|本期目录/Table of Contents|

[1]田娟,张 曼,孙墨可,等.不同基因型及培养基在燕麦花药组织培养中的应用[J].江苏农业科学,2021,49(9):49-53.
 Tian Juan,et al.Application of different genotypes and media in anther tissue culture of oat[J].Jiangsu Agricultural Sciences,2021,49(9):49-53.
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不同基因型及培养基在燕麦花药组织培养中的应用(PDF)
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第49卷
期数:
2021年第9期
页码:
49-53
栏目:
生物技术
出版日期:
2021-05-05

文章信息/Info

Title:
Application of different genotypes and media in anther tissue culture of oat
作者:
田娟 张 曼 孙墨可 董玉迪 王春龙 郭来春 魏黎明 任长忠
吉林省白城市农业科学院,吉林白城 137000
Author(s):
Tian Juanet al
关键词:
燕麦基因型培养基花药组织培养愈伤组织
Keywords:
-
分类号:
S339.4+1;S512.604.3
DOI:
-
文献标志码:
A
摘要:
以JA3、M44、19A647燕麦资源为试验材料,采用花药离体培养方法,将3种材料的花药接种于C17、W14培养基上,研究3种基因型在2种培养基上反应率、出愈率、转移率和再生率的差异,以期筛选出适合燕麦花药培养的基因型和诱导培养基。结果表明,在3个基因型中,JA3的反应率、出愈率、转移率都是最高的,分别达到36.29%、2695%、86.93%,只有JA3获得了再生植株,其绿苗率为0.10%,可见JA3适合用于花药组织培养,可以作为燕麦花药培养的桥梁材料;C17培养基中的愈伤组织比较紧凑,质量较好,W14培养基中的愈伤组织不易聚集长大,质量较差,从W14培养基中共获得6株白化苗,从C17培养基中获得2株白化苗、2株绿苗。由研究结果可以看出,燕麦花药愈伤组织诱导和再分化在基因型之间的差异显著,JA3适合作为燕麦花药培养的桥梁品种,相较于W14培养基,C17为燕麦花药培养的最佳培养基。
Abstract:
-

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备注/Memo

备注/Memo:
收稿日期:2020-08-11
基金项目:国家燕麦荞麦产业技术体系建设专项(编号:CARS-08)。
作者简介:田娟(1985—),女,吉林松原人,硕士,助理研究员,主要从事生物技术育种研究。E-mail:tianjuanyes@126.com。
通信作者:任长忠,博士,研究员,从事燕麦荞麦育种研究。E-mail:13500830080@163.com。
更新日期/Last Update: 2021-05-05