|本期目录/Table of Contents|

[1]石桃雄,汪燕,梁成刚,等.苦荞二磷酸尿核苷-半乳糖转运载体FtUTR的鉴定与分析[J].江苏农业科学,2021,49(15):53-57.
 Shi Taoxiong,et al.Identification and analysis of UDP-galactose transporter FtUTR in tartary buckwheat[J].Jiangsu Agricultural Sciences,2021,49(15):53-57.
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苦荞二磷酸尿核苷-半乳糖转运载体FtUTR的鉴定与分析(PDF)
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第49卷
期数:
2021年第15期
页码:
53-57
栏目:
生物技术
出版日期:
2021-08-05

文章信息/Info

Title:
Identification and analysis of UDP-galactose transporter FtUTR in tartary buckwheat
作者:
石桃雄汪燕梁成刚韦春玉关志秀黄娟朱丽伟
贵州师范大学生命科学学院/荞麦产业技术研究中心,贵州贵阳 550001
Author(s):
Shi Taoxionget al
关键词:
苦荞二磷酸尿核苷-半乳糖转运体蛋白序列分析基因表达
Keywords:
-
分类号:
S517.01
DOI:
-
文献标志码:
A
摘要:
植物二磷酸尿核苷-半乳糖转运蛋白(UDP-galactose transporter,UTR)参与介导UDP-半乳糖的跨膜运输,对于高尔基体内非纤维素多糖和糖蛋白合成至关重要。为了给苦荞糖运输及调控机制研究提供科学参考,本研究基于基因功能注释与同源基因比对,鉴定到9个苦荞FtUTR基因,其序列长度在1 920~5 975 bp间,氨基酸残基在 81~352个间。FtUTR氨基酸序列的一致性为19.14%,说明苦荞不同FtUTR蛋白间序列保守性较低。但多个苦荞FtUTR可分别与同源的拟南芥AtUTR聚在一起,说明不同物种间UTR蛋白的进化关系较为紧密。基于茎的转录组测序鉴定到5个差异表达基因(DEGs),其中FtPinG[STBX]0001931200.01[STBZ]在出苗后5 d高表达;FtPinG000[STBX]1901300.01和FtPinG0002689200.01[STBZ]在出苗后10 d高表达;FtPinG[STBX]0001931400.01和FtPinG0001052700.01[STBZ]在出苗后15 d高表达,推测它们可能在幼苗的特定发育阶段发挥作用。另外,幼苗发育过程中苦荞DEGs的表达量存在多个显著正相关或负相关关系。本研究结果可为下一步深入探索苦荞FtUTR调控UDP-半乳糖运输以及生长发育提供基础。
Abstract:
-

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:20201-04-14
基金项目:国家自然科学基金 (编号:32060511);贵州省科技支撑计划 (编号:黔科合ZC[2019]2298);贵州省高层次创新型人才“千层次人才”项目;贵州省荞麦种质资源保育及创新重点实验室建设基金(编号:黔教合KY[2017]002);贵州省研究生教育创新计划 (编号:黔教合YJSCXJH[2020]100)。
作者简介:石桃雄(1980—),女,宁夏石嘴山人,博士,副教授,研究方向为荞麦数量遗传学,E-mail:shitaoxiong@126.com;共同第一作者:汪燕(1985—),女,重庆人,博士,副教授,研究方向为荞麦种质资源创新与遗传育种,E-mail:yanwanguf@163.com。
通信作者:梁成刚,博士,副教授,研究方向为荞麦遗传育种。E-mail:jesselcg@163.com。
更新日期/Last Update: 2021-08-05