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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:

第50卷

期数:

2022年第3期

页码:

1-8

栏目:

“转录组”专题

出版日期:

2022-02-05

文章信息/Info

Title:

Comparative transcriptome analysis of different resistant lotus (Nelumbo nucifera) cultivars under mixed saline-alkali stress

作者:

刘艺平¹; 2;  张一琪¹; 苏 少文¹;  刘红利¹; 2;  贺丹¹; 2;  孔德政¹; 2

1.河南农业大学风景园林与艺术学院，河南郑州 450000； 2.河南省优质花卉蔬菜种苗工程研究中心，河南郑州 450000

Author(s):

Liu Yiping; et al

关键词:

荷花; 盐碱胁迫; 转录组; 差异基因; 分子机制

Keywords:

-

分类号:

S682.320.1

DOI:

-

文献标志码:

A

摘要:

为研究荷花的耐盐碱机制，以混合盐碱胁迫处理前后的抗性荷花品种黄帅TO及敏感品种台湾磨盘莲SE为试验材料，进行转录组测序，并运用qRT-PCR 法验证测序结果的准确性。2个品种在混合盐碱胁迫下共检测到 4 916 个差异表达基因，其中，上调表达基因3 101个，下调表达基因1 815个，对抗性品种的49个特有差异基因进行分析发现，与细胞壁合成有关基因在耐盐碱胁迫中起着重要的作用；GO富集分析发现，敏感品种与抗性品种差异基因富集最多的条目相同，但敏感品种富集的差异基因数目更多，推测不同材料间基因功能的差异在决定材料特性时发挥着重要作用；KEGG通路分析表明，苯丙烷生物合成通路在抗性品种对盐碱胁迫的响应方面发挥了重要作用，而敏感品种抗性弱主要是由于其碳代谢系统紊乱。qRT-PCR结果表明，本次转录组测序结果可靠。荷花耐盐碱胁迫过程是一个复杂的生物学过程，包括生物过程、细胞组分和分子功能多个方面的基因参与，细胞壁合成有关基因是提高荷花耐盐碱能力的关键基因，苯丙烷生物合成、碳代谢很可能是造成2个荷花品种耐盐碱能力差异大小的重要原因。

Abstract:

-

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备注/Memo

备注/Memo:

收稿日期：2021-09-12
基金项目：国家自然科学基金(编号：31600568)；河南省高等学校重点研究项目(编号：21A220003)；河南农业大学科技创新基金(编号： KJCX2017C01)。
作者简介：刘艺平(1977—)，女，河南温县人，博士，副教授，主要从事荷花耐盐碱胁迫方面的研究。E-mail：lyp_163@163.com。
通信作者：孔德政，博士，教授，主要从事荷花耐盐碱胁迫方面的研究。 E-mail：kdz217@163.com。

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更新日期/Last Update: 2022-02-05