|本期目录/Table of Contents|

[1]高帆,宋韡.基于AFLP标记的苦荞种质资源遗传多样性研究[J].江苏农业科学,2016,44(05):122-126.
 Gao Fan,et al.Study on genetic diversity of tartary buckwheat germplasm resources based on AFLP markers[J].Jiangsu Agricultural Sciences,2016,44(05):122-126.
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第44卷
期数:
2016年05期
页码:
122-126
栏目:
遗传育种与耕作栽培
出版日期:
2016-05-25

文章信息/Info

Title:
Study on genetic diversity of tartary buckwheat germplasm resources based on AFLP markers
作者:
高帆1 宋韡2
1.山西大学生命科学学院,山西太原 030006; 2.山西体育职业学院,山西太原 030006
Author(s):
Gao Fanet al
关键词:
苦荞AFLP遗传多样性
Keywords:
-
分类号:
S517.024
DOI:
-
文献标志码:
A
摘要:
用AFLP分子标记手段对我国苦荞种质资源遗传多样性进行研究,为综合评价我国苦荞种质资源提供依据。以50份苦荞核心种质为试验材料,优化筛选出19对AFLP标记分析引物,经AFLP-PCR扩增、PAGE检测,统计分析AFLP图谱,运行Popgene Ver.1.31和NTSYSpc Ver.2.2软件对苦荞种质资源遗传多样性和遗传关系进行分析,共检测到211个AFLP特异性标记,平均每对引物组合产生11.11个多态性位点(PIC),遗传相似系数(GS)分布区间均较大,变幅为0.515~0.954,辛普森指数和香农指数计算结果表明来自四川的苦荞种质资源多样性最为丰富;Popgene Ver.1.31运行结果表明,当GS为0.790时,50份苦荞材料被分为5个组群,聚类结果与苦荞种质的地理分布有一定的相关性。说明AFLP是一种有效的分子标记方式,适合于苦荞种质遗传多样性分析;我国苦荞种质资源多样性丰富,须要在保护、利用现有种质资源的基础上继续加强区域间引种、育种,为我国荞麦产业的发展提供帮助。
Abstract:
-

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备注/Memo

备注/Memo:
收稿日期:2015-04-21
基金项目:国家科技支撑计划(编号:2011BAD09B01)。
作者简介:高帆(1985—),男,山西长治人,博士,实验师,主要从事荞麦种质资源遗传多样性研究。E-mail:gaofan@sxu.edu.cn。
更新日期/Last Update: 2016-05-25