|本期目录/Table of Contents|

[1]顾鹏鹏,马鑫磊,姚锐,等.谷子HSP90基因家族鉴定及干旱胁迫下表达分析[J].江苏农业科学,2022,50(6):45-52.
　Gu Pengpeng,et al.Identification and expression analysis of HSP90 genes family in foxtail millet (Setaria italica L.) under drought stress[J].Jiangsu Agricultural Sciences,2022,50(6):45-52.
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谷子HSP90基因家族鉴定及干旱胁迫下表达分析(PDF)
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第50卷
期数:
2022年第6期
页码:
45-52
栏目:
生物技术
出版日期:
2022-03-20

文章信息/Info

Title:
Identification and expression analysis of HSP90 genes family in foxtail millet (Setaria italica L.) under drought stress
作者:
顾鹏鹏;  马鑫磊;  姚锐;  高慧;  林小虎
河北省作物逆境生物学重点实验室/河北科技师范学院农学与生物科技学院，河北秦皇岛 066004
Author(s):
Gu Pengpeng; et al
关键词:
热激蛋白90; 基因家族; 干旱胁迫; 表达分析; 谷子
Keywords:
-
分类号:
S515.01
DOI:
-
文献标志码:
A
摘要:
热激蛋白90 (heat shock proteins 90，HSP90) 是广泛存在于植物中的一种高度保守的细胞伴侣蛋白家族，参与调节和维持各种蛋白质的构象，在植物的生物和非生物胁迫应激反应中起着重要作用。为探讨谷子HSP90基因家族(SiHSP90s)在干旱胁迫下的潜在抗旱功能，对SiHSP90s进行鉴定，并分析其在干旱胁迫下的表达情况。共鉴定出9个SiHSP90基因分别位于谷子5条染色体上。理化性质分析显示SiHSP90s蛋白长度为403～817个氨基酸，分子量为46.1～92.7 ku。系统进化分析显示SiHSP90s分为Ⅰ型和Ⅱ型2个亚家族。基因结构与蛋白基序分析显示，Ⅰ型和Ⅱ型亚族成员的外显子数量分别在2～3个和12～20个之间，且均含有保守的HATPase_c(PF02518)和HSP90(PF00183)结构域。顺式元件分析表明，SiHSP90s基因可以应对不同的非生物胁迫。qRT-PCR结果表明，干旱胁迫下SiHSP90.2和SiHSP90.3转录水平极显著上调。这些结果为阐明SiHSP90家族的进化关系以及进一步研究SiHSP90基因的功能特性提供了有价值的信息。
Abstract:
-

参考文献/References:

