|本期目录/Table of Contents|

[1]李鹏.利用CRISPR/Cas9系统编辑拟南芥ILR3基因及功能验证[J].江苏农业科学,2020,48(14):78-82.
　Li Peng.Editing gene ILR3 in Arabidopsis using CRISPR/Cas9 system and its function verification[J].Jiangsu Agricultural Sciences,2020,48(14):78-82.
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利用CRISPR/Cas9系统编辑拟南芥ILR3基因
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第48卷
期数:
2020年第14期
页码:
78-82
栏目:
生物技术
出版日期:
2020-07-20

文章信息/Info

Title:
Editing gene ILR3 in Arabidopsis using CRISPR/Cas9 system and its function verification
作者:
李鹏
上海辰山植物园/中国科学院上海辰山植物科学研究中心，上海 201602
Author(s):
Li Peng
关键词:
拟南芥; AtILR3; YAO启动子;  CRISPR/Cas9; 基因编辑
Keywords:
-
分类号:
Q784
DOI:
-
文献标志码:
A
摘要:
拟南芥转录因子ILR3(IAA-Leucine Resistant 3)在铁稳态的调节、葡萄糖异硫氰酸盐(glucosinolate，简称GLS)的生物合成和病原体响应方面起到重要作用。为更深入探索该转录因子在植物体内的更多功能，利用YAO基因启动子驱动Cas9在拟南芥中表达，成功获得ILR3基因编辑突变体。测序结果及序列分析结果表明，在ILR3编辑拟南芥中，该基因编码区发生了碱基缺失或插入，导致蛋白ILR3保守结构域丢失。并且，这些基因编辑突变体T2代幼苗与T-DNA插入突变体ilr3-2在缺铁环境下表现出相同的性状，进一步验证ILR3转录因子在植物对铁的吸收及体内平衡的作用，也为深入研究其更多生物学功能奠定了工作基础。
Abstract:
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备注/Memo

备注/Memo:
收稿日期：2019-08-01
基金项目：上海市青年科技英才扬帆计划(编号：19YF1414800)。
作者简介：李鹏(1983—)，男，安徽濉溪人，硕士，工程师，主要从事植物抗逆性研究。E-mail：penglee98@163.com。
更新日期/Last Update: 2020-07-20