，East A K，et al. Mapping the Sinorhizobium meliloti 1021 solute-binding protein-dependent transportome［J］. Proceedings of the National Academy of Sciences of the United States of America，2006，103(47)：17933-17938.
［17］Kan C C，Chung T Y，Juo Y A，et al. Glutamine rapidly induces the expression of key transcription factor genes involved in nitrogen and stress responses in rice roots［J］. BMC Genomics，2015，16(1)：731.

相似文献/References:

[1]刘志祥,曾超珍,周永青,等.杨树MIR156基因家族的进化与功能分化[J].江苏农业科学,2014,42(01):26.
　Liu Zhixiang,et al.Evolution and functional diversification of poplar MIR156 gene family[J].Jiangsu Agricultural Sciences,2014,42(03):26.
[2]姚丽,禹婷,秦刚,等.转双抗虫基因741杨树对节肢动物群落食物网的影响[J].江苏农业科学,2014,42(01):299.
　Yao Li,et al.Effect of transgenic hybrid poplar 741 with two insect-resistant genes on food web of arthropod animal community[J].Jiangsu Agricultural Sciences,2014,42(03):299.
[3]李杰,雒雅婧,张爽,等.杨树木质部特异启动子诱导活性分析[J].江苏农业科学,2016,44(01):63.
　Li Jie,et al.Analysis of induction activity of xylem specific promoter of Populus tremula[J].Jiangsu Agricultural Sciences,2016,44(03):63.
[4]吴雅静,王雯雯,田兴军.不同灯光和培养方式对柳树、杨树插条生长的影响[J].江苏农业科学,2015,43(11):256.
　Wu Yajing,et al.Effects of light and culture method on growth of willow and poplar[J].Jiangsu Agricultural Sciences,2015,43(03):256.
[5]李慧,李爱,彭立新,等.杨树EBP1基因的克隆与表达载体构建[J].江苏农业科学,2015,43(04):14.
　Li Hui,et al.Cloning of EBP1 gene from populus and construction of its plant expression vector[J].Jiangsu Agricultural Sciences,2015,43(03):14.
[6]主楚杰,王爱云.基因工程技术在杨树抗逆境方面的研究进展[J].江苏农业科学,2015,43(02):10.
　Zhu Chujie,et al.Research progress of anti-adversity of poplar by gene engineering[J].Jiangsu Agricultural Sciences,2015,43(03):10.
[7]贾新社,袁文丽.萘乙酸·吲哚丁酸促进杨树生根试验[J].江苏农业科学,2017,45(04):96.
　Jia Xinshe,et al.Induction of poplar rooting by naphthylacetic acid·indolebutyric acid (NAA· IBA)[J].Jiangsu Agricultural Sciences,2017,45(03):96.
[8]王沛雅,杨晖,郭琪,等.分子遗传改良技术在杨树上的研究与应用进展[J].江苏农业科学,2017,45(20):17.
　Wang Peiya,et al.Research and application progress of molecular genetic improvement technology in poplar[J].Jiangsu Agricultural Sciences,2017,45(03):17.
[9]吴宝成,韦敏,宋春凤,等.江苏地区杨树林下套种细柱五加栽培模式[J].江苏农业科学,2017,45(23):156.
　Wu Baocheng,et al.Study on cultivation model of populus intercropping Eleutherococcus nodiflorus in Jiangsu Province[J].Jiangsu Agricultural Sciences,2017,45(03):156.
[10]马亚峰,侯银,张焕朝.杨树不同林分密度和林分结构对土壤理化性质的影响[J].江苏农业科学,2018,46(22):131.
　Ma Yafeng,et al.Effects of different stand structures of poplar on soil physical and chemical properties[J].Jiangsu Agricultural Sciences,2018,46(03):131.

备注/Memo

备注/Memo:
收稿日期：2019-01-06
基金项目：国家自然科学基金青年科学基金(编号：31600534)；国家自然科学基金面上项目(编号：31570648)；中央高校基础科研业务费(编号：DL13EA03-01)。
作者简介：张国壁(1994—)，男，山东聊城人，硕士，主要从事氮素营养与林木生长发育。E-mail：2456526675@qq.com。
通信作者：曲春浦，博士，工程师，主要从事氮素营养与林木生长发育。E-mail：qcp_0451@163.com。
更新日期/Last Update: 2020-02-05