［1］Chaves M M，Maroco J P，Pereira J S. Understanding plant responses to drought-from genes to the whole plant［J］. Functional Plant Biology，2003，30(3)：239-264.
［2］Fatima A，Hussain S，Hussain S，et al. Differential morphophysiological，biochemical，and molecular responses of maize hybrids to salinity and alkalinity stresses［J］. Agronomy，2021，11(6)：1150.
［3］Nakashima K，Yamaguchi-Shinozaki K.Regulons involved in osmotic stress-responsive and cold stress-responsive gene expression in plants［J］. Physiologia Plantarum，2006，126(1)：62-71.
［4］Gao G Z，Hu J H，Zhang X J，et al. Transcriptome analysis reveals genes expression pattern of seed response to heat stress in Brassica napus L.［J］. Oil Crop Science，2021，6(2)：87-96.
［5］Sangster T A，Queitsch C.The HSP90 chaperone complex，an emerging force in plant development and phenotypic plasticity［J］. Current Opinion in Plant Biology，2005，8(1)：86-92.
［6］Al-Whaibi M H.Plant heat-shock proteins：a mini review［J］. Journal of King Saud University-Science，2011，23(2)：139-150.
［7］Pearl L H，Prodromou C.Structure and in vivo function of Hsp90［J］. Current Opinion in Structural Biology，2000，10(1)：46-51.
［8］Terasawa K，Minami M，Minami Y.Constantly updated knowledge of Hsp90［J］. Journal of Biochemistry，2005，137(4)：443-447.
［9］Pearl L H，Prodromou C.Structure and mechanism of the Hsp90 molecular chaperone machinery［J］. Annual Review of Biochemistry，2006，75：271-294.
［10］Hainzl O，Lapina M C，Buchner J，et al. The charged linker region is an important regulator of Hsp90 function［J］. Journal of Biological Chemistry，2009，284(34)：22559-22567.
［11］Sung N，Lee J，Kim J H，et al. Mitochondrial Hsp90 is a ligand-activated molecular chaperone coupling ATP binding to dimer closure through a coiled-coil intermediate［J］. PNAS，2016，113(11)：2952-2957.
［12］Raman S，Suguna K.Functional characterization of heat-shock protein 90 from Oryza sativa and crystal structure of its N-terminal domain［J］. Acta Crystallographica.Section F，Structural Biology Communications，2015，71(Pt 6)：688-696.
［13］Morán Luengo T，Mayer M P，Rüdiger S G D.The Hsp70-Hsp90 chaperone cascade in protein folding［J］. Trends in Cell Biology，2019，29(2)：164-177.
［14］Shinozaki F，Minami M，Chiba T，et al. Depletion of Hsp90 β induces multiple defects in B cell receptor signaling［J］. The Journal of Biological Chemistry，2006，281(24)：16361-16369.
［15］Zuehlke A，Johnson J L.Hsp90 and co-chaperones twist the functions of diverse client proteins［J］. Biopolymers，2010，93(3)：211-217.
［16］Agarwal G，Garg V，Kudapa H，et al. Genome-wide dissection of AP2/ERF and HSP90 gene families in five legumes and expression profiles in chickpea and pigeonpea［J］. Plant Biotechnology Journal，2016，14(7)：1563-1577.
［17］Grigorova B，Vaseva I，Demirevska K，et al. Combined drought and heat stress in wheat：changes in some heat shock proteins［J］. Biologia Plantarum，2011，55(1)：105-111.
［18］di Donato M，Geisler M.HSP90 and co-chaperones：a multitaskers view on plant hormone biology［J］. FEBS Letters，2019，593(13)：1415-1430.
［19］Song H M，Zhao R M，Fan P X，et al. Overexpression of AtHsp90.2，AtHsp90.5 and AtHsp90.7 in Arabidopsis thaliana enhances plant sensitivity to salt and drought stresses［J］. Planta，2009，229(4)：955-964.
［20］Song Z P，Pan F L，Yang C，et al. Genome-wide identification and expression analysis of HSP90 gene family in Nicotiana tabacum［J］. BMC Genetics，2019，20(1)：35.
［21］Lv D W，Subburaj S，Cao M，et al. Proteome and phosphoproteome characterization reveals new response and defense mechanisms of Brachypodium distachyon leaves under salt stress［J］. Molecular & Cellular Proteomics，2014，13(2)：632-652.
