|本期目录/Table of Contents|

[1]杜军华,姜东伯,张津京,等.青藏高原东部22株栽培与野生羊肚菌的分子进化分析[J].江苏农业科学,2022,50(7):28-34.
 Du Junhua,et al.Molecular evolution analysis of 22 cultivated and wild Morchella strains in eastern Qinghai—Tibet Plateau[J].Jiangsu Agricultural Sciences,2022,50(7):28-34.
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青藏高原东部22株栽培与野生羊肚菌的分子进化分析(PDF)
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第50卷
期数:
2022年第7期
页码:
28-34
栏目:
生物技术
出版日期:
2022-04-05

文章信息/Info

Title:
Molecular evolution analysis of 22 cultivated and wild Morchella strains in eastern Qinghai—Tibet Plateau
作者:
杜军华1 姜东伯2 张津京3 余建平1 宋长新4 刘甜5 陈辉3 陈群6 吴晓明5
1.青海师范大学生命科学学院,青海西宁 810008; 2.空军军医大学,陕西西安 710032;3.上海农业科学院食用菌研究所,上海 201403; 4.上海城建职业学院计算机系,上海 201415;5.西安交通大学生命科学与技术学院,陕西西安 710049; 6.合肥学院生物食品与环境学院,安徽合肥 230601
Author(s):
Du Junhuaet al
关键词:
子囊菌类羊肚菌分子进化多序列比对青藏高原遗传多样性
Keywords:
-
分类号:
S646.702
DOI:
-
文献标志码:
A
摘要:
为了更好地区分不同羊肚菌菌株,确定其生物多样性,对10个栽培羊肚菌和12个野生羊肚菌进行分析。通过对5个基因区域进行DNA测序,借助数据库检索、多序列比对和分子进化等方法进行分析,发现10种栽培样本属于3种已知物种:六妹羊肚菌(Morchella sextelata)、梯棱羊肚菌(M. importuna)、七妹羊肚菌(M. septimelata)。同GenBank核苷酸序列数据库中已测序样本相比,这些样本都含有一定程度的序列差异,体现在栽培的样本也具有遗传多样性。12个野生型样本同六妹羊肚菌、小海绵羊肚菌(M. spongiola)、Morchella prava物种更接近,并显示出较大的序列差异,其中1株同所有已知羊肚菌品种均有较大的遗传差异。考虑到这些野生羊肚菌的生长地域同其他羊肚菌生长地域的差异,推断由于独特的地理环境,使野生羊肚菌演化出了特有的基因序列。本研究获得了青海一些地区羊肚菌的分类、分子演化和多样性特征,有助于鉴定、选育适合栽培的羊肚菌菌株。
Abstract:
-

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备注/Memo

备注/Memo:
收稿日期:2021-06-15
基金项目:青海省应用基础研究(编号:2018-ZG-790)。
作者简介:杜军华(1965—),男,河北故城人,副教授,主要从事生物技术及应用研究。E-mail:djh319@126.com。
通信作者:杜军华,副教授,主要从事生物技术及应用研究,E-mail:djh319@126.com;吴晓明,博士,副教授,主要从事生物信息研究,E-mail:wxm@mail.xjtu.edu.cn。
更新日期/Last Update: 2022-04-05