|本期目录/Table of Contents|

[1]施天元,程玉祥.杨树PtBBE9a基因异源过表达功能分析[J].江苏农业科学,2020,48(23):66-70.
　Shi Tianyuan,et al.Functional analysis of overexpression of populus PtBBE9a in Arabidopsis[J].Jiangsu Agricultural Sciences,2020,48(23):66-70.
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杨树PtBBE9a基因异源过表达功能分析(PDF)
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第48卷
期数:
2020年第23期
页码:
66-70
栏目:
生物技术
出版日期:
2020-12-05

文章信息/Info

Title:
Functional analysis of overexpression of populus PtBBE9a in Arabidopsis
作者:
施天元; 程玉祥
东北林业大学林木遗传育种国家重点实验室，黑龙江哈尔滨 150040
Author(s):
Shi Tianyuan; et al
关键词:
杨树; PtBBE9a基因; 异源过表达; 启动子; 转基因拟南芥; BBE
Keywords:
-
分类号:
S718.43
DOI:
-
文献标志码:
A
摘要:
小檗碱桥酶(berberine bridge enzyme，简称BBE)是一类典型的黄素双共价氧化酶家族，参与植物生长发育的多个生物过程。PtBBE9a是笔者所在课题组前期从杨树发育木质部分离、鉴定的糖蛋白。PtBBE9a基因表达在杨树木质部呈现高丰度水平，并且随着茎木质化程度的增强而升高。组织β-葡萄糖苷酸酶(GUS)活性染色结果显示，PtBBE9a启动子活性集中在ProPtBBE9a：：GUS转基因拟南芥幼苗的顶端分生组织、成熟果荚皮及根组织。通过构建PtBBE9a-GFP过表达载体发现，PtBBE9a过表达拟南芥比野生型拟南芥早开花。进一步研究发现，转基因拟南芥的叶片数少于野生型拟南芥，而其茎木质素含量高于野生型拟南芥。推测PtBBE9a可能通过调控拟南芥生长发育周期而产生早花表型。
Abstract:
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参考文献/References:

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备注/Memo

备注/Memo:
收稿日期：2020-04-20
基金项目：国家自然科学基金面上项目(编号：31770637)。
作者简介：施天元(1989—)，女，黑龙江哈尔滨人，硕士，主要从事林木遗传与育种研究。E-mail：shitianyuan@nefu.edu.cn。
通信作者：程玉祥，博士，教授，主要从事林木遗传与育种研究。E-mail：chengyuxiang@nefu.edu.cn。
更新日期/Last Update: 2020-12-05