|本期目录/Table of Contents|

[1]李星坤,潘慧,李攀,等.基于CRISPR/Cas9系统的拟南芥ugt84a1/ugt84a2双突变体制作及突变位点分析[J].江苏农业科学,2020,48(20):49-55.
　Li Xingkun,et al.Construction of Arabidopsis ugt84a1/ugt84a2 double mutant and analysis of mutation site based on CRISPR/Cas9 system[J].Jiangsu Agricultural Sciences,2020,48(20):49-55.
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基于CRISPR/Cas9系统的拟南芥ugt84a1/ugt84a2双突变体制作及突变位点分析(PDF)
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第48卷
期数:
2020年第20期
页码:
49-55
栏目:
生物技术
出版日期:
2020-10-20

文章信息/Info

Title:
Construction of Arabidopsis ugt84a1/ugt84a2 double mutant and analysis of mutation site based on CRISPR/Cas9 system
作者:
李星坤1; 潘慧1; 李攀2; 王振1; 刘林1; 张桂芝1
1.临沂大学药学院，山东临沂 276000； 2.聊城大学药学院，山东聊城 252000
Author(s):
Li Xingkun; et al
关键词:
拟南芥; 双突变体; CRISPR/Cas9; 基因敲除; 位点分析
Keywords:
-
分类号:
S184
DOI:
-
文献标志码:
A
摘要:
CRISPR/Cas9基因编辑技术相对于锌指核酸酶(ZFNs)和转录激活因子效应物核酸酶(TALENs)而言，具有操作简便、突变效率高等优点，已经被广泛应用于医学、动物科学、植物科学等领域。拟南芥糖基转移酶UGT84A1、UGT84A2参与植物次生代谢及外源毒物反应，并且为同工酶。本研究以拟南芥糖基转移酶同工酶基因UGT84A1和UGT84A2为靶向基因，构建CRISPR/Cas9双突变体表达载体，并转化到农杆菌浸染拟南芥，从而同时定向敲除靶向基因。根据拟南芥转基因后代的测序结果，对获得的42株阳性转化植株进行突变位点分析，结果表明，有2株阳性植株发生双突变，由此成功构建了ugt84a1/ugt84a2双突变体。试验结果可为加快ugt84a1/ugt84a2功能基因资源的开发利用提供有力的理论与方法支持。
Abstract:
-

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备注/Memo

备注/Memo:
收稿日期：2020-06-18
基金项目：国家自然科学基金(编号：31700238)；山东省自然科学基金(编号：ZR2017PC007)。
作者简介：李星坤(1996—)，男，山东潍坊人，主要研究方向为植物小分子糖基化修饰。E-mail：sdwflxk111@163.com。
通信作者：张桂芝，博士，教授，主要从事植物分子遗传学研究。E-mail：guizhizhang11@163.com。
更新日期/Last Update: 2020-11-09