|本期目录/Table of Contents|

[1]肖金星,王磊,郑刚,等.硒化多糖对黑鲷存活、免疫力和抗氧化性能的影响[J].江苏农业科学,2020,48(09):196-201.
　Xiao Jinxing,et al.Influences of dietary selenium polysaccharide on survival rate，immunity and oxidation resistance of juvenile black sea bream，Acanthopagrus schlegelii[J].Jiangsu Agricultural Sciences,2020,48(09):196-201.
点击复制

硒化多糖对黑鲷存活、免疫力和抗氧化性能的影响(PDF)
分享到：

《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第48卷
期数:
2020年第09期
页码:
196-201
栏目:
畜牧兽医与水产蚕桑
出版日期:
2020-05-05

文章信息/Info

Title:
Influences of dietary selenium polysaccharide on survival rate，immunity and oxidation resistance of juvenile black sea bream，Acanthopagrus schlegelii
作者:
肖金星1;  王磊2;  郑刚1;  周凡4;  周宇芳3
1.浙江大学舟山海洋研究中心，浙江舟山 316021； 2.浙江大学动物科学学院，浙江杭州 310058；
3.浙江省海洋开发研究院，浙江舟山 316021； 4.浙江省水产技术推广总站，浙江杭州 310012
Author(s):
Xiao Jinxing; et al
关键词:
黑鲷; 硒化多糖; 存活率; 免疫力; 抗氧化性能
Keywords:
-
分类号:
S965.231
DOI:
-
文献标志码:
A
摘要:
为了探究饲料中添加硒化多糖对攻毒试验后黑鲷存活、免疫力和抗氧化性能的影响，选用初始体质量为(13.00±0.20) g黑鲷幼鱼随机分为6组，分别投喂硒含量为0.34、0.52、0.68、0.91、1.08、3.06 mg/kg的6组等能等氮试验饲料D1~D6，开展为期8周的室内微流水养殖。之后每缸随机选取10尾黑鲷，腹腔注射副溶血弧菌，开展为期10 d攻毒试验。结果显示，黑鲷血清溶菌酶(LZM)、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和谷胱甘肽过氧化物酶(GPx)的活性随着饲料硒含量的增加而显著升高(P<0.05)，而累积死亡率和血清溶菌酶(MDA)含量随着饲料硒含量的增减而显著降低(P<0.05)，当饲料硒含量超过0.91 mg/kg时，这些指标趋于平稳。以副溶血弧菌感染后黑鲷的免疫力、抗氧化性能和存活率为评价指标，黑鲷对饲料中硒的最适需求量均为0.91 mg/kg。
Abstract:
-

参考文献/References:

