|本期目录/Table of Contents|

[1]罗庆,钟秀来,卢松,等.胡萝卜1个新4CL基因的克隆、进化及表达分析[J].江苏农业科学,2020,48(15):89-94.
　Luo Qing,et al.Cloning，evolution and expression of a new 4-coumarate：CoA ligase gene in carrot (Daucus carota L.)[J].Jiangsu Agricultural Sciences,2020,48(15):89-94.
点击复制

胡萝卜1个新4CL基因的克隆、进化及表达分析(PDF)
分享到：

《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第48卷
期数:
2020年第15期
页码:
89-94
栏目:
生物技术
出版日期:
2020-08-05

文章信息/Info

Title:
Cloning，evolution and expression of a new 4-coumarate：CoA ligase gene in carrot (Daucus carota L.)
作者:
罗庆; 钟秀来; 卢松; 王天文; 孟平红; 谭国飞
贵州省农业科学院园艺研究所，贵州贵阳 550006
Author(s):
Luo Qing; et al
关键词:
胡萝卜; 4CL基因; 克隆; 进化; 表达
Keywords:
-
分类号:
S631.201
DOI:
-
文献标志码:
A
摘要:
胡萝卜(Daucus carota L.)在NCBI(美国国家生物信息中心)上总共公布了13个4-香豆酰-CoA连接酶(4CL)基因，而在芹菜转录数据中发现1个4CL基因，利用该基因序列设计1对克隆引物，成功得到1个新胡萝卜4CL基因，命名为Dc4CL-1。Dc4CL-1基因长1 635 bp，编码544个氨基酸，位于胡萝卜基因组第2染色体上。进化树分析显示，Dc4CL-1基因聚集在与木质素合成有关的4CL Ⅰ亚族。实时荧光定量PCR转录表达发现，在胡萝卜根生长发育期间，Dc4CL-1基因在野生胡萝卜中高表达，而在栽培胡萝卜中低表达。推测Dc4CL-1基因与胡萝卜木质素合成有关。
Abstract:
-

参考文献/References:

［1］Tan G F，Wang F，Zhang X Y，et al. Different lengths，copies and expression levels of the mitochondrial atp6 gene in male sterile andfertile lines of carrot(Daucus carota L.)［J］. Mitochondrial DNA，2018，29(3)：446-454.
［2］Bergh B O. Genetic improvement of vegetable crops［M］. Amsterdam：Pergamon Press，1993：479-484.
［3］Wang G L，Huang Y，Zhang X Y，et al. Transcriptome-based identification of genes revealed differential expression profiles and ligninaccumulation during root development in cultivated and wild carrots［J］. Plant Cell Reports，2016，35(8)：1743-1755.
［4］Ehlting J，Buttner D，Wang Q，et al. Three 4-coumarate：coenzyme A ligases in Arabidopsis thaliana represent two evolutionarily divergent classes in angiosperms［J］. Plant Journal，1999，19(1)：9-20.
［5］Cukovic D，Ehlting J，Vanziffle J A，et al. Structure and evolution of 4-coumarate：coenzyme A ligase (4CL)gene families［J］. Biological Chemistry，2001，82(4)：645-654.
［6］Hisano H，Nandakumar R，Wang Z Y. Genetic modification of lignin biosynthesis for improved biofuel production［J］. In Vitro Cellular & Developmental Biology，2009，l45(3)：306-313.
［7］Hu Y，Gai Y，Yin L，et al. Crystal structures of a Populus tomentosa 4-coumarate：CoA ligase shed light on its enzymatic mechanisms［J］. Plant Cell，2010，22(9)：3093-3104.
［8］Fraser C M，Chapple C. The phenylpropanoid pathway in Arabidopsis［J］. The Arabidopsis Book，2011，9：e0152.
［9］Iorizzo M，Ellison S，Senalik D，et al. A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution［J］. Nature Genetics，2016，48(6)：657-666.
［10］Jia X L，Wang G L，Xiong F，et al. De novo assembly，transcriptome characterization，lignin accumulation，and anatomic characteristics：novel insights into lignin biosynthesis during celery leaf development［J］. Scientific Reports，2015，5：8259.
［11］Kumar S，Nei M，Dudley J，et al. MEGA：a biologist-centric software for evolutionary analysis of DNA and protein sequences［J］. Briefings in Bioinformatics，2008，9(4)：299-306.
［12］Xu Z S，Huang Y，Wang F，et al. Transcript profiling of structural genes involved in cyanidin-based anthocyanin biosynthesis between purple and non-purple carrot (Daucus carota L.) cultivars reveals distinct patterns［J］. BMC Plant Biology，2014，14(1)：262.
［13］Tian C，Jiang Q，Wang F，et al. Selection of suitable reference genes for qPCR normalization under abiotic stresses and hormone stimuli in carrot leaves［J］. PLoS One，2015，10(2)：e0117569.
［14］Xu Z S，Tan H W，Wang F，et al. CarrotDB：a genomic and transcriptomic database for carrot［J］. Database，2014，2014：bau096.
［15］Souza C A，Barbazuk B，Ralph S G，et al. Genome-wide analysis of a land plant-specific acyl：coenzyme A synthetase(ACS)gene family in Arabidopsis，poplar，rice and Physcomitrella［J］. New Phytologist，2008，179(4)：987-1003.
［16］Tan G F，Wang F，Ma J，et al. Identification and analysis of the characteristics of a wild petaloid male-sterile carrot Wuye-BY［J］. Plant Genet Resour，2017，18(6)：1216-1220.
［17］Wang G L，Que F，Xu Z S，et al. Exogenous gibberellin enhances secondary xylem development and lignification in carrot taproot［J］. Protoplasma，2017，254(2)：839-848.
［18］Dixon R A，Chen F，Guo D，et al. The biosynthesis of monolignols：a“metabolic grid”，or independent pathways to guaiacyl and syringyl units?［J］. Phytochemistry，2001，57(7)：1069-1084.
［19］Koskinen O. Catalytic hydrogenation and hydrogenolysis of lignin model compounds in ionic liquid environment［D］. Helsinki：University of Helsinki，2017.
［20］陈磊，陈汉平，陆强，等. 木质素结构及热解特性［J］. 化工学报，2014，65(9)：3626-3633.
［21］Schafer J，Trierweiler B，Bunzel M. Maturation-related changes of carrot lignins［J］. Journal of the Science of Food and Agriculture，2018，98(3)：1016-1023.
［22］Howard L R，Griffin L E. Lignin formation and surface discoloration of minimally processed carrot sticks［J］. Journal of Food Science，1993，58(5)：1065-1067.
［23］舒海燕，孙威，魏卿，等. 香蕉4-CL基因Mu4CL15的功能鉴定［J］. 基因组学与应用生物学，2017，36(8)：3025-3033.
［24］王广龙. 胡萝卜肉质根发育过程中激素和品质的变化规律研究［D］. 南京：南京农业大学，2016.
［25］Hu W J，Kawaoka A，Tsai C J，et al. Compartmentalized expression of two structurally and functionally distinct 4-coumarate：CoA ligase genes in aspen (Populus tremuloides)［J］. Proceedings of the National Academy of ences，1998，95(9)：5407-5412.
［26］夏栋楠，朱家红，李辉亮，等. 海南龙血树4-香豆酸辅酶A连接酶基因的克隆与表达分析［J］. 分子植物育种，2018，16(23)：7656-7662.
［27］Gui J S，Shen J H，Li L G. Functional characterization of evolutionarily divergent 4-coumarate：coenzyme a ligases in rice［J］. Plant Physiology，2011，157(2)：574-586.

