|本期目录/Table of Contents|

[1]杨杰,陈蓉,胡文娟,等.甜橙NHX基因家族的鉴定及功能分析[J].江苏农业科学,2022,50(7):35-42.
 Yang Jie,et al.Identification and functional analysis of NHX gene family in Citrus sinensis[J].Jiangsu Agricultural Sciences,2022,50(7):35-42.
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甜橙NHX基因家族的鉴定及功能分析(PDF)
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第50卷
期数:
2022年第7期
页码:
35-42
栏目:
生物技术
出版日期:
2022-04-05

文章信息/Info

Title:
Identification and functional analysis of NHX gene family in Citrus sinensis
作者:
杨杰1 陈蓉1 胡文娟1 吴巧玲1 佟晓楠12 李兴涛12 陈健美12
1.赣南师范大学生命科学学院,江西赣州 341000; 2.国家脐橙工程技术研究中心,江西赣州 341000
Author(s):
Yang Jieet al
关键词:
甜橙NHX基因家族生物信息学基因表达顺式作用元件
Keywords:
-
分类号:
S666.401
DOI:
-
文献标志码:
A
摘要:
Na+/H+逆向转运(Na+/H+exchange or antiporter,NHX)蛋白家族的氨基酸序列具有Na+/H+exchange 蛋白结构域,在植物生长发育和非生物胁迫应答中发挥重要作用。为探究甜橙(Citrus sinensis)NHX基因家族的生物学特性,本研究从甜橙基因组中鉴定出6个NHX基因家族成员,并对其理化性质、基序组成、进化关系、表达谱等进行分析。结果显示,CsNHXs编码蛋白的长度为407~543个氨基酸,分子量为44.71~59.97 ku;所有CsNHXs非均匀地分布在5条染色体上,均具有保守的Na+/H+exchange结构域;蛋白质二级结构分析结果表明,甜橙NHX蛋白主要以 α-螺旋和无规则卷曲为主。与拟南芥(Arabidopsis thaliana)、水稻(Oryza sativa)的NHX蛋白一起构建的系统进化树聚类为3个亚群,甜橙NHX蛋白分布在Endo和Vac亚群中。顺式作用元件预测分析结果表明,CsNHXs启动子区域包含多个植物的生长发育响应元件、激素响应元件和逆境响应元件。转录组数据分析结果表明,CsNHXs在甜橙不同组织中的表达存在差异性,总体上分为特异性表达和普遍性表达。本研究系统分析了甜橙NHX基因家族,为进一步阐明甜橙NHX基因家族的功能提供了理论依据。
Abstract:
-

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:2021-10-17
基金项目:国家自然科学基金(编号:32060667、32160731);江西省教育厅科学技术研究项目(编号:GJJ190758、GJJ180758)。
作者简介:杨杰(1995—),男,山西吕梁人,硕士研究生,主要从事果树逆境生理研究。E-mail:yangjiepersist@qq.com。
通信作者:陈健美,博士,副教授,主要从事柑橘遗传育种研究。E-mail:chen_jm09@hotmail.com。
更新日期/Last Update: 2022-04-05