［22］Hao P C，Zhu J T，Gu A Q，et al. An integrative proteome analysis of different seedling organs in tolerant and sensitive wheat cultivars under drought stress and recovery［J］. Proteomics，2015，15(9)：1544-1563.
［23］Nadeem F，Ahmad Z，Ul Hassan M，et al. Adaptation of foxtail millet (Setaria italica L.) to abiotic stresses：a special perspective of responses to nitrogen and phosphate limitations［J］. Frontiers in Plant Science，2020，11：187.
［24］Lata C R，Gupta S，Prasad M.Foxtail millet：a model crop for genetic and genomic studies in bioenergy grasses［J］. Critical Reviews in Biotechnology，2013，33(3)：328-343.
［25］Muthamilarasan M，Prasad M.Advances in Setaria genomics for genetic improvement of cereals and bioenergy grasses［J］. Theoretical and Applied Genetics，2015，128(1)：1-14.
［26］Bennetzen J L，Schmutz J，Wang H，et al. Reference genome sequence of the model plant Setaria［J］. Nature Biotechnology，2012，30(6)：555-561.
［27］Zhang M，Shen Z W，Meng G Q，et al. Genome-wide analysis of the Brachypodium distachyon (L.) P. Beauv. Hsp90 gene family reveals molecular evolution and expression profiling under drought and salt stresses［J］. PLoS One，2017，12(12)：e0189187.
［28］Xu J Y，Xue C C，Xue D，et al. Overexpression of GmHsp90s，a heat shock protein 90 (Hsp90) gene family cloning from soybean，decrease damage of abiotic stresses in Arabidopsis thaliana［J］. PLoS One，2013，8(7)：e69810.
［29］Zai W S，Miao L X，Xiong Z L，et al. Comprehensive identification and expression analysis of Hsp90s gene family in Solanum lycopersicum［J］. Genetics and Molecular Research，2015，14(3)：7811-7820.
［30］Zhang K J，He S S，Sui Y H，et al. Genome-wide characterization of HSP90 gene family in cucumber and their potential roles in response to abiotic and biotic stresses［J］. Frontiers in Genetics，2021，12：584886.
［31］刘云飞，万红建，杨悦俭，等. 番茄热激蛋白90的全基因组鉴定及分析［J］. 遗传，2014，36(10)：1043-1052.
［32］Yamada K，Fukao Y，Hayashi M，et al. Cytosolic HSP90 regulates the heat shock response that is responsible for heat acclimation in Arabidopsis thaliana［J］. The Journal of Biological Chemistry，2007，282(52)：37794-37804.
［33］Liu H，Lyu H M，Zhu K K，et al. The emergence and evolution of intron-poor and intronless genes in intron-rich plant gene families［J］. The Plant Journal，2021，105(4)：1072-1082.
［34］Jin Z W，Chandrasekaran U，Liu A Z.Genome-wide analysis of the Dof transcription factors in Castor bean (Ricinus communis L.)［J］. Genes & Genomics，2014，36(4)：527-537.
［35］LaPointe P，Mercier R，Wolmarans A.Aha-type co-chaperones：the alpha or the Omega of the Hsp90 ATPase cycle？［J］. Biological Chemistry，2020，401(4)：423-434.
［36］Meyer P，Prodromou C，Hu B，et al. Structural and functional analysis of the middle segment of Hsp90：implications for ATP hydrolysis and client protein and cochaperone interactions［J］. Molecular Cell，2003，11(3)：647-658.
［37］Yang Z F，Zhou Y，Wang X F，et al. Genomewide comparative phylogenetic and molecular evolutionary analysis of tubby-like protein family in Arabidopsis，rice，and poplar［J］. Genomics，2008，92(4)：246-253.
［38］Muppala S，Gudlavalleti P K，Malireddy K R，et al. Development of stable transgenic maize plants tolerant for drought by manipulating ABA signaling through Agrobacterium-mediated transformation［J］. Journal，Genetic Engineering & Biotechnology，2021，19(1)：96.

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备注/Memo

备注/Memo:
收稿日期：2021-07-06
基金项目：国家“十三五”重点研发计划(编号：2019YFD1001701-2)；河北省现代农业产业技术体系创新团队(杂粮杂豆)项目(编号：HBCT2018070404)；河北省自然科学基金(编号：C2019407001)；河北省高等学校科学技术研究项目(编号：QN2020154)；河北省在读研究生创新能力培养资助项目(编号：CXZZSS2021152)。
作者简介：顾鹏鹏(1994—)，男，山东滨州人，硕士研究生，主要从事植物分子遗传与基因工程研究。E-mail：1006555310@qq.com。
通信作者：林小虎，博士，教授，主要从事植物遗传学研究。E-mail：xiaohulin2008@163.com。
更新日期/Last Update: 2022-03-20