［1］National Research Council. Nutrient requirements of fish［M］. Washington DC，USA：National Academy Press，1993：144.
［2］Lin Y H，Shiau S Y. Dietary selenium requirements of juvenile grouper，Epinephelus malabaricus［J］. Aquaculture，2005，250：356-363.
［3］Zhou X，Wang Y，Gu Q，et al. Effects of different dietary selenium sources (selenium nanoparticle and selenomethionine) on growth performance，muscle composition and glutathione peroxidase enzyme activity of crucian carp (Carassius auratus gibelio)［J］. Aquaculture，2009，291：78-81.
［4］Saffari S，Keyvanshokooh S，Zakeri M，et al. Effects of dietary organic，inorganic，and nanoparticulate selenium sources on growth，hemato-immunological，and serum biochemical parameters of common carp (Cyprinus carpio)［J］. Fish Physiol Biochem，2018，44：1087-1097.
［5］Hamilton S J. Review of selenium toxicity in the aquatic food chain［J］. Science of the Total Environment，2004，326(1/2/3)：1-31.
［6］Atencio L，Moreno I，?ngeles J，et al. Effects of dietary selenium on the oxidative stress and pathological changes in tilapia(Oreochromis niloticus)exposed to a microcystin-producing cyanobacterial water bloom［J］. Toxicon，2009，53(2)：269-282.
［7］Saleh R，Betancor M B，Roo J，et al. Selenium levels in early weaning diets for gilthead seabream larvae［J］. Aquaculture，2014，426-427：256-263.
［8］Rayman M P. The importance of selenium to human health［J］. The Lancet，2000，356(9225)：233-241.
［9］Küükbay F Z，Yazlak H，Karaca I，et al. The effects of dietary organic or inorganic selenium in rainbow trout(Oncorhynchus mykiss)under crowding conditions［J］. Aquaculture Nutrition，2009，15(6)：569-576.
［10］Lin，Y H. Effects of dietary organic and inorganic selenium on the growth，selenium concentration and meat quality of juvenile grouper Epinephelus malabaricus［J］. Aquaculture，2014，430：114-119.
［11］Wang L，Wang G，Zhang J，et al. Extraction optimization and antioxidant activity of intracellular selenium polysaccharide by Cordyceps sinensis SU-02［J］. Carbohydrate Polymers，2011，86(4)：1745-1750.
［12］Wang J，Zhao B，Wang X，et al. Synthesis of selenium-containing polysaccharides and evaluation of antioxidant activity in vitro［J］. International Journal of Biological Macromolecules，2012，51：987-991.
［13］Malinowska E，Krzyczkowski W，Herold F A，et al. Biosynthesis of selenium-containing polysaccharides with antioxidant activity in liquid culture of Hericium erinaceum［J］. Enzyme and Microbial Technology，2009，44(5)：334-343.
［14］Nip T H M，Ho W Y，Wong C K. Feeding ecology of larval and juvenile black seabream (Acanthopagrus schlegeli) and Japanese seaperch (Lateolabrax japonicus) in Tolo Harbour，Hong Kong［J］. Environmental Biology of Fishes，2003，66 (2)：197-209.
［15］Shao Q，Ma J，Xu Z，et al. Dietary phosphorus requirement of juvenile Black Sea bream，Sparus macrocephalus［J］. Aquaculture，2008，277(1)：92-100.
［16］胡王龙，邵庆均，王秀英. 池塘养殖黑鲷常见疾病的防治［J］. 中国水产，2004(5)：51-52.
［17］李莉，李春梅. 鱼类非特异性免疫研究进展［J］. 河南农业科学，2012，41(2)：26-32.
［18］Mori K，Nakanishi T，Suzuki T，et al. Defense mechanisms in invertebrates and fish［J］. Tanpakushitsu Kakusan Koso，1989，34(3)：214-223.
［19］Boman H G，Faye I，Gudmundsson G H，et al. Cell-free immunity in Cecropia.A model system for antibacterial proteins［J］. European Journal of Biochemistry，1991，201(1)：23-31.
［20］陈兴发. 姜黄素对罗非鱼生长、抗氧化及脂类代谢的影响［D］. 广州：华南农业大学，2008.
［21］宋晓玲，杨绪彤，思瀚文，等. 双歧杆菌细胞壁肽聚糖的分离及其对二种海产动物免疫活性的影响［J］. 水产学报，2005，29(3)：350-355.
［22］郭玉娟，陈学年. A3α肽聚糖对彭泽鲫生长及非特异性免疫机能的影响［J］. 水产科学，2006，25(2)：65-68.
［23］Sheffy B E，Schultz R D. Influence of vitamin E and selenium on immune response mechanisms［J］. Federation Proceedings，1979，38(7)：2139-2143.
［24］Low K W，Sin Y M. In vivoandin vitroeffects of mercuric chloride and sodium selenite on some non-specific immune responses of blue gourami，Trichogaster trichopterus(Pallus)［J］. Fish & Shellfish Immunology，1996，6(5)：351-362.
［25］Hafeman D G，Hoekstra W G. Protection against carbon Tetrachloride-Induced lipid peroxidation in the rat by dietary vitamin E，selenium，and methionine as measured by ethane evolution［J］. The Journal of Nutrition，1977，107(4)：656-665.
［26］Omaye S T，Reddy K A，Cross C E. Enhanced lung toxicity of paraquat in selenium-deficient rats［J］. Toxicology and Applied Pharmacology，1978，43(2)：237-247.
［27］Amstad P，Moret R，Cerutti P. Glutathione peroxidase compensates for the hypersensitivity of Cu，Zn-superoxide dismutase overproducers to oxidant stress［J］. Journal of Biological Chemistry，1994，269(3)：1606-1609.
［28］Gutteridge J C. Superoxide dismutase inhibits the superoxidedriven Fenton reaction at two different levels：Implication for a wider protective role［J］. FEBS Letters，1985，185(1)：19-23.
［29］Schrader M，Fahimi H D. Peroxisomes and oxidative stress［J］. Biochimica et Biophysica Acta-Molecular Cell Research，2006，1763(12)：1755-1766.
［30］Mills G C. Hemoglobin catabolism. Ⅰ. Glutathione peroxidase，an erythrocyte enzyme which protects hemoglobin from oxidative breakdown［J］. The Journal of Biological Chemistry，1957，229(1)：189-197.
［31］Gebhardt R. Oxidative stress，Plant-Derived antioxidants and liver fibrosis［J］. Planta Medica，2002，68(4)：289-296.
［32］Combs G F. The role of selenium in nutrition［J］. Quarterly Review of Biology，1987，46(1)：124-125.
［33］Wang Y B，Han J Z，Li W F. Effect of different selenium source on growth performances，glutathione peroxidase activities，muscle composition and selenium concentration of allogynogenetic crucian carp (Carassius auratus gibelio)［J］. Anim Feed Sci Tech，2007，134：243-251.
［34］Ashouri S，Keyvanshokooh S，Salati A P，et al. Effects of different levels of dietary selenium nanoparticles on growth performance，muscle composition，blood biochemical profiles and antioxidant status of common carp (Cyprinus carpio)［J］. Aquaculture，2015，446：25-29.
［35］Esterbauer H，Schaur R J，Zollner H. Chemistru and biochemistry of 4-hydroxynonenal，malonaldehyde and related aldehydes［J］. Free Radical Biology and Medicine，1991，11(1)：81-128.
［36］Burhan A，Ibrahim O，Zeliha S T，et al. Effects of sodium selenite on some biochemical and hematological parameters of rainbow trout (Oncorhynchus mykiss Walbaum，1792) exposed to Pb2+and Cu2+［J］. Fish Physiology and Biochemistry，2008，34 (1)：53-59.
［37］罗辉，周小秋. 硒与水生动物免疫功能的关系［J］. 动物营养学报，2006(S1)：378-382.
［38］Wang C，Lovell R T. Organic Selenium sources，selenomethionine and selenoyeast，have higher bioavailability than an inorganic selenium source，sodium selenite，in diets for channel catfish(Ictalurus punctatus)［J］. Aquaculture，1997，152(1)：223-234.
［39］华雪铭，周洪琪，邱小琮，等. 饲料中添加芽孢杆菌和硒酵母对异育银鲫的生长及抗病力的影响［J］. 水产学报，2001，25(5)：448-453.