相似文献/References:

[1]朱代根,陈君若,李明.胡萝卜热风干燥过程热质耦合传递分析[J].江苏农业科学,2014,42(02):201.
　Zhu Daigen,et al.Analysis of coupled heat and mass transfer during hot air drying of carrot[J].Jiangsu Agricultural Sciences,2014,42(15):201.
[2]丁青芝,白薇,范婷婷,等.草莓-胡萝卜复合果蔬汁的加工工艺及悬浮稳定性[J].江苏农业科学,2014,42(06):242.
　Ding Qingzhi,et al.Processing technology and suspension stability of strawberry-carrot compound juice[J].Jiangsu Agricultural Sciences,2014,42(15):242.
[3]徐亚元,郎旭敏,肖亚冬,等.超声渗透辅助组合干燥对胡萝卜干燥均匀性及品质的影响[J].江苏农业科学,2021,49(9):148.
　Xu Yayuan,et al.Effects of ultrasonic infiltration combined with drying on uniformity and quality of carrot chips[J].Jiangsu Agricultural Sciences,2021,49(15):148.
[4]闫学敏,吴英华,史艳,等.胡萝卜YUCCA基因家族鉴定及生物信息学分析[J].江苏农业科学,2021,49(23):52.
　Yan Xuemin,et al.Identification and bioinformatics analysis of YUCCA gene family in carrots (Daucus carota var. sativa DC.)[J].Jiangsu Agricultural Sciences,2021,49(15):52.
[5]赵宇璇,刘真真,常双锋,等.胡萝卜叶色突变体叶色参数和色素含量及其相关性分析[J].江苏农业科学,2022,50(4):100.
　Zhao Yuxuan,et al.Analysis of correlation between leaf color parameters and pigment contents of carrot leaf color mutants[J].Jiangsu Agricultural Sciences,2022,50(15):100.
[6]黎叶,金奇年,张建民,等.基于年龄-阶段两性生命表的不同寄主对草地贪夜蛾生长发育和繁殖力的影响[J].江苏农业科学,2024,52(6):136.
　Li Ye,et al.Impacts of different hosts on development and fecundity of Spodoptera frugiperda based on age-stage two-sex life table[J].Jiangsu Agricultural Sciences,2024,52(15):136.

备注/Memo

备注/Memo:
收稿日期：2020-04-30
基金项目：贵州省科技支撑计划［编号：黔科合支撑(2019)2257号］；贵州省科技计划［编号：黔科合平台人才(2018)5768-03］；贵州省科技计划［编号：黔科合基础(2019)1307号］；贵州省科技支撑计划［编号：黔科合支撑（2016）2599号］；贵州省科研机构服务企业行动计划［编号：黔科合平台人才（2017）5715-08］；贵州省农业科学院蔬菜项目［编号：黔农科院种质资源（2020）10号］；贵州省高层次创新型人才培养“百”层次人才项目［编号：黔科合人才（2015）4024号］。
作者简介：罗庆(1987—)，女，四川资阳人，硕士，研究实习员，主要从事分子生物学研究。E-mail：lqing985@foxmail.com。
通信作者：谭国飞，博士，副研究员，主要从事伞形科蔬菜遗传育种与分子遗传学研究。E-mail：tgfei@foxmail.com。
更新日期/Last Update: 2020-08-05