相似文献/References:

[1]乔毅,沈辉,万夕和,等.1株黑鲷致病性假交替单胞菌的鉴定及毒力基因分析[J].江苏农业科学,2015,43(04):229.
　Qiao Yi,et al.Identification and virulence genes analysis of a Pseudoalteromonas strain isolated from diseased Acanthopagrus schlegelii[J].Jiangsu Agricultural Sciences,2015,43(09):229.
[2]肖金星,李广经,华颖,等.饲料碳水化合物水平对黑鲷幼鱼生长性能和血清生化指标的影响[J].江苏农业科学,2017,45(10):125.
　Xiao Jinxing,et al.Effects of dietary carbohydrate levels on growth performance and serum biochemical index of juvenile black sea bream，Acanthopagrus schlegelii[J].Jiangsu Agricultural Sciences,2017,45(09):125.
[3]王裕玉,徐钢春,张志伟,等.黑鲷营养需求与饲料研究进展[J].江苏农业科学,2020,48(03):56.
　Wang Yuyu,et al.Research progress on nutritional requirement and feed of black sea bream(Acanthopagrus schlegelii)[J].Jiangsu Agricultural Sciences,2020,48(09):56.
[4]王裕玉,徐跑,张志伟,等.不同养殖模式对黑鲷生长、血清生化指标及抗氧化性能的影响[J].江苏农业科学,2020,48(23):155.
　Wang Yuyu,et al.Effects of culture models on growth，serum biochemical indices and antioxidant properties of juvenile black sea bream(Acanthopagrus schlegelii)[J].Jiangsu Agricultural Sciences,2020,48(09):155.

备注/Memo

备注/Memo:
收稿日期：2019-11-17
基金项目：浙江省重点研发计划(编号：2019C02076)；浙江省舟山市科技计划(编号：2017C12030、2018C41027)。
作者简介：肖金星(1986—)，男，山东济南人，硕士，工程师，主要从事水产动物营与饲料研究。Tel：(0580)8171365；E-mail：xiaojinxing@yeah.net。
通信作者：周宇芳，硕士，工程师，主要从事水产品加工与利用研究。Tel：(0580)2299220；E-mail：yfzhou@
更新日期/Last Update: 2020-05-05